Androgen-sensitive human prostate cancer cells, LNCaP, produce both N-terminally mature and truncated prostate-specific antigen isoforms
(1998) In European Journal of Biochemistry 255(2). p.329-335- Abstract
- To characterize prostate-specific antigen (PSA) produced by cancer cells, different isoforms of PSA secreted by the human prostate cancer cells, LNCaP, were purified. LNCaP-PSA production was induced by synthetic androgen, R1881. LNCaP-PSA was separated into four pools. The molecular mass of LNCaP-PSA isoforms in these pools was 34 kDa under reducing conditions and 29 kDa under nonreducing conditions on SDS/PAGE. pI of LNCaP-PSA isoforms varied from 6.8 to 8.2. Pool A had the highest specific activity, 37 nmol/(minxmg). All the pools formed stable complexes with alpha 1-antichymotrypsin and alpha 2-macroglobulin. The Fools contained 10-60% of N-terminally correctly processed LNCaP-PSA isoforms. According to the molecular modelling, the... (More)
- To characterize prostate-specific antigen (PSA) produced by cancer cells, different isoforms of PSA secreted by the human prostate cancer cells, LNCaP, were purified. LNCaP-PSA production was induced by synthetic androgen, R1881. LNCaP-PSA was separated into four pools. The molecular mass of LNCaP-PSA isoforms in these pools was 34 kDa under reducing conditions and 29 kDa under nonreducing conditions on SDS/PAGE. pI of LNCaP-PSA isoforms varied from 6.8 to 8.2. Pool A had the highest specific activity, 37 nmol/(minxmg). All the pools formed stable complexes with alpha 1-antichymotrypsin and alpha 2-macroglobulin. The Fools contained 10-60% of N-terminally correctly processed LNCaP-PSA isoforms. According to the molecular modelling, the addition or deletion of two or four N-terminal amino acids could affect the three-dimensional structure and thereby remarkably reduce the enzyme activity of LNCaP-PSA. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3852516
- author
- Herrala, A ; Kurkela, R ; Vihinen, Mauno LU ; Kalkkinen, N and Vihko, P
- publishing date
- 1998
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- prostate cancer, N-terminal sequencing, molecular modeling, standardization, enzyme activity
- in
- European Journal of Biochemistry
- volume
- 255
- issue
- 2
- pages
- 329 - 335
- publisher
- Wiley-Blackwell
- external identifiers
-
- wos:000074954500001
- scopus:0032528007
- ISSN
- 0014-2956
- DOI
- 10.1046/j.1432-1327.1998.2550329.x
- language
- English
- LU publication?
- no
- id
- 74dfd693-3417-4be4-8330-2d533e3741d2 (old id 3852516)
- date added to LUP
- 2016-04-01 16:56:55
- date last changed
- 2022-01-28 23:17:28
@article{74dfd693-3417-4be4-8330-2d533e3741d2, abstract = {{To characterize prostate-specific antigen (PSA) produced by cancer cells, different isoforms of PSA secreted by the human prostate cancer cells, LNCaP, were purified. LNCaP-PSA production was induced by synthetic androgen, R1881. LNCaP-PSA was separated into four pools. The molecular mass of LNCaP-PSA isoforms in these pools was 34 kDa under reducing conditions and 29 kDa under nonreducing conditions on SDS/PAGE. pI of LNCaP-PSA isoforms varied from 6.8 to 8.2. Pool A had the highest specific activity, 37 nmol/(minxmg). All the pools formed stable complexes with alpha 1-antichymotrypsin and alpha 2-macroglobulin. The Fools contained 10-60% of N-terminally correctly processed LNCaP-PSA isoforms. According to the molecular modelling, the addition or deletion of two or four N-terminal amino acids could affect the three-dimensional structure and thereby remarkably reduce the enzyme activity of LNCaP-PSA.}}, author = {{Herrala, A and Kurkela, R and Vihinen, Mauno and Kalkkinen, N and Vihko, P}}, issn = {{0014-2956}}, keywords = {{prostate cancer; N-terminal sequencing; molecular modeling; standardization; enzyme activity}}, language = {{eng}}, number = {{2}}, pages = {{329--335}}, publisher = {{Wiley-Blackwell}}, series = {{European Journal of Biochemistry}}, title = {{Androgen-sensitive human prostate cancer cells, LNCaP, produce both N-terminally mature and truncated prostate-specific antigen isoforms}}, url = {{http://dx.doi.org/10.1046/j.1432-1327.1998.2550329.x}}, doi = {{10.1046/j.1432-1327.1998.2550329.x}}, volume = {{255}}, year = {{1998}}, }