A comparative study of capacitive immunosensors based on self-assembled monolayers formed from thiourea, thioctic acid, and 3-mercaptopropionic acid
(2006) In Biosensors & Bioelectronics 22(2). p.233-240- Abstract
- A procedure was developed for the covalent coupling of anti-alpha-fetoprotein antibody (anti-AFP) to a gold surface modified with a self-assembled monolayer (SAM) of thiourea (TU). The performance of the SAM-antibody layer was compared to those of similar layers based on thioctic acid (TA) and 3-mercaptopropionic acid (MPA) by using flow injection capacitive immunosensor system. Covalent coupling of anti-AFP on self-assembled thiourea monolayer (SATUM) modified gold electrode can be used to detect alpha-fetoprotein with high efficiency, similar sensitivity, the same linear range (0.01-10 mu g l(-1)) and detection limit (10ng l(-1)) as those obtained from sensors based on self-assembled thioctic acid monolayer (SATAM) and self-assembled... (More)
- A procedure was developed for the covalent coupling of anti-alpha-fetoprotein antibody (anti-AFP) to a gold surface modified with a self-assembled monolayer (SAM) of thiourea (TU). The performance of the SAM-antibody layer was compared to those of similar layers based on thioctic acid (TA) and 3-mercaptopropionic acid (MPA) by using flow injection capacitive immunosensor system. Covalent coupling of anti-AFP on self-assembled thiourea monolayer (SATUM) modified gold electrode can be used to detect alpha-fetoprotein with high efficiency, similar sensitivity, the same linear range (0.01-10 mu g l(-1)) and detection limit (10ng l(-1)) as those obtained from sensors based on self-assembled thioctic acid monolayer (SATAM) and self-assembled 3-mercaptopropionic acid monolayer (SAMPAM). The system is specific for alpha-fetoprotein and can be regenerated and reused up to 48 times. Therefore, self-assembled monolayer using thiourea which is cheaper than thioctic acid and 3-mercaptopropionic acid is a good alternative for biosensor applications when SAMs are used. (c) 2006 Elsevier B.V. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/389761
- author
- Limbut, Warakorn ; Kanatharana, Proespichaya ; Mattiasson, Bo LU ; Asawatreratanakul, Punnee and Thavarungkul, Panote
- organization
- publishing date
- 2006
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- acid, 3-mercaptopropionic, thioctic acid, thiourea, capacitive immunosensor, self-assembled monolayer, alpha-fetoprotein
- in
- Biosensors & Bioelectronics
- volume
- 22
- issue
- 2
- pages
- 233 - 240
- publisher
- Elsevier
- external identifiers
-
- wos:000240906100011
- scopus:33747362376
- ISSN
- 1873-4235
- DOI
- 10.1016/j.bios.2005.12.025
- language
- English
- LU publication?
- yes
- id
- 9a27af08-22bb-4fbe-8988-c5e9d33f81c3 (old id 389761)
- date added to LUP
- 2016-04-01 17:04:03
- date last changed
- 2022-03-07 18:16:03
@article{9a27af08-22bb-4fbe-8988-c5e9d33f81c3,
abstract = {{A procedure was developed for the covalent coupling of anti-alpha-fetoprotein antibody (anti-AFP) to a gold surface modified with a self-assembled monolayer (SAM) of thiourea (TU). The performance of the SAM-antibody layer was compared to those of similar layers based on thioctic acid (TA) and 3-mercaptopropionic acid (MPA) by using flow injection capacitive immunosensor system. Covalent coupling of anti-AFP on self-assembled thiourea monolayer (SATUM) modified gold electrode can be used to detect alpha-fetoprotein with high efficiency, similar sensitivity, the same linear range (0.01-10 mu g l(-1)) and detection limit (10ng l(-1)) as those obtained from sensors based on self-assembled thioctic acid monolayer (SATAM) and self-assembled 3-mercaptopropionic acid monolayer (SAMPAM). The system is specific for alpha-fetoprotein and can be regenerated and reused up to 48 times. Therefore, self-assembled monolayer using thiourea which is cheaper than thioctic acid and 3-mercaptopropionic acid is a good alternative for biosensor applications when SAMs are used. (c) 2006 Elsevier B.V. All rights reserved.}},
author = {{Limbut, Warakorn and Kanatharana, Proespichaya and Mattiasson, Bo and Asawatreratanakul, Punnee and Thavarungkul, Panote}},
issn = {{1873-4235}},
keywords = {{acid; 3-mercaptopropionic; thioctic acid; thiourea; capacitive immunosensor; self-assembled monolayer; alpha-fetoprotein}},
language = {{eng}},
number = {{2}},
pages = {{233--240}},
publisher = {{Elsevier}},
series = {{Biosensors & Bioelectronics}},
title = {{A comparative study of capacitive immunosensors based on self-assembled monolayers formed from thiourea, thioctic acid, and 3-mercaptopropionic acid}},
url = {{http://dx.doi.org/10.1016/j.bios.2005.12.025}},
doi = {{10.1016/j.bios.2005.12.025}},
volume = {{22}},
year = {{2006}},
}