Fast on-column protein digestion with subsequent peptide mapping using tandem mass spectrometry with information dependent acquisition
(2005) In Journal of Chromatography A 1080(2). p.117-123- Abstract
- A platform for rapid on-line protein digestion of protein mixtures for direct infusion to a mass spectrometer is presented. A mixture of protein A, staphylococcal enterotoxin B and cytochrome c was used as a model mixture injected on a gel filtration column and a trypsin reactor which were connected in series to a micro liquid chromatography (mu LC) system. The peptides in the column eluate were analyzed with ESI tandem mass spectrometry, utilizing information dependent acquisition (IDA). In one step, the proteins in the mixture (mu M concentrations) were concomitantly desalted, separated, digested and identified with an overall analysis time of less than 40 min. Protein sequence coverage of 78-95% for the involved substances was achieved.... (More)
- A platform for rapid on-line protein digestion of protein mixtures for direct infusion to a mass spectrometer is presented. A mixture of protein A, staphylococcal enterotoxin B and cytochrome c was used as a model mixture injected on a gel filtration column and a trypsin reactor which were connected in series to a micro liquid chromatography (mu LC) system. The peptides in the column eluate were analyzed with ESI tandem mass spectrometry, utilizing information dependent acquisition (IDA). In one step, the proteins in the mixture (mu M concentrations) were concomitantly desalted, separated, digested and identified with an overall analysis time of less than 40 min. Protein sequence coverage of 78-95% for the involved substances was achieved. (c) 2005 Elsevier B.V. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/155109
- author
- Hedström, Martin LU ; Andersson, Maria LU ; Galaev, Igor LU and Mattiasson, Bo LU
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Chromatography A
- volume
- 1080
- issue
- 2
- pages
- 117 - 123
- publisher
- Elsevier
- external identifiers
-
- wos:000230238600003
- pmid:16008049
- scopus:20444484810
- ISSN
- 0021-9673
- DOI
- 10.1016/j.chroma.2005.04.069
- language
- English
- LU publication?
- yes
- id
- 3738f58f-c701-41d2-b1c0-1c7282f3ac63 (old id 155109)
- date added to LUP
- 2016-04-01 15:47:59
- date last changed
- 2022-01-28 07:11:23
@article{3738f58f-c701-41d2-b1c0-1c7282f3ac63, abstract = {{A platform for rapid on-line protein digestion of protein mixtures for direct infusion to a mass spectrometer is presented. A mixture of protein A, staphylococcal enterotoxin B and cytochrome c was used as a model mixture injected on a gel filtration column and a trypsin reactor which were connected in series to a micro liquid chromatography (mu LC) system. The peptides in the column eluate were analyzed with ESI tandem mass spectrometry, utilizing information dependent acquisition (IDA). In one step, the proteins in the mixture (mu M concentrations) were concomitantly desalted, separated, digested and identified with an overall analysis time of less than 40 min. Protein sequence coverage of 78-95% for the involved substances was achieved. (c) 2005 Elsevier B.V. All rights reserved.}}, author = {{Hedström, Martin and Andersson, Maria and Galaev, Igor and Mattiasson, Bo}}, issn = {{0021-9673}}, language = {{eng}}, number = {{2}}, pages = {{117--123}}, publisher = {{Elsevier}}, series = {{Journal of Chromatography A}}, title = {{Fast on-column protein digestion with subsequent peptide mapping using tandem mass spectrometry with information dependent acquisition}}, url = {{http://dx.doi.org/10.1016/j.chroma.2005.04.069}}, doi = {{10.1016/j.chroma.2005.04.069}}, volume = {{1080}}, year = {{2005}}, }