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Effect of acetic acid and furfural on cellulase production of Trichoderma reesei RUT C30

Szengyel, Z and Zacchi, Guido LU (2000) In Applied Biochemistry and Biotechnology 89(1). p.31-42
Abstract
Because of the high temperature applied in the steam pretreatment of lignocellulosic materials, different types of inhibiting degradation products of saccharides and lignin, such as acetic acid and furfural, are formed. The main objective of the present study was to examine the effect of acetic acid and furfural on the cellulase production of a filamentous fungus Trichoderma reesei RUT C30, which is known to be one of the best cellulase-producing strains. Mandels's mineral medium, supplemented with steam-pretreated willow as the carbon source at a concentration corresponding to 10 g/L of carbohydrate, was used. Four different concentration levels of acetic acid (0-3.0 g/L) and furfural (0-1.2 g/L) were applied alone as well as in certain... (More)
Because of the high temperature applied in the steam pretreatment of lignocellulosic materials, different types of inhibiting degradation products of saccharides and lignin, such as acetic acid and furfural, are formed. The main objective of the present study was to examine the effect of acetic acid and furfural on the cellulase production of a filamentous fungus Trichoderma reesei RUT C30, which is known to be one of the best cellulase-producing strains. Mandels's mineral medium, supplemented with steam-pretreated willow as the carbon source at a concentration corresponding to 10 g/L of carbohydrate, was used. Four different concentration levels of acetic acid (0-3.0 g/L) and furfural (0-1.2 g/L) were applied alone as well as in certain combinations. Two enzyme activities, cellulase and beta -glucosidase, were measured. The highest cellulase activity obtained after a 7-d incubation was 1.55 FPU/mL with 1.0 g/L of acetic acid and 0.8 g/L of furfural added to the medium. This was 17% higher than that obtained without acetic acid and furfural. Furthermore, the results showed that acetic acid alone did not influence the cellulase activity even at the highest concentration. However, beta -glucosidase activity was increased with increasing acetic acid concentration. Furfural proved to be an inhibiting agent causing a significant decrease in both cellulase and beta -glucosidase production. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Trichoderma reesei RUT C30, cellulase, steam-pretreated willow, inhibition, acetic acid, furfural
in
Applied Biochemistry and Biotechnology
volume
89
issue
1
pages
31 - 42
publisher
Humana Press
external identifiers
  • wos:000089949300003
  • scopus:0033792370
ISSN
1559-0291
DOI
10.1385/ABAB:89:1:31
language
English
LU publication?
yes
id
da1578bb-ca12-46d7-a668-93307b507127 (old id 3911329)
date added to LUP
2013-06-28 15:00:25
date last changed
2017-08-13 03:28:56
@article{da1578bb-ca12-46d7-a668-93307b507127,
  abstract     = {Because of the high temperature applied in the steam pretreatment of lignocellulosic materials, different types of inhibiting degradation products of saccharides and lignin, such as acetic acid and furfural, are formed. The main objective of the present study was to examine the effect of acetic acid and furfural on the cellulase production of a filamentous fungus Trichoderma reesei RUT C30, which is known to be one of the best cellulase-producing strains. Mandels's mineral medium, supplemented with steam-pretreated willow as the carbon source at a concentration corresponding to 10 g/L of carbohydrate, was used. Four different concentration levels of acetic acid (0-3.0 g/L) and furfural (0-1.2 g/L) were applied alone as well as in certain combinations. Two enzyme activities, cellulase and beta -glucosidase, were measured. The highest cellulase activity obtained after a 7-d incubation was 1.55 FPU/mL with 1.0 g/L of acetic acid and 0.8 g/L of furfural added to the medium. This was 17% higher than that obtained without acetic acid and furfural. Furthermore, the results showed that acetic acid alone did not influence the cellulase activity even at the highest concentration. However, beta -glucosidase activity was increased with increasing acetic acid concentration. Furfural proved to be an inhibiting agent causing a significant decrease in both cellulase and beta -glucosidase production.},
  author       = {Szengyel, Z and Zacchi, Guido},
  issn         = {1559-0291},
  keyword      = {Trichoderma reesei RUT C30,cellulase,steam-pretreated willow,inhibition,acetic acid,furfural},
  language     = {eng},
  number       = {1},
  pages        = {31--42},
  publisher    = {Humana Press},
  series       = {Applied Biochemistry and Biotechnology},
  title        = {Effect of acetic acid and furfural on cellulase production of Trichoderma reesei RUT C30},
  url          = {http://dx.doi.org/10.1385/ABAB:89:1:31},
  volume       = {89},
  year         = {2000},
}