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The influence of lactic acid formation on the simultaneous saccharification and fermentation (SSF) of softwood to ethanol

Stenberg, K; Galbe, Mats LU and Zacchi, Guido LU (2000) In Enzyme and Microbial Technology 26(1). p.71-79
Abstract
Simultaneous saccharification and fermentation of wood to ethanol has often been claimed to be less sensitive to infection than separate hydrolysis and fermentation. A previous study has shown that this was not the case. In the present study the influence of different parameters in SSF, such as cell concentration, nutrient composition and inclusion of the liquid from pretreatment, on the ethanol yield and lactic acid formation was investigated for steam-pretreated softwood. Commercial enzymes from Novo and Saccharomyces cerevisiae, in the form of compressed baker's yeast, were used for hydrolysis and fermentation. The formation of lactic acid was much higher when washed substrate instead of whole slurry from the pretreatment stage was used... (More)
Simultaneous saccharification and fermentation of wood to ethanol has often been claimed to be less sensitive to infection than separate hydrolysis and fermentation. A previous study has shown that this was not the case. In the present study the influence of different parameters in SSF, such as cell concentration, nutrient composition and inclusion of the liquid from pretreatment, on the ethanol yield and lactic acid formation was investigated for steam-pretreated softwood. Commercial enzymes from Novo and Saccharomyces cerevisiae, in the form of compressed baker's yeast, were used for hydrolysis and fermentation. The formation of lactic acid was much higher when washed substrate instead of whole slurry from the pretreatment stage was used in SSF. The addition of extra nutrients, such as yeast extract, delayed lactic acid formation, whereas the addition of casamino acids stimulated lactic acid formation. The liquid from the pretreatment stage had a positive effect on the ethanol yield in SSF, as well as a reducing effect on the lactic acid formation. (C) 2000 Elsevier Science Inc. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
SSF, Saccharomyces cerevisiae, lactic acid formation, ethanol yield, infection, softwood
in
Enzyme and Microbial Technology
volume
26
issue
1
pages
71 - 79
publisher
Elsevier
external identifiers
  • wos:000085135800009
  • scopus:0033965157
ISSN
0141-0229
DOI
10.1016/S0141-0229(99)00127-1
language
English
LU publication?
yes
id
240a7d08-043f-4998-ad06-32413c305e2d (old id 3911385)
date added to LUP
2013-06-28 14:59:05
date last changed
2017-04-02 03:37:14
@article{240a7d08-043f-4998-ad06-32413c305e2d,
  abstract     = {Simultaneous saccharification and fermentation of wood to ethanol has often been claimed to be less sensitive to infection than separate hydrolysis and fermentation. A previous study has shown that this was not the case. In the present study the influence of different parameters in SSF, such as cell concentration, nutrient composition and inclusion of the liquid from pretreatment, on the ethanol yield and lactic acid formation was investigated for steam-pretreated softwood. Commercial enzymes from Novo and Saccharomyces cerevisiae, in the form of compressed baker's yeast, were used for hydrolysis and fermentation. The formation of lactic acid was much higher when washed substrate instead of whole slurry from the pretreatment stage was used in SSF. The addition of extra nutrients, such as yeast extract, delayed lactic acid formation, whereas the addition of casamino acids stimulated lactic acid formation. The liquid from the pretreatment stage had a positive effect on the ethanol yield in SSF, as well as a reducing effect on the lactic acid formation. (C) 2000 Elsevier Science Inc. All rights reserved.},
  author       = {Stenberg, K and Galbe, Mats and Zacchi, Guido},
  issn         = {0141-0229},
  keyword      = {SSF,Saccharomyces cerevisiae,lactic acid formation,ethanol yield,infection,softwood},
  language     = {eng},
  number       = {1},
  pages        = {71--79},
  publisher    = {Elsevier},
  series       = {Enzyme and Microbial Technology},
  title        = {The influence of lactic acid formation on the simultaneous saccharification and fermentation (SSF) of softwood to ethanol},
  url          = {http://dx.doi.org/10.1016/S0141-0229(99)00127-1},
  volume       = {26},
  year         = {2000},
}