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High-solids enzymatic hydrolysis of steam-exploded willow without prior water washing

Pristavka, A; Kodituvakky, PA; Kozlov, YP; Zacchi, Guido LU ; Berezin, IV and Rabinovich, ML (2000) In Applied Biochemistry and Microbiology 36(2). p.101-108
Abstract
A laboratory reactor equipped with a screw press was used for the hydrolysis of steam-SO2-exploded willow Salix caprea by a composition of Trichodemma reesei and Aspergillusfoetidus enzyme preparations at high substrate concentration. Optimal conditions providing the maximal volume of hydrolysis syrup with maximal sugar concentrations were determined. Two different hydrolysis procedures were developed in order to exclude the initial washing of steam-pretreated plant raw material by large volumes of water, which was necessary to eliminate the inhibitory effect of explosion byproducts on enzymatic hydrolysis. The first procedure included enzymatic prehydrolysis of the substrate for 1 h; separation of sugar syrup containing 40-60 g/l glucose,... (More)
A laboratory reactor equipped with a screw press was used for the hydrolysis of steam-SO2-exploded willow Salix caprea by a composition of Trichodemma reesei and Aspergillusfoetidus enzyme preparations at high substrate concentration. Optimal conditions providing the maximal volume of hydrolysis syrup with maximal sugar concentrations were determined. Two different hydrolysis procedures were developed in order to exclude the initial washing of steam-pretreated plant raw material by large volumes of water, which was necessary to eliminate the inhibitory effect of explosion byproducts on enzymatic hydrolysis. The first procedure included enzymatic prehydrolysis of the substrate for 1 h; separation of sugar syrup containing 40-60 g/l glucose, 20-25 g/l xylose, and up to 10 g/l disaccharides, as well as up to 35% of the initial enzymatic activity; then addition of a diluted acetate buffer (pH 4.5); and subsequent hydrolysis of the substrate by the adsorbed enzymes leading to the final accumulation of up to 140 g/l glucose and up to 15 g/l of xylose. In the second scenario, the exploded willow was initially adjusted by alkali to pH 4.5 and then hydrolyzed directly by the added enzymes over 24 h. This Procedure resulted in a nearly total polysaccharide hydrolysis and accumulation of up to 170 g/l glucose and 20 g/l xylose. The reasons for inhibition of enzymatic hydrolysis are discussed. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Applied Biochemistry and Microbiology
volume
36
issue
2
pages
101 - 108
publisher
MAIK Nauka/Interperiodica
external identifiers
  • wos:000085919800002
  • scopus:0034148173
ISSN
0003-6838
DOI
10.1007/BF02737902
language
English
LU publication?
yes
id
070197c8-3d98-43ae-b84c-d1c4f78fbc25 (old id 3911377)
date added to LUP
2013-06-28 14:57:41
date last changed
2017-01-01 06:56:20
@article{070197c8-3d98-43ae-b84c-d1c4f78fbc25,
  abstract     = {A laboratory reactor equipped with a screw press was used for the hydrolysis of steam-SO2-exploded willow Salix caprea by a composition of Trichodemma reesei and Aspergillusfoetidus enzyme preparations at high substrate concentration. Optimal conditions providing the maximal volume of hydrolysis syrup with maximal sugar concentrations were determined. Two different hydrolysis procedures were developed in order to exclude the initial washing of steam-pretreated plant raw material by large volumes of water, which was necessary to eliminate the inhibitory effect of explosion byproducts on enzymatic hydrolysis. The first procedure included enzymatic prehydrolysis of the substrate for 1 h; separation of sugar syrup containing 40-60 g/l glucose, 20-25 g/l xylose, and up to 10 g/l disaccharides, as well as up to 35% of the initial enzymatic activity; then addition of a diluted acetate buffer (pH 4.5); and subsequent hydrolysis of the substrate by the adsorbed enzymes leading to the final accumulation of up to 140 g/l glucose and up to 15 g/l of xylose. In the second scenario, the exploded willow was initially adjusted by alkali to pH 4.5 and then hydrolyzed directly by the added enzymes over 24 h. This Procedure resulted in a nearly total polysaccharide hydrolysis and accumulation of up to 170 g/l glucose and 20 g/l xylose. The reasons for inhibition of enzymatic hydrolysis are discussed.},
  author       = {Pristavka, A and Kodituvakky, PA and Kozlov, YP and Zacchi, Guido and Berezin, IV and Rabinovich, ML},
  issn         = {0003-6838},
  language     = {eng},
  number       = {2},
  pages        = {101--108},
  publisher    = {MAIK Nauka/Interperiodica},
  series       = {Applied Biochemistry and Microbiology},
  title        = {High-solids enzymatic hydrolysis of steam-exploded willow without prior water washing},
  url          = {http://dx.doi.org/10.1007/BF02737902},
  volume       = {36},
  year         = {2000},
}