The cyclin-dependent kinase 8 module sterically blocks Mediator interactions with RNA polymerase II
(2006) In Proceedings of the National Academy of Sciences 103(43). p.15788-15793- Abstract
- CDK8 (cyclin-dependent kinase 8), along with CycC, Med12, and Med13, form a repressive module (the Cdk8 module) that prevents RNA polymerase II (pol II) interactions with Mediator. Here, we report that the ability of the Cdk8 module to prevent pol II interactions is independent of the Cdk8-dependent kinase activity. We use electron microscopy and single-particle reconstruction to demonstrate that the Cdk8 module forms a distinct structural entity that binds to the head and middle region of Mediator, thereby sterically blocking interactions with pol II.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/378610
- author
- Elmlund, Hans LU ; Baraznenok, Vera ; Lindahl, Martin LU ; Samuelsen, Camilla O. ; Koeck, Philip J. B. ; Holmberg, Steen ; Hebert, Hans and Gustafsson, Claes M.
- organization
- publishing date
- 2006
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- protein structure, electron microscopy, transcription, Schizosaccharomyces pombe, yeast
- in
- Proceedings of the National Academy of Sciences
- volume
- 103
- issue
- 43
- pages
- 15788 - 15793
- publisher
- National Academy of Sciences
- external identifiers
-
- wos:000241568500013
- scopus:33750481873
- pmid:17043218
- ISSN
- 1091-6490
- DOI
- 10.1073/pnas.0607483103
- language
- English
- LU publication?
- yes
- id
- 31b1ee7e-cf81-4b0e-8e72-bf4654a2ffc3 (old id 378610)
- date added to LUP
- 2016-04-01 12:13:35
- date last changed
- 2022-03-28 22:02:18
@article{31b1ee7e-cf81-4b0e-8e72-bf4654a2ffc3, abstract = {{CDK8 (cyclin-dependent kinase 8), along with CycC, Med12, and Med13, form a repressive module (the Cdk8 module) that prevents RNA polymerase II (pol II) interactions with Mediator. Here, we report that the ability of the Cdk8 module to prevent pol II interactions is independent of the Cdk8-dependent kinase activity. We use electron microscopy and single-particle reconstruction to demonstrate that the Cdk8 module forms a distinct structural entity that binds to the head and middle region of Mediator, thereby sterically blocking interactions with pol II.}}, author = {{Elmlund, Hans and Baraznenok, Vera and Lindahl, Martin and Samuelsen, Camilla O. and Koeck, Philip J. B. and Holmberg, Steen and Hebert, Hans and Gustafsson, Claes M.}}, issn = {{1091-6490}}, keywords = {{protein structure; electron microscopy; transcription; Schizosaccharomyces pombe; yeast}}, language = {{eng}}, number = {{43}}, pages = {{15788--15793}}, publisher = {{National Academy of Sciences}}, series = {{Proceedings of the National Academy of Sciences}}, title = {{The cyclin-dependent kinase 8 module sterically blocks Mediator interactions with RNA polymerase II}}, url = {{http://dx.doi.org/10.1073/pnas.0607483103}}, doi = {{10.1073/pnas.0607483103}}, volume = {{103}}, year = {{2006}}, }