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Cellulase production of Trichoderma reesei Rut C 30 using steam-pretreated spruce - Hydrolytic potential of cellulases on different substrates

Szengyel, Z; Zacchi, Guido LU ; Varga, A and Reczey, K (2000) In Applied Biochemistry and Biotechnology 84-6. p.679-691
Abstract
Various techniques are available for the conversion of lignocellulosics to fuel ethanol. During the last decade processes based on enzymatic hydrolysis of cellulose have been investigated more extensively, showing good yield on both hardwood and softwood. The cellulase production of a filamentous fungi, Trichoderma reesei Rut C 30, was examined on carbon sources obtained after steam pretreatment of spruce. These materials were washed fibrous steam-pretreated spruce (SPS), and hemicellulose hydrolysate. The hemicellulose hydrolysate contained, besides water-soluble carbohydrates, lignin and sugar degradation products, which were formed during the pretreatment and proved to be inhibitory to microorganisms. Experiments were performed in a 4-L... (More)
Various techniques are available for the conversion of lignocellulosics to fuel ethanol. During the last decade processes based on enzymatic hydrolysis of cellulose have been investigated more extensively, showing good yield on both hardwood and softwood. The cellulase production of a filamentous fungi, Trichoderma reesei Rut C 30, was examined on carbon sources obtained after steam pretreatment of spruce. These materials were washed fibrous steam-pretreated spruce (SPS), and hemicellulose hydrolysate. The hemicellulose hydrolysate contained, besides water-soluble carbohydrates, lignin and sugar degradation products, which were formed during the pretreatment and proved to be inhibitory to microorganisms. Experiments were performed in a 4-L laboratory fermentor. The hydrolytic capacity of the produced enzyme solutions was compared with two commercially available enzyme preparations, Celluclast and Iogen Cellulase, on SPS, washed SPS, and Solka Flee cellulose powder. There was no significant difference among the different enzymes produced by T, reesei Rut C 30. However, the conversion of cellulose using these enzymes was higher than that obtained with Iogen or Celluclast cellulases using steam-pretreated spruce as substrate. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
cellulase production, steam-pretreated spruce, enzymatic capacity of, cellulases, filter paper activity measurement, Trichoderma
in
Applied Biochemistry and Biotechnology
volume
84-6
pages
679 - 691
publisher
Humana Press
external identifiers
  • wos:000087211500056
  • scopus:0034120459
ISSN
1559-0291
DOI
10.1385/ABAB:84-86:1-9:679
language
English
LU publication?
yes
id
37e47736-0b37-47df-bdbf-5ed5e9c2904e (old id 3911371)
date added to LUP
2013-06-28 14:53:25
date last changed
2017-04-23 03:42:31
@article{37e47736-0b37-47df-bdbf-5ed5e9c2904e,
  abstract     = {Various techniques are available for the conversion of lignocellulosics to fuel ethanol. During the last decade processes based on enzymatic hydrolysis of cellulose have been investigated more extensively, showing good yield on both hardwood and softwood. The cellulase production of a filamentous fungi, Trichoderma reesei Rut C 30, was examined on carbon sources obtained after steam pretreatment of spruce. These materials were washed fibrous steam-pretreated spruce (SPS), and hemicellulose hydrolysate. The hemicellulose hydrolysate contained, besides water-soluble carbohydrates, lignin and sugar degradation products, which were formed during the pretreatment and proved to be inhibitory to microorganisms. Experiments were performed in a 4-L laboratory fermentor. The hydrolytic capacity of the produced enzyme solutions was compared with two commercially available enzyme preparations, Celluclast and Iogen Cellulase, on SPS, washed SPS, and Solka Flee cellulose powder. There was no significant difference among the different enzymes produced by T, reesei Rut C 30. However, the conversion of cellulose using these enzymes was higher than that obtained with Iogen or Celluclast cellulases using steam-pretreated spruce as substrate.},
  author       = {Szengyel, Z and Zacchi, Guido and Varga, A and Reczey, K},
  issn         = {1559-0291},
  keyword      = {cellulase production,steam-pretreated spruce,enzymatic capacity of,cellulases,filter paper activity measurement,Trichoderma},
  language     = {eng},
  pages        = {679--691},
  publisher    = {Humana Press},
  series       = {Applied Biochemistry and Biotechnology},
  title        = {Cellulase production of Trichoderma reesei Rut C 30 using steam-pretreated spruce - Hydrolytic potential of cellulases on different substrates},
  url          = {http://dx.doi.org/10.1385/ABAB:84-86:1-9:679},
  volume       = {84-6},
  year         = {2000},
}