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Developmental plasticity in reptiles: Insights from temperature-dependent gene expression in wall lizard embryos : Insights from temperature-dependent gene expression in wall lizard embryos

Feiner, Nathalie LU ; Rago, Alfredo LU ; While, Geoffrey M and Uller, Tobias LU (2018) In Journal of experimental zoology. Part A, Ecological and integrative physiology 329(6-7). p.351-361
Abstract

Many features of the development of reptiles are affected by temperature, but very little is known about how incubation temperature affects gene expression. Here, we provide a detailed case study of gene expression profiles in common wall lizard (Podarcis muralis) embryos developing at stressfully low (15°C) versus benign (24°C) temperature. For maximum comparability between the two temperature regimes, we selected a precise developmental stage early in embryogenesis defined by the number of somites. We used a split-clutch design and lizards from four different populations to evaluate the robustness of temperature-responsive gene expression profiles. Embryos incubated at stressfully low incubation temperature expressed on average 20%... (More)

Many features of the development of reptiles are affected by temperature, but very little is known about how incubation temperature affects gene expression. Here, we provide a detailed case study of gene expression profiles in common wall lizard (Podarcis muralis) embryos developing at stressfully low (15°C) versus benign (24°C) temperature. For maximum comparability between the two temperature regimes, we selected a precise developmental stage early in embryogenesis defined by the number of somites. We used a split-clutch design and lizards from four different populations to evaluate the robustness of temperature-responsive gene expression profiles. Embryos incubated at stressfully low incubation temperature expressed on average 20% less total RNA than those incubated at benign temperatures, presumably reflecting lower rates of transcription at cool temperature. After normalizing for differences in total amounts of input RNA, we find that approximately 50% of all transcripts show significant expression differences between the two incubation temperatures. Transcripts with the most extreme changes in expression profiles are associated with transcriptional and translational regulation and chromatin remodeling, suggesting possible epigenetic mechanisms underlying acclimation of early embryos to cool temperature. We discuss our findings in light of current advances in the use of transcriptomic data to study how individuals acclimatize and populations adapt to thermal stress.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Embryo, epigenetics, lizards, thermal plasticity, transcriptome
in
Journal of experimental zoology. Part A, Ecological and integrative physiology
volume
329
issue
6-7
pages
351 - 361
publisher
John Wiley and Sons Inc.
external identifiers
  • scopus:85053422063
ISSN
2471-5638
DOI
10.1002/jez.2175
language
English
LU publication?
yes
id
37b38fc7-9036-4bc5-a8dd-52212cc1ae95
date added to LUP
2018-05-29 15:29:20
date last changed
2019-01-14 15:34:45
@article{37b38fc7-9036-4bc5-a8dd-52212cc1ae95,
  abstract     = {<p>Many features of the development of reptiles are affected by temperature, but very little is known about how incubation temperature affects gene expression. Here, we provide a detailed case study of gene expression profiles in common wall lizard (Podarcis muralis) embryos developing at stressfully low (15°C) versus benign (24°C) temperature. For maximum comparability between the two temperature regimes, we selected a precise developmental stage early in embryogenesis defined by the number of somites. We used a split-clutch design and lizards from four different populations to evaluate the robustness of temperature-responsive gene expression profiles. Embryos incubated at stressfully low incubation temperature expressed on average 20% less total RNA than those incubated at benign temperatures, presumably reflecting lower rates of transcription at cool temperature. After normalizing for differences in total amounts of input RNA, we find that approximately 50% of all transcripts show significant expression differences between the two incubation temperatures. Transcripts with the most extreme changes in expression profiles are associated with transcriptional and translational regulation and chromatin remodeling, suggesting possible epigenetic mechanisms underlying acclimation of early embryos to cool temperature. We discuss our findings in light of current advances in the use of transcriptomic data to study how individuals acclimatize and populations adapt to thermal stress.</p>},
  author       = {Feiner, Nathalie and Rago, Alfredo and While, Geoffrey M and Uller, Tobias},
  issn         = {2471-5638},
  keyword      = {Embryo,epigenetics,lizards,thermal plasticity,transcriptome},
  language     = {eng},
  month        = {05},
  number       = {6-7},
  pages        = {351--361},
  publisher    = {John Wiley and Sons Inc.},
  series       = {Journal of experimental zoology. Part A, Ecological and integrative physiology},
  title        = {Developmental plasticity in reptiles: Insights from temperature-dependent gene expression in wall lizard embryos : Insights from temperature-dependent gene expression in wall lizard embryos},
  url          = {http://dx.doi.org/10.1002/jez.2175},
  volume       = {329},
  year         = {2018},
}