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Simultaneous saccharification and fermentation of steam-pretreated spruce to ethanol

Bollok, M; Reczey, K and Zacchi, Guido LU (2000) In Applied Biochemistry and Biotechnology 84-6. p.69-80
Abstract
Ethanol production was studied in simultaneous saccharification and fermentation (SSF) of steam-pretreated spruce at 42 degrees C, using a thermotolerant yeast. Three yeast strains of Kluyveromyces marxianus were compared in test fermentations. SSF experiments were performed with the best of these on 5% (w/w) of substrate, at a cellulase loading of 37 filter paper units/g of cellulose, and a beta-glucosidase loading of 38 IU/ g of cellulose. The detoxification of the substrate and the lack of pH control in the experiments increased the final ethanol concentration. The final ethanol yield was 15% lower compared to SSF with Saccharomyces cerevisiae at 37 degrees C, owing to the cessation of ethanol fermentation after the first 10 h.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
simultaneous saccharification and fermentation, Kluyveromyces marxianus, ethanol fermentation, steam-pretreated spruce
in
Applied Biochemistry and Biotechnology
volume
84-6
pages
69 - 80
publisher
Humana Press
external identifiers
  • wos:000087211500007
  • scopus:0034039256
ISSN
1559-0291
DOI
10.1385/ABAB:84-86:1-9:69
language
English
LU publication?
yes
id
9957ef82-bfa1-42d7-af83-34b1db8d8ed0 (old id 3911366)
date added to LUP
2013-06-28 14:46:20
date last changed
2017-02-19 03:37:36
@article{9957ef82-bfa1-42d7-af83-34b1db8d8ed0,
  abstract     = {Ethanol production was studied in simultaneous saccharification and fermentation (SSF) of steam-pretreated spruce at 42 degrees C, using a thermotolerant yeast. Three yeast strains of Kluyveromyces marxianus were compared in test fermentations. SSF experiments were performed with the best of these on 5% (w/w) of substrate, at a cellulase loading of 37 filter paper units/g of cellulose, and a beta-glucosidase loading of 38 IU/ g of cellulose. The detoxification of the substrate and the lack of pH control in the experiments increased the final ethanol concentration. The final ethanol yield was 15% lower compared to SSF with Saccharomyces cerevisiae at 37 degrees C, owing to the cessation of ethanol fermentation after the first 10 h.},
  author       = {Bollok, M and Reczey, K and Zacchi, Guido},
  issn         = {1559-0291},
  keyword      = {simultaneous saccharification and fermentation,Kluyveromyces marxianus,ethanol fermentation,steam-pretreated spruce},
  language     = {eng},
  pages        = {69--80},
  publisher    = {Humana Press},
  series       = {Applied Biochemistry and Biotechnology},
  title        = {Simultaneous saccharification and fermentation of steam-pretreated spruce to ethanol},
  url          = {http://dx.doi.org/10.1385/ABAB:84-86:1-9:69},
  volume       = {84-6},
  year         = {2000},
}