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Solution structure of the SH3 domain from Bruton's tyrosine kinase

Hansson, H; Mattsson, PT; Allard, P; Haapaniemi, P; Vihinen, Mauno LU ; Smith, CIE and Hard, T (1998) In Biochemistry 37(9). p.2912-2924
Abstract
X-linked agammaglobulinemia (XLA) is a heritable immunodeficiency caused by mutations in the gene coding for Bruton's tyrosine kinase (Btk). Btk belongs to the Tec family of tyrosine kinases. Each member of the family contains five regions and mutations causing XLA have been isolated in all five regions. We have determined the solution structure of the Src homology 3 (SH3) domain of Btk using two-and three-dimensional nuclear magnetic resonance (NMR) spectroscopy on natural abundance and N-15-labeled protein material. The structure determination is complemented by investigation of backbone dynamics based on N-15 NMR relaxation. The Btk SH3 forms a well-defined structure and shows the typical SH3 topology of two shea antiparallel... (More)
X-linked agammaglobulinemia (XLA) is a heritable immunodeficiency caused by mutations in the gene coding for Bruton's tyrosine kinase (Btk). Btk belongs to the Tec family of tyrosine kinases. Each member of the family contains five regions and mutations causing XLA have been isolated in all five regions. We have determined the solution structure of the Src homology 3 (SH3) domain of Btk using two-and three-dimensional nuclear magnetic resonance (NMR) spectroscopy on natural abundance and N-15-labeled protein material. The structure determination is complemented by investigation of backbone dynamics based on N-15 NMR relaxation. The Btk SH3 forms a well-defined structure and shows the typical SH3 topology of two shea antiparallel beta-sheets packed almost perpendicular to each other in a sandwich-like fold. The N- and C-termini are more flexible as are peptide fragments in the RT and n-Src loops. The studied Btk SH3 fragment adopts two slowly interconverting conformations with a relative concentration ratio of 7:1. The overall fold of the minor form is similar to that of the major form, as judged on the basis of observed NOE connectivities and small chemical shift differences. A tryptophan (W251) ring flip is the favored mechanism for interconversion, although other possibilities cannot be excluded. The side chain of Y223, which becomes autophosphorylated upon activation of Btk, is exposed within the potential SH3 ligand binding site. Finally, we compare the present Btk SH3 structure with other SH3 structures. (Less)
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author
publishing date
type
Contribution to journal
publication status
published
subject
in
Biochemistry
volume
37
issue
9
pages
2912 - 2924
publisher
The American Chemical Society
external identifiers
  • wos:000072523600023
  • scopus:0032478131
ISSN
0006-2960
DOI
10.1021/bi972409f
language
English
LU publication?
no
id
bfc83250-3189-4eab-8a4c-e801a5883b31 (old id 3852562)
date added to LUP
2013-06-28 14:29:10
date last changed
2017-04-16 03:27:47
@article{bfc83250-3189-4eab-8a4c-e801a5883b31,
  abstract     = {X-linked agammaglobulinemia (XLA) is a heritable immunodeficiency caused by mutations in the gene coding for Bruton's tyrosine kinase (Btk). Btk belongs to the Tec family of tyrosine kinases. Each member of the family contains five regions and mutations causing XLA have been isolated in all five regions. We have determined the solution structure of the Src homology 3 (SH3) domain of Btk using two-and three-dimensional nuclear magnetic resonance (NMR) spectroscopy on natural abundance and N-15-labeled protein material. The structure determination is complemented by investigation of backbone dynamics based on N-15 NMR relaxation. The Btk SH3 forms a well-defined structure and shows the typical SH3 topology of two shea antiparallel beta-sheets packed almost perpendicular to each other in a sandwich-like fold. The N- and C-termini are more flexible as are peptide fragments in the RT and n-Src loops. The studied Btk SH3 fragment adopts two slowly interconverting conformations with a relative concentration ratio of 7:1. The overall fold of the minor form is similar to that of the major form, as judged on the basis of observed NOE connectivities and small chemical shift differences. A tryptophan (W251) ring flip is the favored mechanism for interconversion, although other possibilities cannot be excluded. The side chain of Y223, which becomes autophosphorylated upon activation of Btk, is exposed within the potential SH3 ligand binding site. Finally, we compare the present Btk SH3 structure with other SH3 structures.},
  author       = {Hansson, H and Mattsson, PT and Allard, P and Haapaniemi, P and Vihinen, Mauno and Smith, CIE and Hard, T},
  issn         = {0006-2960},
  language     = {eng},
  number       = {9},
  pages        = {2912--2924},
  publisher    = {The American Chemical Society},
  series       = {Biochemistry},
  title        = {Solution structure of the SH3 domain from Bruton's tyrosine kinase},
  url          = {http://dx.doi.org/10.1021/bi972409f},
  volume       = {37},
  year         = {1998},
}