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Binding of antibodies to concanavalin A-modified monolithic cryogel

Babac, Ceyhun ; Yavuz, Handan ; Galaev, Igor LU ; Piskin, Erhan and Denizli, Adil (2006) In Reactive & Functional Polymers 66(11). p.1263-1271
Abstract
Binding of human immunoglobulin-G (IgG) from aqueous solutions and human plasma to concanavalin A (Con A) immobilized poly(acrylamide-allyl glycidyl ether) [poly(AAm-AGE)] monolithic cryogel has been studied. Poly(AAm-AGE) cryogel was prepared by bulk polymerization which proceeds in aqueous solution of monomers frozen inside a column (cryo-polymerization). After thawing, the monolithic cryogel contains a continuous polymeric matrix having interconnected pores of 10-100 pm size. Con A was immobilized by covalent binding onto poly(AAm-AGE) cryogel via epoxy groups. The maximum IgG adsorption on the Con A-poly(AAm-AGE) cryogels was observed at pH 7.4 from aqueous solutions. The non-specific IgG adsorption onto the plain poly(AAm-AGE)... (More)
Binding of human immunoglobulin-G (IgG) from aqueous solutions and human plasma to concanavalin A (Con A) immobilized poly(acrylamide-allyl glycidyl ether) [poly(AAm-AGE)] monolithic cryogel has been studied. Poly(AAm-AGE) cryogel was prepared by bulk polymerization which proceeds in aqueous solution of monomers frozen inside a column (cryo-polymerization). After thawing, the monolithic cryogel contains a continuous polymeric matrix having interconnected pores of 10-100 pm size. Con A was immobilized by covalent binding onto poly(AAm-AGE) cryogel via epoxy groups. The maximum IgG adsorption on the Con A-poly(AAm-AGE) cryogels was observed at pH 7.4 from aqueous solutions. The non-specific IgG adsorption onto the plain poly(AAm-AGE) adsorbents was about 0.25 mg/g. Up to 6.7 mg/g IgG were bound to Con A-poly(AAm-AGE) cryogels from aqueous solutions. The large pore size of the cryogel makes it possible to process blood cells without blocking the column. Higher adsorption capacity was observed from human plasma (up to 25.6 mg/g). Bound IgG was eluted using 2.0 M NaCl with a purity of 85%. Adsorption capacities of other blood proteins were obtained as 1.0 mg/g for fibrinogen and 1.7 mg/g for albumin. The total protein adsorption was determined as 28.6 mg/g. Con A-poly(AAm-AGE) cryogels was used for repetitive adsorption/desorption of IgG molecules without noticeable loss in IgG adsorption capacity after 10 cycles. (c) 2006 Elsevier B.V. All rights reserved. (Less)
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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
IgG, antibody purification, cryogels, monoliths, concanavalin A
in
Reactive & Functional Polymers
volume
66
issue
11
pages
1263 - 1271
publisher
Elsevier
external identifiers
  • wos:000242231400007
  • scopus:33748766609
ISSN
1873-166X
DOI
10.1016/j.reactfunctpolym.2006.03.007
language
English
LU publication?
yes
id
e3694ae0-a51d-4853-9544-c655e9c37a4a (old id 376488)
date added to LUP
2016-04-01 12:22:26
date last changed
2021-09-15 03:23:22
@article{e3694ae0-a51d-4853-9544-c655e9c37a4a,
  abstract     = {Binding of human immunoglobulin-G (IgG) from aqueous solutions and human plasma to concanavalin A (Con A) immobilized poly(acrylamide-allyl glycidyl ether) [poly(AAm-AGE)] monolithic cryogel has been studied. Poly(AAm-AGE) cryogel was prepared by bulk polymerization which proceeds in aqueous solution of monomers frozen inside a column (cryo-polymerization). After thawing, the monolithic cryogel contains a continuous polymeric matrix having interconnected pores of 10-100 pm size. Con A was immobilized by covalent binding onto poly(AAm-AGE) cryogel via epoxy groups. The maximum IgG adsorption on the Con A-poly(AAm-AGE) cryogels was observed at pH 7.4 from aqueous solutions. The non-specific IgG adsorption onto the plain poly(AAm-AGE) adsorbents was about 0.25 mg/g. Up to 6.7 mg/g IgG were bound to Con A-poly(AAm-AGE) cryogels from aqueous solutions. The large pore size of the cryogel makes it possible to process blood cells without blocking the column. Higher adsorption capacity was observed from human plasma (up to 25.6 mg/g). Bound IgG was eluted using 2.0 M NaCl with a purity of 85%. Adsorption capacities of other blood proteins were obtained as 1.0 mg/g for fibrinogen and 1.7 mg/g for albumin. The total protein adsorption was determined as 28.6 mg/g. Con A-poly(AAm-AGE) cryogels was used for repetitive adsorption/desorption of IgG molecules without noticeable loss in IgG adsorption capacity after 10 cycles. (c) 2006 Elsevier B.V. All rights reserved.},
  author       = {Babac, Ceyhun and Yavuz, Handan and Galaev, Igor and Piskin, Erhan and Denizli, Adil},
  issn         = {1873-166X},
  language     = {eng},
  number       = {11},
  pages        = {1263--1271},
  publisher    = {Elsevier},
  series       = {Reactive & Functional Polymers},
  title        = {Binding of antibodies to concanavalin A-modified monolithic cryogel},
  url          = {http://dx.doi.org/10.1016/j.reactfunctpolym.2006.03.007},
  doi          = {10.1016/j.reactfunctpolym.2006.03.007},
  volume       = {66},
  year         = {2006},
}