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Deep sequencing reveals global patterns of mRNA recruitment during translation initiation

Gao, Rong; Yu, Kai; Nie, Jukui; Lian, Tengfei; Jin, Jianshi; Liljas, Anders LU and Su, Xiao Dong LU (2016) In Scientific Reports 6.
Abstract

In this work, we developed a method to systematically study the sequence preference of mRNAs during translation initiation. Traditionally, the dynamic process of translation initiation has been studied at the single molecule level with limited sequencing possibility. Using deep sequencing techniques, we identified the sequence preference at different stages of the initiation complexes. Our results provide a comprehensive and dynamic view of the initiation elements in the translation initiation region (TIR), including the S1 binding sequence, the Shine-Dalgarno (SD)/anti-SD interaction and the second codon, at the equilibrium of different initiation complexes. Moreover, our experiments reveal the conformational changes and regional... (More)

In this work, we developed a method to systematically study the sequence preference of mRNAs during translation initiation. Traditionally, the dynamic process of translation initiation has been studied at the single molecule level with limited sequencing possibility. Using deep sequencing techniques, we identified the sequence preference at different stages of the initiation complexes. Our results provide a comprehensive and dynamic view of the initiation elements in the translation initiation region (TIR), including the S1 binding sequence, the Shine-Dalgarno (SD)/anti-SD interaction and the second codon, at the equilibrium of different initiation complexes. Moreover, our experiments reveal the conformational changes and regional dynamics throughout the dynamic process of mRNA recruitment.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Scientific Reports
volume
6
publisher
Nature Publishing Group
external identifiers
  • scopus:84979730115
  • wos:000380327200001
ISSN
2045-2322
DOI
10.1038/srep30170
language
English
LU publication?
yes
id
36f9d500-7bbc-4627-83bf-a1a5da4fccdd
date added to LUP
2016-12-19 15:07:09
date last changed
2017-09-18 11:32:24
@article{36f9d500-7bbc-4627-83bf-a1a5da4fccdd,
  abstract     = {<p>In this work, we developed a method to systematically study the sequence preference of mRNAs during translation initiation. Traditionally, the dynamic process of translation initiation has been studied at the single molecule level with limited sequencing possibility. Using deep sequencing techniques, we identified the sequence preference at different stages of the initiation complexes. Our results provide a comprehensive and dynamic view of the initiation elements in the translation initiation region (TIR), including the S1 binding sequence, the Shine-Dalgarno (SD)/anti-SD interaction and the second codon, at the equilibrium of different initiation complexes. Moreover, our experiments reveal the conformational changes and regional dynamics throughout the dynamic process of mRNA recruitment.</p>},
  articleno    = {30170},
  author       = {Gao, Rong and Yu, Kai and Nie, Jukui and Lian, Tengfei and Jin, Jianshi and Liljas, Anders and Su, Xiao Dong},
  issn         = {2045-2322},
  language     = {eng},
  month        = {07},
  publisher    = {Nature Publishing Group},
  series       = {Scientific Reports},
  title        = {Deep sequencing reveals global patterns of mRNA recruitment during translation initiation},
  url          = {http://dx.doi.org/10.1038/srep30170},
  volume       = {6},
  year         = {2016},
}