Preparation of a universally usable, animal product free, defined medium for 2D and 3D culturing of normal and cancer cells
(2024) In MethodsX 12.- Abstract
Since 1958, cell culture media supplemented with fetal bovine serum is used, despite the well-known concerns about animal welfare, reproducibility, reliability, relevance, and safety. To obliterate these concerns and increase scientific accuracy, we recently published an open access, publicly available paper on a defined medium composition to make it possible for any lab to prepare this medium. The medium supports routine culturing and cell banking as well as investigations of growth curves, dose response testing of compounds of cells in 2D and 3D, and cell migration; all important aspects for research and toxicology. Here we give a detailed description of how to mix the defined universal cell culture medium in 14 simple steps to... (More)
Since 1958, cell culture media supplemented with fetal bovine serum is used, despite the well-known concerns about animal welfare, reproducibility, reliability, relevance, and safety. To obliterate these concerns and increase scientific accuracy, we recently published an open access, publicly available paper on a defined medium composition to make it possible for any lab to prepare this medium. The medium supports routine culturing and cell banking as well as investigations of growth curves, dose response testing of compounds of cells in 2D and 3D, and cell migration; all important aspects for research and toxicology. Here we give a detailed description of how to mix the defined universal cell culture medium in 14 simple steps to support any entity that wishes to make it. We also list different normal and cancer cell lines that have been cultured in the defined medium.
(Less)
- author
- Weber, Tilo ; Bajramovic, Jeffrey and Oredsson, Stina LU
- organization
- publishing date
- 2024-06
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Animal free medium, Cell culture, Defined medium, Ethically acceptable, Human proteins, Reproducible composition
- in
- MethodsX
- volume
- 12
- article number
- 102592
- publisher
- Elsevier
- external identifiers
-
- scopus:85187680432
- pmid:38445175
- ISSN
- 2215-0161
- DOI
- 10.1016/j.mex.2024.102592
- language
- English
- LU publication?
- yes
- id
- 372f435a-c94b-4181-8053-8abfa6f3b39e
- date added to LUP
- 2024-04-04 13:17:16
- date last changed
- 2024-06-13 20:44:34
@article{372f435a-c94b-4181-8053-8abfa6f3b39e,
abstract = {{<p>Since 1958, cell culture media supplemented with fetal bovine serum is used, despite the well-known concerns about animal welfare, reproducibility, reliability, relevance, and safety. To obliterate these concerns and increase scientific accuracy, we recently published an open access, publicly available paper on a defined medium composition to make it possible for any lab to prepare this medium. The medium supports routine culturing and cell banking as well as investigations of growth curves, dose response testing of compounds of cells in 2D and 3D, and cell migration; all important aspects for research and toxicology. Here we give a detailed description of how to mix the defined universal cell culture medium in 14 simple steps to support any entity that wishes to make it. We also list different normal and cancer cell lines that have been cultured in the defined medium.</p>}},
author = {{Weber, Tilo and Bajramovic, Jeffrey and Oredsson, Stina}},
issn = {{2215-0161}},
keywords = {{Animal free medium; Cell culture; Defined medium; Ethically acceptable; Human proteins; Reproducible composition}},
language = {{eng}},
publisher = {{Elsevier}},
series = {{MethodsX}},
title = {{Preparation of a universally usable, animal product free, defined medium for 2D and 3D culturing of normal and cancer cells}},
url = {{http://dx.doi.org/10.1016/j.mex.2024.102592}},
doi = {{10.1016/j.mex.2024.102592}},
volume = {{12}},
year = {{2024}},
}