MODELING OF PROSTATE-SPECIFIC ANTIGEN AND HUMAN GLANDULAR KALLIKREIN STRUCTURES
Vihinen, Mauno LU
(1994)
In Biochemical and Biophysical Research Communications
204(3).
p.1251-1256
- Abstract
- The three dimensional structures of human prostate specific antigen (PSA) and glandular kallikrein (hGK) were modeled based on porcine pancreatic kallikrein A. High sequence similarity and conserved framework of serine proteases enabled accurate modeling. The catalytic site region consisting of catalytic triad, residues forming oxyanion hole and main-chain substrate binding residues were conserved. The substrate specificity pocket of PSA resembles that of chymotrypsin and hGK is most related with tonin. The models were used to predict interactions with substrate and inhibitor molecules. The models are valuable in interpreting mutant and epitope mapping data as well as when modifying properties of the proteases or when developing diagnostic... (More)
- The three dimensional structures of human prostate specific antigen (PSA) and glandular kallikrein (hGK) were modeled based on porcine pancreatic kallikrein A. High sequence similarity and conserved framework of serine proteases enabled accurate modeling. The catalytic site region consisting of catalytic triad, residues forming oxyanion hole and main-chain substrate binding residues were conserved. The substrate specificity pocket of PSA resembles that of chymotrypsin and hGK is most related with tonin. The models were used to predict interactions with substrate and inhibitor molecules. The models are valuable in interpreting mutant and epitope mapping data as well as when modifying properties of the proteases or when developing diagnostic detection methods for prostatic cancer. (C) 1994 Academic Press, Inc. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3853343
- author
- Vihinen, Mauno
LU
- publishing date
- 1994
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Biochemical and Biophysical Research Communications
- volume
- 204
- issue
- 3
- pages
- 1251 - 1256
- publisher
- Elsevier
- external identifiers
-
- wos:A1994PR98000036
- scopus:0028116277
- ISSN
- 1090-2104
- DOI
- 10.1006/bbrc.1994.2597
- language
- English
- LU publication?
- no
- id
- 2d56d058-2e00-4180-88bb-fad071f9070c (old id 3853343)
- date added to LUP
- 2016-04-01 16:07:40
- date last changed
- 2021-01-03 10:46:53
@article{2d56d058-2e00-4180-88bb-fad071f9070c,
abstract = {{The three dimensional structures of human prostate specific antigen (PSA) and glandular kallikrein (hGK) were modeled based on porcine pancreatic kallikrein A. High sequence similarity and conserved framework of serine proteases enabled accurate modeling. The catalytic site region consisting of catalytic triad, residues forming oxyanion hole and main-chain substrate binding residues were conserved. The substrate specificity pocket of PSA resembles that of chymotrypsin and hGK is most related with tonin. The models were used to predict interactions with substrate and inhibitor molecules. The models are valuable in interpreting mutant and epitope mapping data as well as when modifying properties of the proteases or when developing diagnostic detection methods for prostatic cancer. (C) 1994 Academic Press, Inc.}},
author = {{Vihinen, Mauno}},
issn = {{1090-2104}},
language = {{eng}},
number = {{3}},
pages = {{1251--1256}},
publisher = {{Elsevier}},
series = {{Biochemical and Biophysical Research Communications}},
title = {{MODELING OF PROSTATE-SPECIFIC ANTIGEN AND HUMAN GLANDULAR KALLIKREIN STRUCTURES}},
url = {{http://dx.doi.org/10.1006/bbrc.1994.2597}},
doi = {{10.1006/bbrc.1994.2597}},
volume = {{204}},
year = {{1994}},
}