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Relationship between PTEN gene expression and differentiation of human glioma

Yang, Y. ; Shao, N. ; Luo, G. ; Li, L. ; Nilsson-Ehle, Peter LU and Xu, Ning LU (2006) In Scandinavian Journal of Clinical & Laboratory Investigation 66(6). p.469-475
Abstract
Objective. To investigate the relationship between PTEN gene expression and differentiation of glioma. Material and methods. The quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR) method was applied to detect PTEN mRNA levels in glioma tissues. Tumor-adjacent normal tissues and benign brain tumors were used as controls. Relative PTEN mRNA levels were determined as the ratio of PTEN and GAPDH, which were correlated with the clinical pathological results. Results. PTEN mRNA levels were significantly lower in the glioma tissues than in the benign brain tumors and tumor-adjacent normal tissues, whereas there were no statistical differences between benign brain tumor and the tumor-adjacent normal tissues. According... (More)
Objective. To investigate the relationship between PTEN gene expression and differentiation of glioma. Material and methods. The quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR) method was applied to detect PTEN mRNA levels in glioma tissues. Tumor-adjacent normal tissues and benign brain tumors were used as controls. Relative PTEN mRNA levels were determined as the ratio of PTEN and GAPDH, which were correlated with the clinical pathological results. Results. PTEN mRNA levels were significantly lower in the glioma tissues than in the benign brain tumors and tumor-adjacent normal tissues, whereas there were no statistical differences between benign brain tumor and the tumor-adjacent normal tissues. According to the pathological examinations, PTEN mRNA levels were higher in the high differential glioma than the low differential glioma. Conclusions. PTEN gene expression was suppressed in the glioma, which is related to the clinical-pathological results. It is suggested that determination of PTEN mRNA levels by RT-PCR could be a novel marker of disease classification. (Less)
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author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
RT, glioma, PTEN, PCR
in
Scandinavian Journal of Clinical & Laboratory Investigation
volume
66
issue
6
pages
469 - 475
publisher
Informa Healthcare
external identifiers
  • wos:000240743500002
  • pmid:17000554
  • scopus:33749036566
ISSN
1502-7686
DOI
10.1080/00365510600763285
language
English
LU publication?
yes
id
62c8e438-9cd8-41cc-95ae-ed7fdd786cb0 (old id 389956)
date added to LUP
2016-04-01 16:47:42
date last changed
2020-01-12 19:44:48
@article{62c8e438-9cd8-41cc-95ae-ed7fdd786cb0,
  abstract     = {Objective. To investigate the relationship between PTEN gene expression and differentiation of glioma. Material and methods. The quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR) method was applied to detect PTEN mRNA levels in glioma tissues. Tumor-adjacent normal tissues and benign brain tumors were used as controls. Relative PTEN mRNA levels were determined as the ratio of PTEN and GAPDH, which were correlated with the clinical pathological results. Results. PTEN mRNA levels were significantly lower in the glioma tissues than in the benign brain tumors and tumor-adjacent normal tissues, whereas there were no statistical differences between benign brain tumor and the tumor-adjacent normal tissues. According to the pathological examinations, PTEN mRNA levels were higher in the high differential glioma than the low differential glioma. Conclusions. PTEN gene expression was suppressed in the glioma, which is related to the clinical-pathological results. It is suggested that determination of PTEN mRNA levels by RT-PCR could be a novel marker of disease classification.},
  author       = {Yang, Y. and Shao, N. and Luo, G. and Li, L. and Nilsson-Ehle, Peter and Xu, Ning},
  issn         = {1502-7686},
  language     = {eng},
  number       = {6},
  pages        = {469--475},
  publisher    = {Informa Healthcare},
  series       = {Scandinavian Journal of Clinical & Laboratory Investigation},
  title        = {Relationship between PTEN gene expression and differentiation of human glioma},
  url          = {http://dx.doi.org/10.1080/00365510600763285},
  doi          = {10.1080/00365510600763285},
  volume       = {66},
  year         = {2006},
}