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The cyclin-dependent kinase 8 module sterically blocks Mediator interactions with RNA polymerase II

Elmlund, Hans LU ; Baraznenok, Vera ; Lindahl, Martin LU ; Samuelsen, Camilla O. ; Koeck, Philip J. B. ; Holmberg, Steen ; Hebert, Hans and Gustafsson, Claes M. (2006) In Proceedings of the National Academy of Sciences 103(43). p.15788-15793
Abstract
CDK8 (cyclin-dependent kinase 8), along with CycC, Med12, and Med13, form a repressive module (the Cdk8 module) that prevents RNA polymerase II (pol II) interactions with Mediator. Here, we report that the ability of the Cdk8 module to prevent pol II interactions is independent of the Cdk8-dependent kinase activity. We use electron microscopy and single-particle reconstruction to demonstrate that the Cdk8 module forms a distinct structural entity that binds to the head and middle region of Mediator, thereby sterically blocking interactions with pol II.
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author
; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
protein structure, electron microscopy, transcription, Schizosaccharomyces pombe, yeast
in
Proceedings of the National Academy of Sciences
volume
103
issue
43
pages
15788 - 15793
publisher
National Acad Sciences
external identifiers
  • wos:000241568500013
  • scopus:33750481873
  • pmid:17043218
ISSN
1091-6490
DOI
10.1073/pnas.0607483103
language
English
LU publication?
yes
id
31b1ee7e-cf81-4b0e-8e72-bf4654a2ffc3 (old id 378610)
date added to LUP
2016-04-01 12:13:35
date last changed
2021-09-29 02:49:41
@article{31b1ee7e-cf81-4b0e-8e72-bf4654a2ffc3,
  abstract     = {CDK8 (cyclin-dependent kinase 8), along with CycC, Med12, and Med13, form a repressive module (the Cdk8 module) that prevents RNA polymerase II (pol II) interactions with Mediator. Here, we report that the ability of the Cdk8 module to prevent pol II interactions is independent of the Cdk8-dependent kinase activity. We use electron microscopy and single-particle reconstruction to demonstrate that the Cdk8 module forms a distinct structural entity that binds to the head and middle region of Mediator, thereby sterically blocking interactions with pol II.},
  author       = {Elmlund, Hans and Baraznenok, Vera and Lindahl, Martin and Samuelsen, Camilla O. and Koeck, Philip J. B. and Holmberg, Steen and Hebert, Hans and Gustafsson, Claes M.},
  issn         = {1091-6490},
  language     = {eng},
  number       = {43},
  pages        = {15788--15793},
  publisher    = {National Acad Sciences},
  series       = {Proceedings of the National Academy of Sciences},
  title        = {The cyclin-dependent kinase 8 module sterically blocks Mediator interactions with RNA polymerase II},
  url          = {http://dx.doi.org/10.1073/pnas.0607483103},
  doi          = {10.1073/pnas.0607483103},
  volume       = {103},
  year         = {2006},
}