Tiliqua rugosa microsatellites: isolation via enrichment and characterisation of loci for multiplex PCR in T. rugosa and the endangered T. adelaidensis
(2008) In Conservation Genetics 9(1). p.233-237- Abstract
- We used an enrichment technique to isolate 18 novel di and tri microsatellites for the socially monogamous lizard Tiliqua rugosa. These loci were amplified in conjunction with previously described loci in two and three PCR multiplexes for T. rugosa and the endangered T. adelaidensis, respectively. The loci were highly polymorphic in both species, exhibiting between 2 and 32 alleles with observed heterozygosity ranging from 0.43 to 0.96. These markers will be useful for population-level analyses and can contribute to a genetic foundation for conservation strategies for the endangered T. adelaidensis.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3738476
- author
- Gardner, Michael G ; Sanchez, Juan J ; Dudaniec, Rachael LU ; Rheinberger, Leah ; Smith, Annabel L and Saint, Kathleen M
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Sociality, Microsatellite enrichment, Egernia, Tiliqua, Multiplex PCR, Monogamy
- in
- Conservation Genetics
- volume
- 9
- issue
- 1
- pages
- 233 - 237
- publisher
- Springer
- external identifiers
-
- scopus:38049088375
- ISSN
- 1566-0621
- DOI
- 10.1007/s10592-007-9316-0
- language
- English
- LU publication?
- no
- additional info
- 1
- id
- 8e02669b-e136-4365-b538-035e013ee43d (old id 3738476)
- date added to LUP
- 2016-04-01 12:14:28
- date last changed
- 2022-04-05 19:28:08
@article{8e02669b-e136-4365-b538-035e013ee43d, abstract = {{We used an enrichment technique to isolate 18 novel di and tri microsatellites for the socially monogamous lizard Tiliqua rugosa. These loci were amplified in conjunction with previously described loci in two and three PCR multiplexes for T. rugosa and the endangered T. adelaidensis, respectively. The loci were highly polymorphic in both species, exhibiting between 2 and 32 alleles with observed heterozygosity ranging from 0.43 to 0.96. These markers will be useful for population-level analyses and can contribute to a genetic foundation for conservation strategies for the endangered T. adelaidensis.}}, author = {{Gardner, Michael G and Sanchez, Juan J and Dudaniec, Rachael and Rheinberger, Leah and Smith, Annabel L and Saint, Kathleen M}}, issn = {{1566-0621}}, keywords = {{Sociality; Microsatellite enrichment; Egernia; Tiliqua; Multiplex PCR; Monogamy}}, language = {{eng}}, number = {{1}}, pages = {{233--237}}, publisher = {{Springer}}, series = {{Conservation Genetics}}, title = {{Tiliqua rugosa microsatellites: isolation via enrichment and characterisation of loci for multiplex PCR in T. rugosa and the endangered T. adelaidensis}}, url = {{http://dx.doi.org/10.1007/s10592-007-9316-0}}, doi = {{10.1007/s10592-007-9316-0}}, volume = {{9}}, year = {{2008}}, }