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Identification and characterization of secretagogin promoter activity

Skovhus, K. V. ; Bergholdt, R ; Erichsen, C ; Sparre, T. ; Nerup, Jörn LU ; Karlsen, A. E. and Pociot, F. (2006) In Scandinavian Journal of Immunology 64(6). p.639-645
Abstract
Secretagogin is a newly identified calcium-binding protein selectively expressed in neuroendocrine tissue and pancreatic beta-cells. The function of secretagogin is unknown, but it has been suggested in beta-cells to influence calcium-influx, insulin secretion and proliferation, and has been observed downregulated in diabetes-prone BB rat islets exposed to cytokines. In the present study, we identified and characterized promoter activity of a human 1498 bp sequence upstream the transcription start site. The promoter sequence showed subtle but significant regulation by glucose within the normo-physiological range. Glucose also led to changes in expression of secretagogin protein in INS-1e cells, but not in primary cells from... (More)
Secretagogin is a newly identified calcium-binding protein selectively expressed in neuroendocrine tissue and pancreatic beta-cells. The function of secretagogin is unknown, but it has been suggested in beta-cells to influence calcium-influx, insulin secretion and proliferation, and has been observed downregulated in diabetes-prone BB rat islets exposed to cytokines. In the present study, we identified and characterized promoter activity of a human 1498 bp sequence upstream the transcription start site. The promoter sequence showed subtle but significant regulation by glucose within the normo-physiological range. Glucose also led to changes in expression of secretagogin protein in INS-1e cells, but not in primary cells from non-diabetes-prone Wistar Furth rats. No effects of cytokines neither on promoter activity nor protein expression were observed. The promoter region was furthermore screened by direct sequencing, and 11 polymorphisms were identified. Genotyping in a large homogenous Type 1 diabetes (T1D) family collection did not reveal association with T1D. (Less)
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Scandinavian Journal of Immunology
volume
64
issue
6
pages
639 - 645
publisher
Wiley-Blackwell
external identifiers
  • wos:000241776300008
  • scopus:33750700761
ISSN
1365-3083
DOI
10.1111/j.1365-3083.2006.01854.x
language
English
LU publication?
yes
id
870595f7-f00a-4a1b-a50a-16e14ce941aa (old id 377335)
date added to LUP
2016-04-01 17:05:41
date last changed
2021-05-25 03:13:24
@article{870595f7-f00a-4a1b-a50a-16e14ce941aa,
  abstract     = {Secretagogin is a newly identified calcium-binding protein selectively expressed in neuroendocrine tissue and pancreatic beta-cells. The function of secretagogin is unknown, but it has been suggested in beta-cells to influence calcium-influx, insulin secretion and proliferation, and has been observed downregulated in diabetes-prone BB rat islets exposed to cytokines. In the present study, we identified and characterized promoter activity of a human 1498 bp sequence upstream the transcription start site. The promoter sequence showed subtle but significant regulation by glucose within the normo-physiological range. Glucose also led to changes in expression of secretagogin protein in INS-1e cells, but not in primary cells from non-diabetes-prone Wistar Furth rats. No effects of cytokines neither on promoter activity nor protein expression were observed. The promoter region was furthermore screened by direct sequencing, and 11 polymorphisms were identified. Genotyping in a large homogenous Type 1 diabetes (T1D) family collection did not reveal association with T1D.},
  author       = {Skovhus, K. V. and Bergholdt, R and Erichsen, C and Sparre, T. and Nerup, Jörn and Karlsen, A. E. and Pociot, F.},
  issn         = {1365-3083},
  language     = {eng},
  number       = {6},
  pages        = {639--645},
  publisher    = {Wiley-Blackwell},
  series       = {Scandinavian Journal of Immunology},
  title        = {Identification and characterization of secretagogin promoter activity},
  url          = {http://dx.doi.org/10.1111/j.1365-3083.2006.01854.x},
  doi          = {10.1111/j.1365-3083.2006.01854.x},
  volume       = {64},
  year         = {2006},
}