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Dual-focus coherent anti-Stokes Raman scattering microscopy using a compact two-beam fiber laser source

Legesse, Fisseha Bekele ; Meyer, Tobias ; Heuke, Sandro ; Gottschall, Thomas ; Pascher, Torbjörn LU ; Limpert, Jens ; Tünnermann, Andreas ; Schmitt, Michael and Popp, Jürgen (2017) In Optics Letters 42(2). p.183-186
Abstract

We have developed a dual-focus coherent anti-Stokes Raman scattering (CARS) microscope based on a dual output, compact fiber laser source. The underlying concepts of time-multiplexed, two-beam scanning and demultiplexed detection that we already employed for second-harmonic generation are here naturally extended for CARS microscopy. The layout of a robust, all-fiber laser source was reconfigured to provide two outputs, each containing the two colors necessary for the CARS process. The utilization of the design for simultaneously imaging two laterally or axially separated fields of view and, thus, inherently speeding up the image acquisition process, is demonstrated on human artery tissue samples.

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author
; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Optics Letters
volume
42
issue
2
pages
4 pages
publisher
Optical Society of America
external identifiers
  • scopus:85009476784
  • wos:000392193100003
  • pmid:28081075
ISSN
0146-9592
DOI
10.1364/OL.42.000183
language
English
LU publication?
yes
id
3792e004-0e70-40ba-b77b-866ef48feb7f
date added to LUP
2017-02-28 11:57:08
date last changed
2024-01-13 15:43:50
@article{3792e004-0e70-40ba-b77b-866ef48feb7f,
  abstract     = {{<p>We have developed a dual-focus coherent anti-Stokes Raman scattering (CARS) microscope based on a dual output, compact fiber laser source. The underlying concepts of time-multiplexed, two-beam scanning and demultiplexed detection that we already employed for second-harmonic generation are here naturally extended for CARS microscopy. The layout of a robust, all-fiber laser source was reconfigured to provide two outputs, each containing the two colors necessary for the CARS process. The utilization of the design for simultaneously imaging two laterally or axially separated fields of view and, thus, inherently speeding up the image acquisition process, is demonstrated on human artery tissue samples.</p>}},
  author       = {{Legesse, Fisseha Bekele and Meyer, Tobias and Heuke, Sandro and Gottschall, Thomas and Pascher, Torbjörn and Limpert, Jens and Tünnermann, Andreas and Schmitt, Michael and Popp, Jürgen}},
  issn         = {{0146-9592}},
  language     = {{eng}},
  month        = {{01}},
  number       = {{2}},
  pages        = {{183--186}},
  publisher    = {{Optical Society of America}},
  series       = {{Optics Letters}},
  title        = {{Dual-focus coherent anti-Stokes Raman scattering microscopy using a compact two-beam fiber laser source}},
  url          = {{http://dx.doi.org/10.1364/OL.42.000183}},
  doi          = {{10.1364/OL.42.000183}},
  volume       = {{42}},
  year         = {{2017}},
}