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Use of holographic laser interferometry to study the diffusion of polymers in gels

Roger, P; Mattisson, C; Axelsson, Anders LU and Zacchi, Guido LU (2000) In Biotechnology and Bioengineering 69(6). p.654-663
Abstract
The aim of this study was to demonstrate the potential for holographic interferometry to be used for diffusion studies of large molecules in gels. The diffusion and partitioning of BSA (67,000 g/mol) and pullulans (5,900-112,000 g/mol) in agarose gel were investigated. The gel diffusion coefficients obtained for BSA were higher when distilled water was used as a solvent compared to those obtained with 0.1 M NaCl as the solvent. Furthermore, the gel diffusion coefficient increased with increasing BSA concentration. The same trend was found for liquid BSA diffusion coefficients obtained by DLS. BSA partition coefficients obtained at different agarose gel concentrations (2-6%, w/w) decreased slightly with increasing gel concentration.... (More)
The aim of this study was to demonstrate the potential for holographic interferometry to be used for diffusion studies of large molecules in gels. The diffusion and partitioning of BSA (67,000 g/mol) and pullulans (5,900-112,000 g/mol) in agarose gel were investigated. The gel diffusion coefficients obtained for BSA were higher when distilled water was used as a solvent compared to those obtained with 0.1 M NaCl as the solvent. Furthermore, the gel diffusion coefficient increased with increasing BSA concentration. The same trend was found for liquid BSA diffusion coefficients obtained by DLS. BSA partition coefficients obtained at different agarose gel concentrations (2-6%, w/w) decreased slightly with increasing gel concentration. However, all BSA gel diffusion coefficients measured were significantly lower than those in pure solvent and they decreased with increasing agarose concentration. The gel diffusion coefficients obtained for pullulans decreased with increasing pullulan molecular weight. The same effect from increased molecular weight was seen in the liquid diffusion coefficients measured by DLS. The pullulan partition coefficients obtained decreased with increasing molecular weight. However, pullulans with a larger Stokes' radius than BSA had partition coefficients that were higher or approximately the same as BSA. This implied that the pullulan molecules were more flexible than the BSA molecules. The results obtained for BSA in this study agreed well with other experimental studies. In addition, the magnitude of the relative standard deviation was acceptable and in the same range as for many other methods. The results thereby obtained showed that holographic interferometry is a suitable method for studying diffusion of macromolecules in gels. (C) 2000 John Wiley & Sons, Inc. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
diffusion measurement, gel, interferometry, polymer, pullulan, BSA
in
Biotechnology and Bioengineering
volume
69
issue
6
pages
654 - 663
publisher
John Wiley & Sons
external identifiers
  • wos:000089022700010
  • scopus:0034692598
ISSN
1097-0290
DOI
10.1002/1097-0290(20000920)69:6<654::AID-BIT10>3.0.CO;2-N
language
English
LU publication?
yes
id
a3acd130-d770-4636-82fd-222ccc143628 (old id 3911333)
date added to LUP
2013-07-01 08:12:11
date last changed
2017-01-01 04:51:09
@article{a3acd130-d770-4636-82fd-222ccc143628,
  abstract     = {The aim of this study was to demonstrate the potential for holographic interferometry to be used for diffusion studies of large molecules in gels. The diffusion and partitioning of BSA (67,000 g/mol) and pullulans (5,900-112,000 g/mol) in agarose gel were investigated. The gel diffusion coefficients obtained for BSA were higher when distilled water was used as a solvent compared to those obtained with 0.1 M NaCl as the solvent. Furthermore, the gel diffusion coefficient increased with increasing BSA concentration. The same trend was found for liquid BSA diffusion coefficients obtained by DLS. BSA partition coefficients obtained at different agarose gel concentrations (2-6%, w/w) decreased slightly with increasing gel concentration. However, all BSA gel diffusion coefficients measured were significantly lower than those in pure solvent and they decreased with increasing agarose concentration. The gel diffusion coefficients obtained for pullulans decreased with increasing pullulan molecular weight. The same effect from increased molecular weight was seen in the liquid diffusion coefficients measured by DLS. The pullulan partition coefficients obtained decreased with increasing molecular weight. However, pullulans with a larger Stokes' radius than BSA had partition coefficients that were higher or approximately the same as BSA. This implied that the pullulan molecules were more flexible than the BSA molecules. The results obtained for BSA in this study agreed well with other experimental studies. In addition, the magnitude of the relative standard deviation was acceptable and in the same range as for many other methods. The results thereby obtained showed that holographic interferometry is a suitable method for studying diffusion of macromolecules in gels. (C) 2000 John Wiley &amp; Sons, Inc.},
  author       = {Roger, P and Mattisson, C and Axelsson, Anders and Zacchi, Guido},
  issn         = {1097-0290},
  keyword      = {diffusion measurement,gel,interferometry,polymer,pullulan,BSA},
  language     = {eng},
  number       = {6},
  pages        = {654--663},
  publisher    = {John Wiley & Sons},
  series       = {Biotechnology and Bioengineering},
  title        = {Use of holographic laser interferometry to study the diffusion of polymers in gels},
  url          = {http://dx.doi.org/10.1002/1097-0290(20000920)69:6<654::AID-BIT10>3.0.CO;2-N},
  volume       = {69},
  year         = {2000},
}