Advanced

Isolation, characterization and multiplex polymerase chain reaction of novel microsatellite loci for the avian parasite Philornis downsi (Diptera: Muscidae)

Dudaniec, Rachael LU ; Gardner, Michael G. and Kleindorfer, Sonia (2008) In Molecular Ecology Resources 8(1). p.142-144
Abstract
An enrichment technique was used to isolate 11 di-, tri-, and tetra microsatellites for the parasitic fly Philornis downsi (Diptera: Muscidae). These loci were polymerase chain reaction amplified in singleplexes or two-plexes for P. downsi. The loci showed low to moderate polymorphism, exhibited between three and four alleles, and observed heterozygosity ranged from 0.05 to 0.86. These new markers will be useful for population-level and paternity analyses and will provide valuable information about the ecology of this high-impact parasite of vulnerable bird species.
Please use this url to cite or link to this publication:
author
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Darwin's finches, ectoparasitism, microsatellites, multiplex PCR, paternity, Philornis
in
Molecular Ecology Resources
volume
8
issue
1
pages
142 - 144
publisher
Wiley-Blackwell
external identifiers
  • scopus:36849030765
ISSN
1755-098X
DOI
10.1111/j.1471-8286.2007.01900.x
language
English
LU publication?
no
id
73907bd5-7eb9-4e0c-889a-4bfbad3baec2 (old id 3738422)
date added to LUP
2013-05-23 13:23:12
date last changed
2017-01-01 05:14:54
@article{73907bd5-7eb9-4e0c-889a-4bfbad3baec2,
  abstract     = {An enrichment technique was used to isolate 11 di-, tri-, and tetra microsatellites for the parasitic fly Philornis downsi (Diptera: Muscidae). These loci were polymerase chain reaction amplified in singleplexes or two-plexes for P. downsi. The loci showed low to moderate polymorphism, exhibited between three and four alleles, and observed heterozygosity ranged from 0.05 to 0.86. These new markers will be useful for population-level and paternity analyses and will provide valuable information about the ecology of this high-impact parasite of vulnerable bird species.},
  author       = {Dudaniec, Rachael and Gardner, Michael G. and Kleindorfer, Sonia},
  issn         = {1755-098X},
  keyword      = {Darwin's finches,ectoparasitism,microsatellites,multiplex PCR,paternity,Philornis},
  language     = {eng},
  number       = {1},
  pages        = {142--144},
  publisher    = {Wiley-Blackwell},
  series       = {Molecular Ecology Resources},
  title        = {Isolation, characterization and multiplex polymerase chain reaction of novel microsatellite loci for the avian parasite Philornis downsi (Diptera: Muscidae)},
  url          = {http://dx.doi.org/10.1111/j.1471-8286.2007.01900.x},
  volume       = {8},
  year         = {2008},
}