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Urinary Apolipoprotein M Could Be Used as a Biomarker of Acute Renal Injury: An Ischemia-Reperfusion Injury Model of Kidney in Rat

Xu, X. -L. ; Mao, Q. -Y. ; Luo, G. -H. ; Nilsson-Ehle, Peter LU ; He, X. -Z. and Xu, Ning LU (2013) In Transplantation Proceedings 45(6). p.2476-2479
Abstract
Background. It has been well documented that apolipoprotein M (apoM) is principally expressed in hepatocytes as well as renal tubular epithelial cells. The importance of apoM in the kidney is unknown. In the present study we examined urinary any apoM after short-term ischemia-reperfusion injury (IRI) of kidney in a rat model. Methods. The kidneys of 11 male Sprague-Dawley rats were rendered ischemic for 45 minutes followed by different intervals of reperfusion. Serum and urine apoM concentrations were determined using a dot-blot analysis with specific rabbit anti-human apoM antibodies that cross-react with rat apoM. Serum concentrations of blood urea nitrogen (BUN) and creatinine (Cr) were determined using standard clinical automated... (More)
Background. It has been well documented that apolipoprotein M (apoM) is principally expressed in hepatocytes as well as renal tubular epithelial cells. The importance of apoM in the kidney is unknown. In the present study we examined urinary any apoM after short-term ischemia-reperfusion injury (IRI) of kidney in a rat model. Methods. The kidneys of 11 male Sprague-Dawley rats were rendered ischemic for 45 minutes followed by different intervals of reperfusion. Serum and urine apoM concentrations were determined using a dot-blot analysis with specific rabbit anti-human apoM antibodies that cross-react with rat apoM. Serum concentrations of blood urea nitrogen (BUN) and creatinine (Cr) were determined using standard clinical automated analyses. Results. BUN was significantly elevated after 45 minutes of ischemia followed by 24 hours of reperfusion; serum Cr concentrations were also significantly increased at 6 and 24 hours of reperfusion. Interestingly, similar to BUN and Cr, serum apoM concentrations were significantly increased after ischemia for 45 minutes alone and after 2 hours of reperfusion. Urinary apoM concentrations were obviously increased after 2 h as well as 6 hours of reperfusion. Conclusion. apoM showed characteristics of an acute-phase reactive protein; its occurrence in urine may be considered to be a biomarker of acute renal injury. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Transplantation Proceedings
volume
45
issue
6
pages
2476 - 2479
publisher
Elsevier
external identifiers
  • wos:000323852000071
  • scopus:84882259224
  • pmid:23953565
ISSN
0041-1345
DOI
10.1016/j.transproceed.2013.04.009
language
English
LU publication?
yes
id
3884e1ec-a8c5-47f4-a606-60a1c8245195 (old id 4062560)
date added to LUP
2016-04-01 10:20:37
date last changed
2020-01-05 05:54:38
@article{3884e1ec-a8c5-47f4-a606-60a1c8245195,
  abstract     = {Background. It has been well documented that apolipoprotein M (apoM) is principally expressed in hepatocytes as well as renal tubular epithelial cells. The importance of apoM in the kidney is unknown. In the present study we examined urinary any apoM after short-term ischemia-reperfusion injury (IRI) of kidney in a rat model. Methods. The kidneys of 11 male Sprague-Dawley rats were rendered ischemic for 45 minutes followed by different intervals of reperfusion. Serum and urine apoM concentrations were determined using a dot-blot analysis with specific rabbit anti-human apoM antibodies that cross-react with rat apoM. Serum concentrations of blood urea nitrogen (BUN) and creatinine (Cr) were determined using standard clinical automated analyses. Results. BUN was significantly elevated after 45 minutes of ischemia followed by 24 hours of reperfusion; serum Cr concentrations were also significantly increased at 6 and 24 hours of reperfusion. Interestingly, similar to BUN and Cr, serum apoM concentrations were significantly increased after ischemia for 45 minutes alone and after 2 hours of reperfusion. Urinary apoM concentrations were obviously increased after 2 h as well as 6 hours of reperfusion. Conclusion. apoM showed characteristics of an acute-phase reactive protein; its occurrence in urine may be considered to be a biomarker of acute renal injury.},
  author       = {Xu, X. -L. and Mao, Q. -Y. and Luo, G. -H. and Nilsson-Ehle, Peter and He, X. -Z. and Xu, Ning},
  issn         = {0041-1345},
  language     = {eng},
  number       = {6},
  pages        = {2476--2479},
  publisher    = {Elsevier},
  series       = {Transplantation Proceedings},
  title        = {Urinary Apolipoprotein M Could Be Used as a Biomarker of Acute Renal Injury: An Ischemia-Reperfusion Injury Model of Kidney in Rat},
  url          = {http://dx.doi.org/10.1016/j.transproceed.2013.04.009},
  doi          = {10.1016/j.transproceed.2013.04.009},
  volume       = {45},
  year         = {2013},
}