High expression of 5-lipoxygenase in normal and malignant mantle zone B lymphocytes
(2009) In BMC Immunology 10.- Abstract
BACKGROUND: Human B lymphocytes can produce leukotriene B4 but the biological function of the 5-lipoxygenase (5-LO) pathway in B cells is unclear. In order to better understand and define the role of 5-LO in B cells, we investigated the expression of 5-LO mRNA and protein in subsets of B cells from human tonsils and different types of B cell lymphoma.
RESULTS: Based on RT-PCR and western blot/immunohistochemical staining, with a polyclonal antibody raised against 5-LO, high expression of 5-LO was found in mantle zone B cells from tonsils. By contrast, only a weak expression of 5-LO was detected in germinal centre cells and no expression in plasma cells from tonsils. This pattern of 5-LO expression was preserved in malignant... (More)
BACKGROUND: Human B lymphocytes can produce leukotriene B4 but the biological function of the 5-lipoxygenase (5-LO) pathway in B cells is unclear. In order to better understand and define the role of 5-LO in B cells, we investigated the expression of 5-LO mRNA and protein in subsets of B cells from human tonsils and different types of B cell lymphoma.
RESULTS: Based on RT-PCR and western blot/immunohistochemical staining, with a polyclonal antibody raised against 5-LO, high expression of 5-LO was found in mantle zone B cells from tonsils. By contrast, only a weak expression of 5-LO was detected in germinal centre cells and no expression in plasma cells from tonsils. This pattern of 5-LO expression was preserved in malignant lymphoma with high expression in mantle B cell lymphoma (MCL) and weak or no expression in follicular lymphoma. Primary leukemized MCL, so called B-prolymphocytic leukaemia cells, and MCL cell lines also expressed 5-LO and readily produced LTB4 after activation.
CONCLUSION: The present report demonstrates the expression of 5-LO mainly in normal and malignant mantle zone B cells while the expression is low or absent in germinal centre B cells and plasma cells, indicating a role of the 5-LO pathway in B cells before the cells finally differentiate to plasma cells.
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- author
- Mahshid, Yilmaz ; Lisy, Marcus-René ; Wang, Xiao LU ; Spanbroek, Rainer ; Flygare, Jenny ; Christensson, Birger ; Björkholm, Magnus ; Sander, Birgitta ; Habenicht, Andreas J R and Claesson, Hans-Erik
- publishing date
- 2009-01-09
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Arachidonate 5-Lipoxygenase/biosynthesis, B-Lymphocyte Subsets/enzymology, B-Lymphocytes/enzymology, Blotting, Western, Cell Differentiation, Cell Line, Cell Transformation, Neoplastic, Gene Expression Regulation, Enzymologic, Gene Expression Regulation, Leukemic, Humans, Immunity, Cellular, Immunologic Memory, Immunophenotyping, Leukemia, Prolymphocytic, B-Cell/enzymology, Leukotriene B4/metabolism, Lymphocyte Activation, Lymphoma, Follicular/enzymology, Lymphoma, Mantle-Cell/enzymology, Microscopy, Fluorescence, Palatine Tonsil/cytology, Polymerase Chain Reaction, Signal Transduction
- in
- BMC Immunology
- volume
- 10
- article number
- 2
- publisher
- BioMed Central (BMC)
- external identifiers
-
- pmid:19134178
- scopus:59349106832
- ISSN
- 1471-2172
- DOI
- 10.1186/1471-2172-10-2
- language
- English
- LU publication?
- no
- id
- 38252827-505a-4384-8053-3de46d7fc9c3
- date added to LUP
- 2021-05-07 09:22:33
- date last changed
- 2024-05-04 08:01:18
@article{38252827-505a-4384-8053-3de46d7fc9c3,
abstract = {{<p>BACKGROUND: Human B lymphocytes can produce leukotriene B4 but the biological function of the 5-lipoxygenase (5-LO) pathway in B cells is unclear. In order to better understand and define the role of 5-LO in B cells, we investigated the expression of 5-LO mRNA and protein in subsets of B cells from human tonsils and different types of B cell lymphoma.</p><p>RESULTS: Based on RT-PCR and western blot/immunohistochemical staining, with a polyclonal antibody raised against 5-LO, high expression of 5-LO was found in mantle zone B cells from tonsils. By contrast, only a weak expression of 5-LO was detected in germinal centre cells and no expression in plasma cells from tonsils. This pattern of 5-LO expression was preserved in malignant lymphoma with high expression in mantle B cell lymphoma (MCL) and weak or no expression in follicular lymphoma. Primary leukemized MCL, so called B-prolymphocytic leukaemia cells, and MCL cell lines also expressed 5-LO and readily produced LTB4 after activation.</p><p>CONCLUSION: The present report demonstrates the expression of 5-LO mainly in normal and malignant mantle zone B cells while the expression is low or absent in germinal centre B cells and plasma cells, indicating a role of the 5-LO pathway in B cells before the cells finally differentiate to plasma cells.</p>}},
author = {{Mahshid, Yilmaz and Lisy, Marcus-René and Wang, Xiao and Spanbroek, Rainer and Flygare, Jenny and Christensson, Birger and Björkholm, Magnus and Sander, Birgitta and Habenicht, Andreas J R and Claesson, Hans-Erik}},
issn = {{1471-2172}},
keywords = {{Arachidonate 5-Lipoxygenase/biosynthesis; B-Lymphocyte Subsets/enzymology; B-Lymphocytes/enzymology; Blotting, Western; Cell Differentiation; Cell Line; Cell Transformation, Neoplastic; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Leukemic; Humans; Immunity, Cellular; Immunologic Memory; Immunophenotyping; Leukemia, Prolymphocytic, B-Cell/enzymology; Leukotriene B4/metabolism; Lymphocyte Activation; Lymphoma, Follicular/enzymology; Lymphoma, Mantle-Cell/enzymology; Microscopy, Fluorescence; Palatine Tonsil/cytology; Polymerase Chain Reaction; Signal Transduction}},
language = {{eng}},
month = {{01}},
publisher = {{BioMed Central (BMC)}},
series = {{BMC Immunology}},
title = {{High expression of 5-lipoxygenase in normal and malignant mantle zone B lymphocytes}},
url = {{http://dx.doi.org/10.1186/1471-2172-10-2}},
doi = {{10.1186/1471-2172-10-2}},
volume = {{10}},
year = {{2009}},
}