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Microfluidic impedance cytometer for platelet analysis.

Evander, Mikael LU ; Ricco, Antonio J; Morser, John; Kovacs, Gregory T A; Leung, Lawrence L K and Giovangrandi, Laurent (2013) In Lab on a Chip 13(4). p.722-729
Abstract
We present the design and performance characteristics of a platelet analysis platform based on a microfluidic impedance cytometer. Dielectrophoretic focusing is used to centre cells in a fluid stream, which then forms the core of a two-phase flow (dielectric focusing). This flow then passes between electrodes for analysis by differential impedance spectroscopy at multiple frequencies from 280 kHz to 4 MHz. This approach increases the signal-to-noise ratio relative to a single-phase, unfocused stream, while minimising the shear forces to which the cells are subjected. The percentage of activated platelets before and after passage through the chip was measured using flow cytometry, and no significant change was measured. Measuring the... (More)
We present the design and performance characteristics of a platelet analysis platform based on a microfluidic impedance cytometer. Dielectrophoretic focusing is used to centre cells in a fluid stream, which then forms the core of a two-phase flow (dielectric focusing). This flow then passes between electrodes for analysis by differential impedance spectroscopy at multiple frequencies from 280 kHz to 4 MHz. This approach increases the signal-to-noise ratio relative to a single-phase, unfocused stream, while minimising the shear forces to which the cells are subjected. The percentage of activated platelets before and after passage through the chip was measured using flow cytometry, and no significant change was measured. Measuring the in-phase amplitude at a single frequency is sufficient to distinguish platelets from erythrocytes. Using multi-frequency impedance measurements and discriminant analysis, resting platelets can be discriminated from activated platelets. This multifrequency impedance cytometer therefore allows ready determination of the degree of platelet activation in blood samples. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Lab on a Chip
volume
13
issue
4
pages
722 - 729
publisher
Royal Society of Chemistry
external identifiers
  • pmid:23282651
  • wos:000313971300028
  • scopus:84875779608
ISSN
1473-0189
DOI
10.1039/c2lc40896a
language
English
LU publication?
yes
id
381c5801-bdfb-4c81-b7d5-395ade08d5c8 (old id 3439051)
date added to LUP
2013-02-05 12:40:42
date last changed
2018-10-03 10:50:31
@article{381c5801-bdfb-4c81-b7d5-395ade08d5c8,
  abstract     = {We present the design and performance characteristics of a platelet analysis platform based on a microfluidic impedance cytometer. Dielectrophoretic focusing is used to centre cells in a fluid stream, which then forms the core of a two-phase flow (dielectric focusing). This flow then passes between electrodes for analysis by differential impedance spectroscopy at multiple frequencies from 280 kHz to 4 MHz. This approach increases the signal-to-noise ratio relative to a single-phase, unfocused stream, while minimising the shear forces to which the cells are subjected. The percentage of activated platelets before and after passage through the chip was measured using flow cytometry, and no significant change was measured. Measuring the in-phase amplitude at a single frequency is sufficient to distinguish platelets from erythrocytes. Using multi-frequency impedance measurements and discriminant analysis, resting platelets can be discriminated from activated platelets. This multifrequency impedance cytometer therefore allows ready determination of the degree of platelet activation in blood samples.},
  author       = {Evander, Mikael and Ricco, Antonio J and Morser, John and Kovacs, Gregory T A and Leung, Lawrence L K and Giovangrandi, Laurent},
  issn         = {1473-0189},
  language     = {eng},
  number       = {4},
  pages        = {722--729},
  publisher    = {Royal Society of Chemistry},
  series       = {Lab on a Chip},
  title        = {Microfluidic impedance cytometer for platelet analysis.},
  url          = {http://dx.doi.org/10.1039/c2lc40896a},
  volume       = {13},
  year         = {2013},
}