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Wingless signaling in a large insect, the blowfly Lucilia sericata: A beautiful example of evolutionary developmental biology

Mellenthin, Katja LU ; Fahmy, Khalid LU ; Ali, Reda LU ; Hunding, A; Da Rocha Baez, Sol LU and Baumgartner, Stefan LU (2006) In Developmental Dynamics 235(2). p.347-360
Abstract
Blowflies are the primary facultative agent in causing myiasis of domestic sheep in the whole world and, at the same time, it is an important tool for forensic medicine. Surprisingly, and in contrast to its importance, almost no data regarding the embryology and molecular markers are known for this insect. In this report, we present a detailed description of the blowfly Lucilia sericata embryogenesis and of imaginal disc development. The embryogenesis of Lucilia strongly resembles that of Drosophila, despite their apparent size difference. Moreover, imaginal disc development appears to be equally well conserved. Through cloning, expression, and functional studies, we show that the Lucilia Wingless (Wg) protein is highly conserved between... (More)
Blowflies are the primary facultative agent in causing myiasis of domestic sheep in the whole world and, at the same time, it is an important tool for forensic medicine. Surprisingly, and in contrast to its importance, almost no data regarding the embryology and molecular markers are known for this insect. In this report, we present a detailed description of the blowfly Lucilia sericata embryogenesis and of imaginal disc development. The embryogenesis of Lucilia strongly resembles that of Drosophila, despite their apparent size difference. Moreover, imaginal disc development appears to be equally well conserved. Through cloning, expression, and functional studies, we show that the Lucilia Wingless (Wg) protein is highly conserved between the two species. We further show that parasegments are established in Lucilia, however, engrailed expression shows a more dynamic expression pattern than expected in comparison to Drosophila. Over-expression of Lucilia Wingless in Drosophila shows wingless-like wing phenotypes, suggesting that Lucilia Wingless blocks the signalling activity of Drosophila Wingless. Upon injection of wg dsRNA, we observe a "lawn of denticle" phenotype, closely resembling that of Drosophila. Due to the large size of the insect, the distance over which Wingless exerts signalling activity is up to three times larger than in Drosophila, yet the consequences are very similar. Our data demonstrate long-range wingless signaling mechanisms adapted for patterning large domains of naked cuticle and suggest signaling properties of Lucilia Wingless that are distinct from those of Drosophila Wingless. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
wingless, long-range patterning, Lucilia sericata, parasegments
in
Developmental Dynamics
volume
235
issue
2
pages
347 - 360
publisher
John Wiley & Sons
external identifiers
  • pmid:16258966
  • wos:000234672400006
  • scopus:30744437176
ISSN
1097-0177
DOI
10.1002/dvdy.20632
language
English
LU publication?
yes
id
37ceba4d-8128-44bb-889d-8afae8713331 (old id 419901)
date added to LUP
2007-08-17 10:33:39
date last changed
2019-10-06 03:24:25
@article{37ceba4d-8128-44bb-889d-8afae8713331,
  abstract     = {Blowflies are the primary facultative agent in causing myiasis of domestic sheep in the whole world and, at the same time, it is an important tool for forensic medicine. Surprisingly, and in contrast to its importance, almost no data regarding the embryology and molecular markers are known for this insect. In this report, we present a detailed description of the blowfly Lucilia sericata embryogenesis and of imaginal disc development. The embryogenesis of Lucilia strongly resembles that of Drosophila, despite their apparent size difference. Moreover, imaginal disc development appears to be equally well conserved. Through cloning, expression, and functional studies, we show that the Lucilia Wingless (Wg) protein is highly conserved between the two species. We further show that parasegments are established in Lucilia, however, engrailed expression shows a more dynamic expression pattern than expected in comparison to Drosophila. Over-expression of Lucilia Wingless in Drosophila shows wingless-like wing phenotypes, suggesting that Lucilia Wingless blocks the signalling activity of Drosophila Wingless. Upon injection of wg dsRNA, we observe a "lawn of denticle" phenotype, closely resembling that of Drosophila. Due to the large size of the insect, the distance over which Wingless exerts signalling activity is up to three times larger than in Drosophila, yet the consequences are very similar. Our data demonstrate long-range wingless signaling mechanisms adapted for patterning large domains of naked cuticle and suggest signaling properties of Lucilia Wingless that are distinct from those of Drosophila Wingless.},
  author       = {Mellenthin, Katja and Fahmy, Khalid and Ali, Reda and Hunding, A and Da Rocha Baez, Sol and Baumgartner, Stefan},
  issn         = {1097-0177},
  keyword      = {wingless,long-range patterning,Lucilia sericata,parasegments},
  language     = {eng},
  number       = {2},
  pages        = {347--360},
  publisher    = {John Wiley & Sons},
  series       = {Developmental Dynamics},
  title        = {Wingless signaling in a large insect, the blowfly Lucilia sericata: A beautiful example of evolutionary developmental biology},
  url          = {http://dx.doi.org/10.1002/dvdy.20632},
  volume       = {235},
  year         = {2006},
}