Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Optimization study on periodic counter-current chromatography integrated in a monoclonal antibody downstream process

Gomis-Fons, Joaquín LU ; Andersson, Niklas LU orcid and Nilsson, Bernt LU (2020) In Journal of Chromatography A 1621.
Abstract

An optimization study of an integrated periodic counter-current chromatography (PCC) process in a monoclonal antibody (mAb) downstream process at lab scale, is presented in this paper. The optimization was based on a mechanistic model of the breakthrough curve in the protein-A capture step. Productivity and resin utilization were the objective functions, while yield during the loading of the capture column was set as a constraint. Different integration approaches were considered, and the effect of the feed concentration, yield and the protein-A resin was studied. The breakthrough curve and the length of the product recovery, which depended on the integration approach, determined the process scheduling. Several optimal Pareto solutions... (More)

An optimization study of an integrated periodic counter-current chromatography (PCC) process in a monoclonal antibody (mAb) downstream process at lab scale, is presented in this paper. The optimization was based on a mechanistic model of the breakthrough curve in the protein-A capture step. Productivity and resin utilization were the objective functions, while yield during the loading of the capture column was set as a constraint. Different integration approaches were considered, and the effect of the feed concentration, yield and the protein-A resin was studied. The breakthrough curve and the length of the product recovery, which depended on the integration approach, determined the process scheduling. Several optimal Pareto solutions were obtained. At 0.5 mg mL−1 and 99% yield, a maximum productivity of 0.38 mg mL−1 min−1 with a resin utilization of 60% was obtained. On the other hand, the maximum resin utilization was 95% with a productivity of 0.10 mg mL−1 min−1. Due to the constraint of the process scheduling, a lower productivity could be achieved in the integration approaches with higher recovery time, which was more remarkable at higher concentrations. Therefore, it was shown that a holistic approach, where all the purification steps are considered in the process optimization, is needed to design a PCC in a downstream process.

(Less)
Please use this url to cite or link to this publication:
author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Downstream processing, Monoclonal antibody purification, Periodic counter-current chromatography, Process integration, Process optimization
in
Journal of Chromatography A
volume
1621
article number
461055
publisher
Elsevier
external identifiers
  • pmid:32276855
  • scopus:85082805028
ISSN
0021-9673
DOI
10.1016/j.chroma.2020.461055
language
English
LU publication?
yes
id
37eb9985-8bb5-45eb-ad19-65fc67acf218
date added to LUP
2020-04-24 16:54:41
date last changed
2024-05-02 07:38:06
@article{37eb9985-8bb5-45eb-ad19-65fc67acf218,
  abstract     = {{<p>An optimization study of an integrated periodic counter-current chromatography (PCC) process in a monoclonal antibody (mAb) downstream process at lab scale, is presented in this paper. The optimization was based on a mechanistic model of the breakthrough curve in the protein-A capture step. Productivity and resin utilization were the objective functions, while yield during the loading of the capture column was set as a constraint. Different integration approaches were considered, and the effect of the feed concentration, yield and the protein-A resin was studied. The breakthrough curve and the length of the product recovery, which depended on the integration approach, determined the process scheduling. Several optimal Pareto solutions were obtained. At 0.5 mg mL<sup>−1</sup> and 99% yield, a maximum productivity of 0.38 mg mL<sup>−1</sup> min<sup>−1</sup> with a resin utilization of 60% was obtained. On the other hand, the maximum resin utilization was 95% with a productivity of 0.10 mg mL<sup>−1</sup> min<sup>−1</sup>. Due to the constraint of the process scheduling, a lower productivity could be achieved in the integration approaches with higher recovery time, which was more remarkable at higher concentrations. Therefore, it was shown that a holistic approach, where all the purification steps are considered in the process optimization, is needed to design a PCC in a downstream process.</p>}},
  author       = {{Gomis-Fons, Joaquín and Andersson, Niklas and Nilsson, Bernt}},
  issn         = {{0021-9673}},
  keywords     = {{Downstream processing; Monoclonal antibody purification; Periodic counter-current chromatography; Process integration; Process optimization}},
  language     = {{eng}},
  month        = {{06}},
  publisher    = {{Elsevier}},
  series       = {{Journal of Chromatography A}},
  title        = {{Optimization study on periodic counter-current chromatography integrated in a monoclonal antibody downstream process}},
  url          = {{http://dx.doi.org/10.1016/j.chroma.2020.461055}},
  doi          = {{10.1016/j.chroma.2020.461055}},
  volume       = {{1621}},
  year         = {{2020}},
}