Role of epithelial interleukin-8 (IL-8) and neutrophil IL-8 receptor A in Escherichia coli-induced transuroepithelial neutrophil migration
Godaly, Gabriela LU
; Proudfoot, Amanda E
; Offord, Robin E
; Svanborg, Catharina
LU
and Agace, William W
(1997)
In Infection and Immunity
65(8).
p.3451-3456
- Abstract
- Escherichia coli stimulates neutrophil migration across human uroepithelial cell layers. This study investigated the role of the neutrophil chemokine interleukin-8 (IL-8) in this process. E. coli and IL-1alpha stimulated urinary tract epithelial layers to secrete IL-8 and induced transepithelial neutrophil migration. Anti-IL-8 antibody reduced neutrophil migration across epithelial cell layers, indicating a central role for this chemokine in the migration process. Furthermore, addition of recombinant IL-8 to unstimulated cell layers was sufficient to induce migration. The IL-8 dependence of neutrophil migration was maintained after removal of soluble IL-8 by washing of the cell layers. Flow cytometry analysis with fluorescein... (More)
- Escherichia coli stimulates neutrophil migration across human uroepithelial cell layers. This study investigated the role of the neutrophil chemokine interleukin-8 (IL-8) in this process. E. coli and IL-1alpha stimulated urinary tract epithelial layers to secrete IL-8 and induced transepithelial neutrophil migration. Anti-IL-8 antibody reduced neutrophil migration across epithelial cell layers, indicating a central role for this chemokine in the migration process. Furthermore, addition of recombinant IL-8 to unstimulated cell layers was sufficient to induce migration. The IL-8 dependence of neutrophil migration was maintained after removal of soluble IL-8 by washing of the cell layers. Flow cytometry analysis with fluorescein isothiocyanate-labelled IL-8 confirmed IL-8's ability to bind to the epithelial cell surface. Indirect immunofluorescence with confocal laser scanning microscopy showed that IL-8 associated with the epithelial cell layers. Prior incubation of neutrophils with antibodies to IL-8 receptor A (IL-8RA) reduced neutrophil migration. Anti-IL-8 RB antibody had no effect on neutrophil migration. These results demonstrate that IL-8 plays a key role in E. coli- or IL-1alpha-induced transuroepithelial migration and suggest that epithelial cell-produced IL-8 interacts with IL-8RA on the neutrophil surface. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1111992
- author
- Godaly, Gabriela
LU
; Proudfoot, Amanda E
; Offord, Robin E
; Svanborg, Catharina
LU
and Agace, William W
- organization
- publishing date
- 1997
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Infection and Immunity
- volume
- 65
- issue
- 8
- pages
- 3451 - 3456
- publisher
- American Society for Microbiology
- external identifiers
-
- pmid:9234811
- scopus:0030794069
- ISSN
- 1098-5522
- language
- English
- LU publication?
- yes
- id
- 3785625b-d627-483c-a1e8-74e847badc53 (old id 1111992)
- alternative location
- http://iai.highwire.org/cgi/reprint/65/8/3451
- date added to LUP
- 2016-04-01 11:42:19
- date last changed
- 2023-09-01 03:59:43
@article{3785625b-d627-483c-a1e8-74e847badc53,
abstract = {{Escherichia coli stimulates neutrophil migration across human uroepithelial cell layers. This study investigated the role of the neutrophil chemokine interleukin-8 (IL-8) in this process. E. coli and IL-1alpha stimulated urinary tract epithelial layers to secrete IL-8 and induced transepithelial neutrophil migration. Anti-IL-8 antibody reduced neutrophil migration across epithelial cell layers, indicating a central role for this chemokine in the migration process. Furthermore, addition of recombinant IL-8 to unstimulated cell layers was sufficient to induce migration. The IL-8 dependence of neutrophil migration was maintained after removal of soluble IL-8 by washing of the cell layers. Flow cytometry analysis with fluorescein isothiocyanate-labelled IL-8 confirmed IL-8's ability to bind to the epithelial cell surface. Indirect immunofluorescence with confocal laser scanning microscopy showed that IL-8 associated with the epithelial cell layers. Prior incubation of neutrophils with antibodies to IL-8 receptor A (IL-8RA) reduced neutrophil migration. Anti-IL-8 RB antibody had no effect on neutrophil migration. These results demonstrate that IL-8 plays a key role in E. coli- or IL-1alpha-induced transuroepithelial migration and suggest that epithelial cell-produced IL-8 interacts with IL-8RA on the neutrophil surface.}},
author = {{Godaly, Gabriela and Proudfoot, Amanda E and Offord, Robin E and Svanborg, Catharina and Agace, William W}},
issn = {{1098-5522}},
language = {{eng}},
number = {{8}},
pages = {{3451--3456}},
publisher = {{American Society for Microbiology}},
series = {{Infection and Immunity}},
title = {{Role of epithelial interleukin-8 (IL-8) and neutrophil IL-8 receptor A in Escherichia coli-induced transuroepithelial neutrophil migration}},
url = {{http://iai.highwire.org/cgi/reprint/65/8/3451}},
volume = {{65}},
year = {{1997}},
}