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MODELING OF PROSTATE-SPECIFIC ANTIGEN AND HUMAN GLANDULAR KALLIKREIN STRUCTURES

Vihinen, Mauno LU (1994) In Biochemical and Biophysical Research Communications 204(3). p.1251-1256
Abstract
The three dimensional structures of human prostate specific antigen (PSA) and glandular kallikrein (hGK) were modeled based on porcine pancreatic kallikrein A. High sequence similarity and conserved framework of serine proteases enabled accurate modeling. The catalytic site region consisting of catalytic triad, residues forming oxyanion hole and main-chain substrate binding residues were conserved. The substrate specificity pocket of PSA resembles that of chymotrypsin and hGK is most related with tonin. The models were used to predict interactions with substrate and inhibitor molecules. The models are valuable in interpreting mutant and epitope mapping data as well as when modifying properties of the proteases or when developing diagnostic... (More)
The three dimensional structures of human prostate specific antigen (PSA) and glandular kallikrein (hGK) were modeled based on porcine pancreatic kallikrein A. High sequence similarity and conserved framework of serine proteases enabled accurate modeling. The catalytic site region consisting of catalytic triad, residues forming oxyanion hole and main-chain substrate binding residues were conserved. The substrate specificity pocket of PSA resembles that of chymotrypsin and hGK is most related with tonin. The models were used to predict interactions with substrate and inhibitor molecules. The models are valuable in interpreting mutant and epitope mapping data as well as when modifying properties of the proteases or when developing diagnostic detection methods for prostatic cancer. (C) 1994 Academic Press, Inc. (Less)
Please use this url to cite or link to this publication:
author
publishing date
type
Contribution to journal
publication status
published
subject
in
Biochemical and Biophysical Research Communications
volume
204
issue
3
pages
1251 - 1256
publisher
Elsevier
external identifiers
  • wos:A1994PR98000036
  • scopus:0028116277
ISSN
1090-2104
DOI
10.1006/bbrc.1994.2597
language
English
LU publication?
no
id
2d56d058-2e00-4180-88bb-fad071f9070c (old id 3853343)
date added to LUP
2013-06-28 14:48:08
date last changed
2017-01-01 06:56:26
@article{2d56d058-2e00-4180-88bb-fad071f9070c,
  abstract     = {The three dimensional structures of human prostate specific antigen (PSA) and glandular kallikrein (hGK) were modeled based on porcine pancreatic kallikrein A. High sequence similarity and conserved framework of serine proteases enabled accurate modeling. The catalytic site region consisting of catalytic triad, residues forming oxyanion hole and main-chain substrate binding residues were conserved. The substrate specificity pocket of PSA resembles that of chymotrypsin and hGK is most related with tonin. The models were used to predict interactions with substrate and inhibitor molecules. The models are valuable in interpreting mutant and epitope mapping data as well as when modifying properties of the proteases or when developing diagnostic detection methods for prostatic cancer. (C) 1994 Academic Press, Inc.},
  author       = {Vihinen, Mauno},
  issn         = {1090-2104},
  language     = {eng},
  number       = {3},
  pages        = {1251--1256},
  publisher    = {Elsevier},
  series       = {Biochemical and Biophysical Research Communications},
  title        = {MODELING OF PROSTATE-SPECIFIC ANTIGEN AND HUMAN GLANDULAR KALLIKREIN STRUCTURES},
  url          = {http://dx.doi.org/10.1006/bbrc.1994.2597},
  volume       = {204},
  year         = {1994},
}