Chondroitin sulfate perlecan enhances collagen fibril formation - Implications for perlecan chondrodysplasias
(2006) In Journal of Biological Chemistry 281(44). p.33127-33139- Abstract
- Inactivation of the perlecan gene leads to perinatal lethal chondrodysplasia. The similarity to the phenotypes of the Col2A1 knock-out and the disproportionate micromelia mutation suggests perlecan involvement in cartilage collagen matrix assembly. We now present a mechanism for the defect in collagen type II fibril assembly by perlecan-null chondrocytes. Cartilage perlecan is a heparin sulfate or a mixed heparan sulfate/ chondroitin sulfate proteoglycan. The latter form binds collagen and accelerates fibril formation in vitro, with more defined fibril morphology and increased fibril diameters produced in the presence of perlecan. Interestingly, the enhancement of collagen fibril formation is independent on the core protein and is mimicked... (More)
- Inactivation of the perlecan gene leads to perinatal lethal chondrodysplasia. The similarity to the phenotypes of the Col2A1 knock-out and the disproportionate micromelia mutation suggests perlecan involvement in cartilage collagen matrix assembly. We now present a mechanism for the defect in collagen type II fibril assembly by perlecan-null chondrocytes. Cartilage perlecan is a heparin sulfate or a mixed heparan sulfate/ chondroitin sulfate proteoglycan. The latter form binds collagen and accelerates fibril formation in vitro, with more defined fibril morphology and increased fibril diameters produced in the presence of perlecan. Interestingly, the enhancement of collagen fibril formation is independent on the core protein and is mimicked by chondroitin sulfate E but neither by chondroitin sulfate D nor dextran sulfate. Furthermore, perlecan chondroitin sulfate contains the 4,6-disulfated disaccharides typical for chondroitin sulfate E. Indeed, purified glycosaminoglycans from perlecan-enriched fractions of cartilage extracts contain elevated levels of 4,6-disulfated chondroitin sulfate disaccharides and enhance collagen fibril formation. The effect on collagen assembly is proportional to the content of the 4,6- disulfated disaccharide in the different cartilage extracts, with growth plate cartilage glycosaminoglycan being the most efficient enhancer. These findings demonstrate a role for perlecan chondroitin sulfate side chains in cartilage extracellular matrix assembly and provide an explanation for the perlecan-null chondrodysplasia. (Less)
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https://lup.lub.lu.se/record/378828
- author
- organization
- publishing date
- 2006
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Biological Chemistry
- volume
- 281
- issue
- 44
- pages
- 33127 - 33139
- publisher
- American Society for Biochemistry and Molecular Biology
- external identifiers
-
- wos:000241621400021
- scopus:33845947939
- ISSN
- 1083-351X
- DOI
- 10.1074/jbc.M607892200
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Department of Clinical Sciences, Lund (013230000), Department of Experimental Medical Science (013210000), Connective Tissue Biology (013230151), Division of Infection Medicine (BMC) (013024020), Pathology, (Lund) (013030000), Experimental Cardiovascular Research Unit (013242110)
- id
- 27c8c5e2-3886-48e4-8795-fddd71425321 (old id 378828)
- date added to LUP
- 2016-04-01 12:10:33
- date last changed
- 2022-03-21 00:33:51
@article{27c8c5e2-3886-48e4-8795-fddd71425321, abstract = {{Inactivation of the perlecan gene leads to perinatal lethal chondrodysplasia. The similarity to the phenotypes of the Col2A1 knock-out and the disproportionate micromelia mutation suggests perlecan involvement in cartilage collagen matrix assembly. We now present a mechanism for the defect in collagen type II fibril assembly by perlecan-null chondrocytes. Cartilage perlecan is a heparin sulfate or a mixed heparan sulfate/ chondroitin sulfate proteoglycan. The latter form binds collagen and accelerates fibril formation in vitro, with more defined fibril morphology and increased fibril diameters produced in the presence of perlecan. Interestingly, the enhancement of collagen fibril formation is independent on the core protein and is mimicked by chondroitin sulfate E but neither by chondroitin sulfate D nor dextran sulfate. Furthermore, perlecan chondroitin sulfate contains the 4,6-disulfated disaccharides typical for chondroitin sulfate E. Indeed, purified glycosaminoglycans from perlecan-enriched fractions of cartilage extracts contain elevated levels of 4,6-disulfated chondroitin sulfate disaccharides and enhance collagen fibril formation. The effect on collagen assembly is proportional to the content of the 4,6- disulfated disaccharide in the different cartilage extracts, with growth plate cartilage glycosaminoglycan being the most efficient enhancer. These findings demonstrate a role for perlecan chondroitin sulfate side chains in cartilage extracellular matrix assembly and provide an explanation for the perlecan-null chondrodysplasia.}}, author = {{Kvist, Alexander and Johnson, Anna and Mörgelin, Matthias and Gustafsson, Erika and Bengtsson, Eva and Lindblom, Karin and Aszodi, Attila and Faessler, Reinhard and Sasaki, Takako and Timpl, Rupert and Aspberg, Anders}}, issn = {{1083-351X}}, language = {{eng}}, number = {{44}}, pages = {{33127--33139}}, publisher = {{American Society for Biochemistry and Molecular Biology}}, series = {{Journal of Biological Chemistry}}, title = {{Chondroitin sulfate perlecan enhances collagen fibril formation - Implications for perlecan chondrodysplasias}}, url = {{http://dx.doi.org/10.1074/jbc.M607892200}}, doi = {{10.1074/jbc.M607892200}}, volume = {{281}}, year = {{2006}}, }