Advanced

Use of hemicellulose hydrolysate for beta-glucosidase fermentation

Reczey, K; Brumbauer, A; Bollok, M; Szengyel, Z and Zacchi, Guido LU (1998) In Applied Biochemistry and Biotechnology 70-2. p.225-235
Abstract
Hydrolysis of cellulose by Trichoderma cellulases often results in a mixture of glucose, cellobiose, and low-mol-wt cellodextrins. Cellobiose is nonfermentable for most yeasts, and therefore it has to be hydrolyzed to glucose by beta-glucosidase prior to ethanol fermentation. In the present study, the beta-glucosidase production of one Penicillium and three Aspergillus strains, which were previously selected out of 24 strains, was investigated on steam pretreated willow. Both steam-pretreated willow and hemicellulose hydrolysate, released during steam explosion of willow, were used as carbon sources. Reference cultivation runs were performed using prehydrolyzed Solka Flee and glucose. The four strains were compared with Trichoderma reesei... (More)
Hydrolysis of cellulose by Trichoderma cellulases often results in a mixture of glucose, cellobiose, and low-mol-wt cellodextrins. Cellobiose is nonfermentable for most yeasts, and therefore it has to be hydrolyzed to glucose by beta-glucosidase prior to ethanol fermentation. In the present study, the beta-glucosidase production of one Penicillium and three Aspergillus strains, which were previously selected out of 24 strains, was investigated on steam pretreated willow. Both steam-pretreated willow and hemicellulose hydrolysate, released during steam explosion of willow, were used as carbon sources. Reference cultivation runs were performed using prehydrolyzed Solka Flee and glucose. The four strains were compared with Trichoderma reesei regarding sugar consumption and beta-glucosidase production. Aspergillus niger and Aspergillus phoenicis proved to be the best enzyme producers on hemicellulose hydrolysate. The maximum beta-glucosidase activity, 4.60 IU/mL, was obtained when A. phoenicis was cultivated on the mixture of hemicellulose hydrolysate and steam-pretreated willow. The maximum yield of enzyme activity, 502 IU/g total carbohydrate, was obtained when Aspergillus foetidus was cultivated on the hemicellulose hydrolysate. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
beta-glucosidase production, hemicellulose hydrolysate of willow, Trichoderma, Penicillium, Aspergillus
in
Applied Biochemistry and Biotechnology
volume
70-2
pages
225 - 235
publisher
Humana Press
external identifiers
  • wos:000073769000023
  • scopus:5544245106
ISSN
1559-0291
DOI
10.1007/BF02920139
language
English
LU publication?
yes
id
ad0cd342-5cbb-4c85-a2cf-4cfa786054e4 (old id 3911444)
date added to LUP
2013-06-28 14:15:43
date last changed
2017-07-02 03:28:36
@article{ad0cd342-5cbb-4c85-a2cf-4cfa786054e4,
  abstract     = {Hydrolysis of cellulose by Trichoderma cellulases often results in a mixture of glucose, cellobiose, and low-mol-wt cellodextrins. Cellobiose is nonfermentable for most yeasts, and therefore it has to be hydrolyzed to glucose by beta-glucosidase prior to ethanol fermentation. In the present study, the beta-glucosidase production of one Penicillium and three Aspergillus strains, which were previously selected out of 24 strains, was investigated on steam pretreated willow. Both steam-pretreated willow and hemicellulose hydrolysate, released during steam explosion of willow, were used as carbon sources. Reference cultivation runs were performed using prehydrolyzed Solka Flee and glucose. The four strains were compared with Trichoderma reesei regarding sugar consumption and beta-glucosidase production. Aspergillus niger and Aspergillus phoenicis proved to be the best enzyme producers on hemicellulose hydrolysate. The maximum beta-glucosidase activity, 4.60 IU/mL, was obtained when A. phoenicis was cultivated on the mixture of hemicellulose hydrolysate and steam-pretreated willow. The maximum yield of enzyme activity, 502 IU/g total carbohydrate, was obtained when Aspergillus foetidus was cultivated on the hemicellulose hydrolysate.},
  author       = {Reczey, K and Brumbauer, A and Bollok, M and Szengyel, Z and Zacchi, Guido},
  issn         = {1559-0291},
  keyword      = {beta-glucosidase production,hemicellulose hydrolysate of willow,Trichoderma,Penicillium,Aspergillus},
  language     = {eng},
  pages        = {225--235},
  publisher    = {Humana Press},
  series       = {Applied Biochemistry and Biotechnology},
  title        = {Use of hemicellulose hydrolysate for beta-glucosidase fermentation},
  url          = {http://dx.doi.org/10.1007/BF02920139},
  volume       = {70-2},
  year         = {1998},
}