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Tiliqua rugosa microsatellites: isolation via enrichment and characterisation of loci for multiplex PCR in T. rugosa and the endangered T. adelaidensis

Gardner, Michael G; Sanchez, Juan J; Dudaniec, Rachael LU ; Rheinberger, Leah; Smith, Annabel L and Saint, Kathleen M (2008) In Conservation Genetics 9(1). p.233-237
Abstract
We used an enrichment technique to isolate 18 novel di and tri microsatellites for the socially monogamous lizard Tiliqua rugosa. These loci were amplified in conjunction with previously described loci in two and three PCR multiplexes for T. rugosa and the endangered T. adelaidensis, respectively. The loci were highly polymorphic in both species, exhibiting between 2 and 32 alleles with observed heterozygosity ranging from 0.43 to 0.96. These markers will be useful for population-level analyses and can contribute to a genetic foundation for conservation strategies for the endangered T. adelaidensis.
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author
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Sociality, Microsatellite enrichment, Egernia, Tiliqua, Multiplex PCR, Monogamy
in
Conservation Genetics
volume
9
issue
1
pages
233 - 237
publisher
Springer
external identifiers
  • scopus:38049088375
ISSN
1566-0621
DOI
10.1007/s10592-007-9316-0
language
English
LU publication?
no
id
8e02669b-e136-4365-b538-035e013ee43d (old id 3738476)
date added to LUP
2013-05-23 13:16:52
date last changed
2017-01-01 04:58:05
@article{8e02669b-e136-4365-b538-035e013ee43d,
  abstract     = {We used an enrichment technique to isolate 18 novel di and tri microsatellites for the socially monogamous lizard Tiliqua rugosa. These loci were amplified in conjunction with previously described loci in two and three PCR multiplexes for T. rugosa and the endangered T. adelaidensis, respectively. The loci were highly polymorphic in both species, exhibiting between 2 and 32 alleles with observed heterozygosity ranging from 0.43 to 0.96. These markers will be useful for population-level analyses and can contribute to a genetic foundation for conservation strategies for the endangered T. adelaidensis.},
  author       = {Gardner, Michael G and Sanchez, Juan J and Dudaniec, Rachael and Rheinberger, Leah and Smith, Annabel L and Saint, Kathleen M},
  issn         = {1566-0621},
  keyword      = {Sociality,Microsatellite enrichment,Egernia,Tiliqua,Multiplex PCR,Monogamy},
  language     = {eng},
  number       = {1},
  pages        = {233--237},
  publisher    = {Springer},
  series       = {Conservation Genetics},
  title        = {Tiliqua rugosa microsatellites: isolation via enrichment and characterisation of loci for multiplex PCR in T. rugosa and the endangered T. adelaidensis},
  url          = {http://dx.doi.org/10.1007/s10592-007-9316-0},
  volume       = {9},
  year         = {2008},
}