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A comparative study of capacitive immunosensors based on self-assembled monolayers formed from thiourea, thioctic acid, and 3-mercaptopropionic acid

Limbut, Warakorn ; Kanatharana, Proespichaya ; Mattiasson, Bo LU ; Asawatreratanakul, Punnee and Thavarungkul, Panote (2006) In Biosensors & Bioelectronics 22(2). p.233-240
Abstract
A procedure was developed for the covalent coupling of anti-alpha-fetoprotein antibody (anti-AFP) to a gold surface modified with a self-assembled monolayer (SAM) of thiourea (TU). The performance of the SAM-antibody layer was compared to those of similar layers based on thioctic acid (TA) and 3-mercaptopropionic acid (MPA) by using flow injection capacitive immunosensor system. Covalent coupling of anti-AFP on self-assembled thiourea monolayer (SATUM) modified gold electrode can be used to detect alpha-fetoprotein with high efficiency, similar sensitivity, the same linear range (0.01-10 mu g l(-1)) and detection limit (10ng l(-1)) as those obtained from sensors based on self-assembled thioctic acid monolayer (SATAM) and self-assembled... (More)
A procedure was developed for the covalent coupling of anti-alpha-fetoprotein antibody (anti-AFP) to a gold surface modified with a self-assembled monolayer (SAM) of thiourea (TU). The performance of the SAM-antibody layer was compared to those of similar layers based on thioctic acid (TA) and 3-mercaptopropionic acid (MPA) by using flow injection capacitive immunosensor system. Covalent coupling of anti-AFP on self-assembled thiourea monolayer (SATUM) modified gold electrode can be used to detect alpha-fetoprotein with high efficiency, similar sensitivity, the same linear range (0.01-10 mu g l(-1)) and detection limit (10ng l(-1)) as those obtained from sensors based on self-assembled thioctic acid monolayer (SATAM) and self-assembled 3-mercaptopropionic acid monolayer (SAMPAM). The system is specific for alpha-fetoprotein and can be regenerated and reused up to 48 times. Therefore, self-assembled monolayer using thiourea which is cheaper than thioctic acid and 3-mercaptopropionic acid is a good alternative for biosensor applications when SAMs are used. (c) 2006 Elsevier B.V. All rights reserved. (Less)
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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
acid, 3-mercaptopropionic, thioctic acid, thiourea, capacitive immunosensor, self-assembled monolayer, alpha-fetoprotein
in
Biosensors & Bioelectronics
volume
22
issue
2
pages
233 - 240
publisher
Elsevier
external identifiers
  • wos:000240906100011
  • scopus:33747362376
ISSN
1873-4235
DOI
10.1016/j.bios.2005.12.025
language
English
LU publication?
yes
id
9a27af08-22bb-4fbe-8988-c5e9d33f81c3 (old id 389761)
date added to LUP
2016-04-01 17:04:03
date last changed
2020-12-08 04:17:11
@article{9a27af08-22bb-4fbe-8988-c5e9d33f81c3,
  abstract     = {A procedure was developed for the covalent coupling of anti-alpha-fetoprotein antibody (anti-AFP) to a gold surface modified with a self-assembled monolayer (SAM) of thiourea (TU). The performance of the SAM-antibody layer was compared to those of similar layers based on thioctic acid (TA) and 3-mercaptopropionic acid (MPA) by using flow injection capacitive immunosensor system. Covalent coupling of anti-AFP on self-assembled thiourea monolayer (SATUM) modified gold electrode can be used to detect alpha-fetoprotein with high efficiency, similar sensitivity, the same linear range (0.01-10 mu g l(-1)) and detection limit (10ng l(-1)) as those obtained from sensors based on self-assembled thioctic acid monolayer (SATAM) and self-assembled 3-mercaptopropionic acid monolayer (SAMPAM). The system is specific for alpha-fetoprotein and can be regenerated and reused up to 48 times. Therefore, self-assembled monolayer using thiourea which is cheaper than thioctic acid and 3-mercaptopropionic acid is a good alternative for biosensor applications when SAMs are used. (c) 2006 Elsevier B.V. All rights reserved.},
  author       = {Limbut, Warakorn and Kanatharana, Proespichaya and Mattiasson, Bo and Asawatreratanakul, Punnee and Thavarungkul, Panote},
  issn         = {1873-4235},
  language     = {eng},
  number       = {2},
  pages        = {233--240},
  publisher    = {Elsevier},
  series       = {Biosensors & Bioelectronics},
  title        = {A comparative study of capacitive immunosensors based on self-assembled monolayers formed from thiourea, thioctic acid, and 3-mercaptopropionic acid},
  url          = {http://dx.doi.org/10.1016/j.bios.2005.12.025},
  doi          = {10.1016/j.bios.2005.12.025},
  volume       = {22},
  year         = {2006},
}