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Simulation of chromatographic processes applied to separation of proteins

Kempe, Henrik LU ; Axelsson, Anders LU ; Nilssen, B and Zacchi, Guido LU (1999) In Journal of Chromatography A 846(1-2). p.1-12
Abstract
The advantages of using a detailed mathematical model for fixed bed chromatography is demonstrated by the personal computer program SIMCHROM. The chromatography model includes axial dispersion in the bulk liquid, external and internal mass transfer resistances and an instationary non-linear adsorption model. Frontal and pulse chromatography can be studied for single and multicomponent systems. The simulation program can easily be used to make parametric evaluations to study the influence of variations in physical, kinetical and operating parameters. The special features of the present intrinsic model is demonstrated by comparing the SIMCHROM results With simulations using simplified lumped models. Experimental data describing affinity... (More)
The advantages of using a detailed mathematical model for fixed bed chromatography is demonstrated by the personal computer program SIMCHROM. The chromatography model includes axial dispersion in the bulk liquid, external and internal mass transfer resistances and an instationary non-linear adsorption model. Frontal and pulse chromatography can be studied for single and multicomponent systems. The simulation program can easily be used to make parametric evaluations to study the influence of variations in physical, kinetical and operating parameters. The special features of the present intrinsic model is demonstrated by comparing the SIMCHROM results With simulations using simplified lumped models. Experimental data describing affinity chromatography of lysozyme on Cibacron Blue Sepharose CL-6B is used as a model system. The intrinsic model is able to describe variations in the physical, kinetic and operating parameters better than the simplified models. This results in a more reliable prediction of the performance of the chromatography process as well as a better understanding of che underlying mechanisms responsible for the separation. (C) 1999 Elsevier Science B.V. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
mathematical modelling, computer simulation, proteins
in
Journal of Chromatography A
volume
846
issue
1-2
pages
1 - 12
publisher
Elsevier
external identifiers
  • wos:000081113900001
  • scopus:0032791201
ISSN
0021-9673
DOI
10.1016/S0021-9673(98)01079-6
language
English
LU publication?
yes
id
cc2f5184-590f-4f4c-a26a-6eef02f0dbf8 (old id 3911399)
date added to LUP
2013-06-28 14:36:40
date last changed
2017-04-09 04:20:09
@article{cc2f5184-590f-4f4c-a26a-6eef02f0dbf8,
  abstract     = {The advantages of using a detailed mathematical model for fixed bed chromatography is demonstrated by the personal computer program SIMCHROM. The chromatography model includes axial dispersion in the bulk liquid, external and internal mass transfer resistances and an instationary non-linear adsorption model. Frontal and pulse chromatography can be studied for single and multicomponent systems. The simulation program can easily be used to make parametric evaluations to study the influence of variations in physical, kinetical and operating parameters. The special features of the present intrinsic model is demonstrated by comparing the SIMCHROM results With simulations using simplified lumped models. Experimental data describing affinity chromatography of lysozyme on Cibacron Blue Sepharose CL-6B is used as a model system. The intrinsic model is able to describe variations in the physical, kinetic and operating parameters better than the simplified models. This results in a more reliable prediction of the performance of the chromatography process as well as a better understanding of che underlying mechanisms responsible for the separation. (C) 1999 Elsevier Science B.V. All rights reserved.},
  author       = {Kempe, Henrik and Axelsson, Anders and Nilssen, B and Zacchi, Guido},
  issn         = {0021-9673},
  keyword      = {mathematical modelling,computer simulation,proteins},
  language     = {eng},
  number       = {1-2},
  pages        = {1--12},
  publisher    = {Elsevier},
  series       = {Journal of Chromatography A},
  title        = {Simulation of chromatographic processes applied to separation of proteins},
  url          = {http://dx.doi.org/10.1016/S0021-9673(98)01079-6},
  volume       = {846},
  year         = {1999},
}