Binding of antibodies to concanavalin A-modified monolithic cryogel
(2006) In Reactive & Functional Polymers 66(11). p.1263-1271- Abstract
- Binding of human immunoglobulin-G (IgG) from aqueous solutions and human plasma to concanavalin A (Con A) immobilized poly(acrylamide-allyl glycidyl ether) [poly(AAm-AGE)] monolithic cryogel has been studied. Poly(AAm-AGE) cryogel was prepared by bulk polymerization which proceeds in aqueous solution of monomers frozen inside a column (cryo-polymerization). After thawing, the monolithic cryogel contains a continuous polymeric matrix having interconnected pores of 10-100 pm size. Con A was immobilized by covalent binding onto poly(AAm-AGE) cryogel via epoxy groups. The maximum IgG adsorption on the Con A-poly(AAm-AGE) cryogels was observed at pH 7.4 from aqueous solutions. The non-specific IgG adsorption onto the plain poly(AAm-AGE)... (More)
- Binding of human immunoglobulin-G (IgG) from aqueous solutions and human plasma to concanavalin A (Con A) immobilized poly(acrylamide-allyl glycidyl ether) [poly(AAm-AGE)] monolithic cryogel has been studied. Poly(AAm-AGE) cryogel was prepared by bulk polymerization which proceeds in aqueous solution of monomers frozen inside a column (cryo-polymerization). After thawing, the monolithic cryogel contains a continuous polymeric matrix having interconnected pores of 10-100 pm size. Con A was immobilized by covalent binding onto poly(AAm-AGE) cryogel via epoxy groups. The maximum IgG adsorption on the Con A-poly(AAm-AGE) cryogels was observed at pH 7.4 from aqueous solutions. The non-specific IgG adsorption onto the plain poly(AAm-AGE) adsorbents was about 0.25 mg/g. Up to 6.7 mg/g IgG were bound to Con A-poly(AAm-AGE) cryogels from aqueous solutions. The large pore size of the cryogel makes it possible to process blood cells without blocking the column. Higher adsorption capacity was observed from human plasma (up to 25.6 mg/g). Bound IgG was eluted using 2.0 M NaCl with a purity of 85%. Adsorption capacities of other blood proteins were obtained as 1.0 mg/g for fibrinogen and 1.7 mg/g for albumin. The total protein adsorption was determined as 28.6 mg/g. Con A-poly(AAm-AGE) cryogels was used for repetitive adsorption/desorption of IgG molecules without noticeable loss in IgG adsorption capacity after 10 cycles. (c) 2006 Elsevier B.V. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/376488
- author
- Babac, Ceyhun ; Yavuz, Handan ; Galaev, Igor LU ; Piskin, Erhan and Denizli, Adil
- organization
- publishing date
- 2006
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- IgG, antibody purification, cryogels, monoliths, concanavalin A
- in
- Reactive & Functional Polymers
- volume
- 66
- issue
- 11
- pages
- 1263 - 1271
- publisher
- Elsevier
- external identifiers
-
- wos:000242231400007
- scopus:33748766609
- ISSN
- 1873-166X
- DOI
- 10.1016/j.reactfunctpolym.2006.03.007
- language
- English
- LU publication?
- yes
- id
- e3694ae0-a51d-4853-9544-c655e9c37a4a (old id 376488)
- date added to LUP
- 2016-04-01 12:22:26
- date last changed
- 2022-01-27 02:54:09
@article{e3694ae0-a51d-4853-9544-c655e9c37a4a,
abstract = {{Binding of human immunoglobulin-G (IgG) from aqueous solutions and human plasma to concanavalin A (Con A) immobilized poly(acrylamide-allyl glycidyl ether) [poly(AAm-AGE)] monolithic cryogel has been studied. Poly(AAm-AGE) cryogel was prepared by bulk polymerization which proceeds in aqueous solution of monomers frozen inside a column (cryo-polymerization). After thawing, the monolithic cryogel contains a continuous polymeric matrix having interconnected pores of 10-100 pm size. Con A was immobilized by covalent binding onto poly(AAm-AGE) cryogel via epoxy groups. The maximum IgG adsorption on the Con A-poly(AAm-AGE) cryogels was observed at pH 7.4 from aqueous solutions. The non-specific IgG adsorption onto the plain poly(AAm-AGE) adsorbents was about 0.25 mg/g. Up to 6.7 mg/g IgG were bound to Con A-poly(AAm-AGE) cryogels from aqueous solutions. The large pore size of the cryogel makes it possible to process blood cells without blocking the column. Higher adsorption capacity was observed from human plasma (up to 25.6 mg/g). Bound IgG was eluted using 2.0 M NaCl with a purity of 85%. Adsorption capacities of other blood proteins were obtained as 1.0 mg/g for fibrinogen and 1.7 mg/g for albumin. The total protein adsorption was determined as 28.6 mg/g. Con A-poly(AAm-AGE) cryogels was used for repetitive adsorption/desorption of IgG molecules without noticeable loss in IgG adsorption capacity after 10 cycles. (c) 2006 Elsevier B.V. All rights reserved.}},
author = {{Babac, Ceyhun and Yavuz, Handan and Galaev, Igor and Piskin, Erhan and Denizli, Adil}},
issn = {{1873-166X}},
keywords = {{IgG; antibody purification; cryogels; monoliths; concanavalin A}},
language = {{eng}},
number = {{11}},
pages = {{1263--1271}},
publisher = {{Elsevier}},
series = {{Reactive & Functional Polymers}},
title = {{Binding of antibodies to concanavalin A-modified monolithic cryogel}},
url = {{http://dx.doi.org/10.1016/j.reactfunctpolym.2006.03.007}},
doi = {{10.1016/j.reactfunctpolym.2006.03.007}},
volume = {{66}},
year = {{2006}},
}