Fabrication of Fragment Antibody–Enzyme Complex as a Sensing Element for Immunosensing
(2022) In International Journal of Molecular Sciences 23(3).- Abstract
Antibody–enzyme complexes (AECs) are ideal molecular recognition elements for im-munosensing applications. One molecule possesses both a binding ability to specific targets and catalytic activity to gain signals, particularly oxidoreductases, which can be integrated into rapid and sensitive electrochemical measurements. The development of AECs using fragment antibodies rather than intact antibodies, such as immunoglobulin G (IgG), has attracted attention for overcoming the ethical and cost issues associated with the production of intact antibodies. Conventionally, chemical conjugation has been used to fabricate AECs; however, controlling stoichiometric conjugation using this method is difficult. To prepare homogeneous AECs, methods... (More)
Antibody–enzyme complexes (AECs) are ideal molecular recognition elements for im-munosensing applications. One molecule possesses both a binding ability to specific targets and catalytic activity to gain signals, particularly oxidoreductases, which can be integrated into rapid and sensitive electrochemical measurements. The development of AECs using fragment antibodies rather than intact antibodies, such as immunoglobulin G (IgG), has attracted attention for overcoming the ethical and cost issues associated with the production of intact antibodies. Conventionally, chemical conjugation has been used to fabricate AECs; however, controlling stoichiometric conjugation using this method is difficult. To prepare homogeneous AECs, methods based on direct fusion and enzy-matic conjugation have been developed, and more convenient methods using Catcher/Tag systems as coupling modules have been reported. In this review, we summarize the methods for fabricating AECs using fragment antibodies developed for sensing applications and discuss the advantages and disadvantages of each method.
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- author
- Oda, Miho LU and Asano, Ryutaro
- publishing date
- 2022-02-01
- type
- Contribution to journal
- publication status
- published
- keywords
- Antibody enzyme complex, Catcher/Tag system, Chemical conjugation, Direct fusion, Enzymatic conjugation, Fragment antibody, Immunosensing, Single-chain Fv, Variable domain of heavy chain of heavy-chain antibody
- in
- International Journal of Molecular Sciences
- volume
- 23
- issue
- 3
- article number
- 1335
- publisher
- MDPI AG
- external identifiers
-
- pmid:35163258
- scopus:85123316138
- ISSN
- 1661-6596
- DOI
- 10.3390/ijms23031335
- language
- English
- LU publication?
- no
- additional info
- Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
- id
- 3899f529-eaf0-44b7-bbd7-c3fde755d131
- date added to LUP
- 2025-08-21 15:10:48
- date last changed
- 2025-11-13 23:06:26
@article{3899f529-eaf0-44b7-bbd7-c3fde755d131,
abstract = {{<p>Antibody–enzyme complexes (AECs) are ideal molecular recognition elements for im-munosensing applications. One molecule possesses both a binding ability to specific targets and catalytic activity to gain signals, particularly oxidoreductases, which can be integrated into rapid and sensitive electrochemical measurements. The development of AECs using fragment antibodies rather than intact antibodies, such as immunoglobulin G (IgG), has attracted attention for overcoming the ethical and cost issues associated with the production of intact antibodies. Conventionally, chemical conjugation has been used to fabricate AECs; however, controlling stoichiometric conjugation using this method is difficult. To prepare homogeneous AECs, methods based on direct fusion and enzy-matic conjugation have been developed, and more convenient methods using Catcher/Tag systems as coupling modules have been reported. In this review, we summarize the methods for fabricating AECs using fragment antibodies developed for sensing applications and discuss the advantages and disadvantages of each method.</p>}},
author = {{Oda, Miho and Asano, Ryutaro}},
issn = {{1661-6596}},
keywords = {{Antibody enzyme complex; Catcher/Tag system; Chemical conjugation; Direct fusion; Enzymatic conjugation; Fragment antibody; Immunosensing; Single-chain Fv; Variable domain of heavy chain of heavy-chain antibody}},
language = {{eng}},
month = {{02}},
number = {{3}},
publisher = {{MDPI AG}},
series = {{International Journal of Molecular Sciences}},
title = {{Fabrication of Fragment Antibody–Enzyme Complex as a Sensing Element for Immunosensing}},
url = {{http://dx.doi.org/10.3390/ijms23031335}},
doi = {{10.3390/ijms23031335}},
volume = {{23}},
year = {{2022}},
}