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Ecotoxicity screening of seven different types of commercial silica nanoparticles using cellular and organismic assays : Importance of surface and size

Book, Frida; Ekvall, Mikael T. LU ; Persson, Michael; Lönnerud, Sara; Lammel, Tobias; Sturve, Joachim and Backhaus, Thomas (2019) In NanoImpact 13. p.100-111
Abstract

We show that seven different types of commercial, biocide-free, colloidal silica products with mean particle sizes between 17 and 88 nm with 3 different surface chemistries (Na-stabilized, aluminized and silane-modified) are not toxic to the bacterium Pseudomonas putida, and the algae Raphidocelis subcapitata in the concentration range 5–500 mg/L. They are also not acutely toxic to Daphnia magna at concentrations up to 10,000 mg/L. Six silica particles are toxic to the gill cell line RTgill-W1 from Rainbow trout (Oncorhynchus mykiss), showing a clear concentration-response relationship with EC50 values between 13 and 92 mg/L. Toxicity in the fish cells decreases with increasing hydrodynamic size and is dependent on particle surface... (More)

We show that seven different types of commercial, biocide-free, colloidal silica products with mean particle sizes between 17 and 88 nm with 3 different surface chemistries (Na-stabilized, aluminized and silane-modified) are not toxic to the bacterium Pseudomonas putida, and the algae Raphidocelis subcapitata in the concentration range 5–500 mg/L. They are also not acutely toxic to Daphnia magna at concentrations up to 10,000 mg/L. Six silica particles are toxic to the gill cell line RTgill-W1 from Rainbow trout (Oncorhynchus mykiss), showing a clear concentration-response relationship with EC50 values between 13 and 92 mg/L. Toxicity in the fish cells decreases with increasing hydrodynamic size and is dependent on particle surface area. The average EC50 across the tested particles is 2.1 (±0.3) m2/L. Surface modifications clearly impact toxicity, with silane-modified particles showing no cytotoxicity. The reduced number of free silanol groups on the surface of the silane modified particle, in combination with an increased steric hindrance that prevents contact with the cells is a possible mechanism for the observed lack of toxicity. This is also in line with previous studies on silica nanoparticles in human toxicology. Overall, these findings show a generally low ecotoxicity of silica nanoparticles and indicate that silica particles of different sizes but identical surface chemistry could potentially be grouped into an assessment group under regulation such as REACH.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Algae, Bacteria, Colloidal nanoparticles, Concentration-response, Daphnia, Exposure metric, Fish cell line, Surface modification
in
NanoImpact
volume
13
pages
12 pages
publisher
Elsevier
external identifiers
  • scopus:85060350521
DOI
10.1016/j.impact.2019.01.001
language
English
LU publication?
yes
id
38bd4a70-6913-493e-b3bd-b04761658fe2
date added to LUP
2019-02-01 12:35:17
date last changed
2019-02-27 05:11:40
@article{38bd4a70-6913-493e-b3bd-b04761658fe2,
  abstract     = {<p>We show that seven different types of commercial, biocide-free, colloidal silica products with mean particle sizes between 17 and 88 nm with 3 different surface chemistries (Na-stabilized, aluminized and silane-modified) are not toxic to the bacterium Pseudomonas putida, and the algae Raphidocelis subcapitata in the concentration range 5–500 mg/L. They are also not acutely toxic to Daphnia magna at concentrations up to 10,000 mg/L. Six silica particles are toxic to the gill cell line RTgill-W1 from Rainbow trout (Oncorhynchus mykiss), showing a clear concentration-response relationship with EC50 values between 13 and 92 mg/L. Toxicity in the fish cells decreases with increasing hydrodynamic size and is dependent on particle surface area. The average EC50 across the tested particles is 2.1 (±0.3) m<sup>2</sup>/L. Surface modifications clearly impact toxicity, with silane-modified particles showing no cytotoxicity. The reduced number of free silanol groups on the surface of the silane modified particle, in combination with an increased steric hindrance that prevents contact with the cells is a possible mechanism for the observed lack of toxicity. This is also in line with previous studies on silica nanoparticles in human toxicology. Overall, these findings show a generally low ecotoxicity of silica nanoparticles and indicate that silica particles of different sizes but identical surface chemistry could potentially be grouped into an assessment group under regulation such as REACH.</p>},
  author       = {Book, Frida and Ekvall, Mikael T. and Persson, Michael and Lönnerud, Sara and Lammel, Tobias and Sturve, Joachim and Backhaus, Thomas},
  keyword      = {Algae,Bacteria,Colloidal nanoparticles,Concentration-response,Daphnia,Exposure metric,Fish cell line,Surface modification},
  language     = {eng},
  pages        = {100--111},
  publisher    = {Elsevier},
  series       = {NanoImpact},
  title        = {Ecotoxicity screening of seven different types of commercial silica nanoparticles using cellular and organismic assays : Importance of surface and size},
  url          = {http://dx.doi.org/10.1016/j.impact.2019.01.001},
  volume       = {13},
  year         = {2019},
}