Femtosecond time-resolved absorption spectroscopy of astaxanthin in solution and in alpha-crustacyanin
(2005) In The Journal of Physical Chemistry Part A: Molecules, Spectroscopy, Kinetics, Environment and General Theory 109(14). p.3120-3127- Abstract
- Steady-state absorption and femtosecond time-resolved spectroscopic studies have been carried out on astaxanthin dissolved in CS2, methanol, and acetonitrile, and in purified alpha-crustacyanin. The spectra of the S-0 -> S-2 and S-1 -> S-n transitions were found to be similarly dependent on solvent environment. The dynamics of the excited-state decay processes were analyzed with both single wavelength and global fitting procedures. In solution, the S-1 lifetime of astaxanthin was found to be similar to 5 ps and independent of solvent. In alpha-crustacyanin, the lifetime was noticeably shorter at similar to 1.8 ps. Both fitting procedures led to the conclusion that the lifetime of the S-2 state was either comparable to or shorter than... (More)
- Steady-state absorption and femtosecond time-resolved spectroscopic studies have been carried out on astaxanthin dissolved in CS2, methanol, and acetonitrile, and in purified alpha-crustacyanin. The spectra of the S-0 -> S-2 and S-1 -> S-n transitions were found to be similarly dependent on solvent environment. The dynamics of the excited-state decay processes were analyzed with both single wavelength and global fitting procedures. In solution, the S-1 lifetime of astaxanthin was found to be similar to 5 ps and independent of solvent. In alpha-crustacyanin, the lifetime was noticeably shorter at similar to 1.8 ps. Both fitting procedures led to the conclusion that the lifetime of the S-2 state was either comparable to or shorter than the instrument response time. The data support the idea that dimerization of astaxanthin in alpha-crustacyanin is the primary molecular basis for the bathochromic shift of the S-0 -> S-2 and S-1 - S-n transitions. Planarization of the astaxanthin molecule, which leads to a longer effective pi-electron conjugated chain and a lower S-1 energy, accounts for the shorter tau(1) in the protein. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/151945
- author
- Ilagan, R P ; Christensen, R L ; Chapp, T W ; Gibson, G N ; Pascher, Torbjörn LU ; Polivka, Tomas LU and Frank, H A
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- in
- The Journal of Physical Chemistry Part A: Molecules, Spectroscopy, Kinetics, Environment and General Theory
- volume
- 109
- issue
- 14
- pages
- 3120 - 3127
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- wos:000228230800004
- scopus:17444375694
- pmid:16833638
- ISSN
- 1520-5215
- DOI
- 10.1021/jp0444161
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Chemical Physics (S) (011001060)
- id
- 3741f260-a880-4900-8b9e-fd193c5ac998 (old id 151945)
- date added to LUP
- 2016-04-01 17:14:13
- date last changed
- 2022-02-13 03:42:13
@article{3741f260-a880-4900-8b9e-fd193c5ac998,
abstract = {{Steady-state absorption and femtosecond time-resolved spectroscopic studies have been carried out on astaxanthin dissolved in CS2, methanol, and acetonitrile, and in purified alpha-crustacyanin. The spectra of the S-0 -> S-2 and S-1 -> S-n transitions were found to be similarly dependent on solvent environment. The dynamics of the excited-state decay processes were analyzed with both single wavelength and global fitting procedures. In solution, the S-1 lifetime of astaxanthin was found to be similar to 5 ps and independent of solvent. In alpha-crustacyanin, the lifetime was noticeably shorter at similar to 1.8 ps. Both fitting procedures led to the conclusion that the lifetime of the S-2 state was either comparable to or shorter than the instrument response time. The data support the idea that dimerization of astaxanthin in alpha-crustacyanin is the primary molecular basis for the bathochromic shift of the S-0 -> S-2 and S-1 - S-n transitions. Planarization of the astaxanthin molecule, which leads to a longer effective pi-electron conjugated chain and a lower S-1 energy, accounts for the shorter tau(1) in the protein.}},
author = {{Ilagan, R P and Christensen, R L and Chapp, T W and Gibson, G N and Pascher, Torbjörn and Polivka, Tomas and Frank, H A}},
issn = {{1520-5215}},
language = {{eng}},
number = {{14}},
pages = {{3120--3127}},
publisher = {{The American Chemical Society (ACS)}},
series = {{The Journal of Physical Chemistry Part A: Molecules, Spectroscopy, Kinetics, Environment and General Theory}},
title = {{Femtosecond time-resolved absorption spectroscopy of astaxanthin in solution and in alpha-crustacyanin}},
url = {{http://dx.doi.org/10.1021/jp0444161}},
doi = {{10.1021/jp0444161}},
volume = {{109}},
year = {{2005}},
}