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The effect of shaking regime on the rate and extent of enzymatic hydrolysis of cellulose

Ingesson, H; Zacchi, Guido LU ; Yang, B; Esteghlalian, AR and Saddler, JN (2001) In Journal of Biotechnology 88(2). p.177-182
Abstract
In an attempt to elucidate the effect of mixing on the rate and extent of enzymatic hydrolysis of cellulosic substrates, alpha -cellulose was hydrolysed using a commercial cellulase preparation at varying levels of substrate concentration (2.5,5 and 7.5% (w/v)) and by using three shaking regimes: continuous at low-speed (25 rpm), continuous at high-speed (150 rpm) and an intermittent regime comprised of high and low-speed shaking intervals. The continuous, high-speed shaking produced the highest conversion yields, whereas the intermittent and low-speed shaking regimes resulted in lower conversions. After 72 h, at all shaking regimes (150 rpm, 25 rpm and intermittent), using a low substrate concentration (2.5%) produced conversion yields... (More)
In an attempt to elucidate the effect of mixing on the rate and extent of enzymatic hydrolysis of cellulosic substrates, alpha -cellulose was hydrolysed using a commercial cellulase preparation at varying levels of substrate concentration (2.5,5 and 7.5% (w/v)) and by using three shaking regimes: continuous at low-speed (25 rpm), continuous at high-speed (150 rpm) and an intermittent regime comprised of high and low-speed shaking intervals. The continuous, high-speed shaking produced the highest conversion yields, whereas the intermittent and low-speed shaking regimes resulted in lower conversions. After 72 h, at all shaking regimes (150 rpm, 25 rpm and intermittent), using a low substrate concentration (2.5%) produced conversion yields (82, 79 and 80%) higher than those obtained at high (7.5%) substrate concentration (68, 63 and 68%). As the substrate concentration increased, the conversion yields at intermittent shaking gradually approached those resulting from high-speed shaking. Thus, it appears that intermittent shaking could be a beneficial process option as it can reduce the mixing energy requirements while producing reasonably high conversion yields. (C) 2001 Elsevier Science B.V. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
bioethanol, enzymatic hydrolysis, mixing, intermittent shaking, cellulose
in
Journal of Biotechnology
volume
88
issue
2
pages
177 - 182
publisher
Elsevier
external identifiers
  • wos:000169652600011
  • scopus:0035876614
ISSN
1873-4863
DOI
10.1016/S0168-1656(01)00273-5
language
English
LU publication?
yes
id
feedcc5b-9e63-4ab8-91ce-e5085ea5b665 (old id 3911289)
date added to LUP
2013-07-01 08:32:15
date last changed
2018-05-29 09:57:11
@article{feedcc5b-9e63-4ab8-91ce-e5085ea5b665,
  abstract     = {In an attempt to elucidate the effect of mixing on the rate and extent of enzymatic hydrolysis of cellulosic substrates, alpha -cellulose was hydrolysed using a commercial cellulase preparation at varying levels of substrate concentration (2.5,5 and 7.5% (w/v)) and by using three shaking regimes: continuous at low-speed (25 rpm), continuous at high-speed (150 rpm) and an intermittent regime comprised of high and low-speed shaking intervals. The continuous, high-speed shaking produced the highest conversion yields, whereas the intermittent and low-speed shaking regimes resulted in lower conversions. After 72 h, at all shaking regimes (150 rpm, 25 rpm and intermittent), using a low substrate concentration (2.5%) produced conversion yields (82, 79 and 80%) higher than those obtained at high (7.5%) substrate concentration (68, 63 and 68%). As the substrate concentration increased, the conversion yields at intermittent shaking gradually approached those resulting from high-speed shaking. Thus, it appears that intermittent shaking could be a beneficial process option as it can reduce the mixing energy requirements while producing reasonably high conversion yields. (C) 2001 Elsevier Science B.V. All rights reserved.},
  author       = {Ingesson, H and Zacchi, Guido and Yang, B and Esteghlalian, AR and Saddler, JN},
  issn         = {1873-4863},
  keyword      = {bioethanol,enzymatic hydrolysis,mixing,intermittent shaking,cellulose},
  language     = {eng},
  number       = {2},
  pages        = {177--182},
  publisher    = {Elsevier},
  series       = {Journal of Biotechnology},
  title        = {The effect of shaking regime on the rate and extent of enzymatic hydrolysis of cellulose},
  url          = {http://dx.doi.org/10.1016/S0168-1656(01)00273-5},
  volume       = {88},
  year         = {2001},
}