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Androgen-sensitive human prostate cancer cells, LNCaP, produce both N-terminally mature and truncated prostate-specific antigen isoforms

Herrala, A; Kurkela, R; Vihinen, Mauno LU ; Kalkkinen, N and Vihko, P (1998) In European Journal of Biochemistry 255(2). p.329-335
Abstract
To characterize prostate-specific antigen (PSA) produced by cancer cells, different isoforms of PSA secreted by the human prostate cancer cells, LNCaP, were purified. LNCaP-PSA production was induced by synthetic androgen, R1881. LNCaP-PSA was separated into four pools. The molecular mass of LNCaP-PSA isoforms in these pools was 34 kDa under reducing conditions and 29 kDa under nonreducing conditions on SDS/PAGE. pI of LNCaP-PSA isoforms varied from 6.8 to 8.2. Pool A had the highest specific activity, 37 nmol/(minxmg). All the pools formed stable complexes with alpha 1-antichymotrypsin and alpha 2-macroglobulin. The Fools contained 10-60% of N-terminally correctly processed LNCaP-PSA isoforms. According to the molecular modelling, the... (More)
To characterize prostate-specific antigen (PSA) produced by cancer cells, different isoforms of PSA secreted by the human prostate cancer cells, LNCaP, were purified. LNCaP-PSA production was induced by synthetic androgen, R1881. LNCaP-PSA was separated into four pools. The molecular mass of LNCaP-PSA isoforms in these pools was 34 kDa under reducing conditions and 29 kDa under nonreducing conditions on SDS/PAGE. pI of LNCaP-PSA isoforms varied from 6.8 to 8.2. Pool A had the highest specific activity, 37 nmol/(minxmg). All the pools formed stable complexes with alpha 1-antichymotrypsin and alpha 2-macroglobulin. The Fools contained 10-60% of N-terminally correctly processed LNCaP-PSA isoforms. According to the molecular modelling, the addition or deletion of two or four N-terminal amino acids could affect the three-dimensional structure and thereby remarkably reduce the enzyme activity of LNCaP-PSA. (Less)
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author
publishing date
type
Contribution to journal
publication status
published
subject
keywords
prostate cancer, N-terminal sequencing, molecular modeling, standardization, enzyme activity
in
European Journal of Biochemistry
volume
255
issue
2
pages
329 - 335
publisher
Wiley-Blackwell
external identifiers
  • wos:000074954500001
  • scopus:0032528007
ISSN
0014-2956
DOI
10.1046/j.1432-1327.1998.2550329.x
language
English
LU publication?
no
id
74dfd693-3417-4be4-8330-2d533e3741d2 (old id 3852516)
date added to LUP
2013-06-28 14:28:25
date last changed
2017-01-01 07:19:35
@article{74dfd693-3417-4be4-8330-2d533e3741d2,
  abstract     = {To characterize prostate-specific antigen (PSA) produced by cancer cells, different isoforms of PSA secreted by the human prostate cancer cells, LNCaP, were purified. LNCaP-PSA production was induced by synthetic androgen, R1881. LNCaP-PSA was separated into four pools. The molecular mass of LNCaP-PSA isoforms in these pools was 34 kDa under reducing conditions and 29 kDa under nonreducing conditions on SDS/PAGE. pI of LNCaP-PSA isoforms varied from 6.8 to 8.2. Pool A had the highest specific activity, 37 nmol/(minxmg). All the pools formed stable complexes with alpha 1-antichymotrypsin and alpha 2-macroglobulin. The Fools contained 10-60% of N-terminally correctly processed LNCaP-PSA isoforms. According to the molecular modelling, the addition or deletion of two or four N-terminal amino acids could affect the three-dimensional structure and thereby remarkably reduce the enzyme activity of LNCaP-PSA.},
  author       = {Herrala, A and Kurkela, R and Vihinen, Mauno and Kalkkinen, N and Vihko, P},
  issn         = {0014-2956},
  keyword      = {prostate cancer,N-terminal sequencing,molecular modeling,standardization,enzyme activity},
  language     = {eng},
  number       = {2},
  pages        = {329--335},
  publisher    = {Wiley-Blackwell},
  series       = {European Journal of Biochemistry},
  title        = {Androgen-sensitive human prostate cancer cells, LNCaP, produce both N-terminally mature and truncated prostate-specific antigen isoforms},
  url          = {http://dx.doi.org/10.1046/j.1432-1327.1998.2550329.x},
  volume       = {255},
  year         = {1998},
}