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Cellulase production based on hemicellulose hydrolysate from steam-pretreated willow

Szengyel, Z; Zacchi, Guido LU and Reczey, K (1997) In Applied Biochemistry and Biotechnology 63-5. p.351-362
Abstract
The production cost of cellulolytic enzymes is a major contributor to the high cost of ethanol production from lignocellulosics using enzymatic hydrolysis. The aim of the present study was to investigate the cellulolytic enzyme production of Trichoderma reesei Rut C 30, which is known as a good cellulase secreting micro-organism, using willow as the carbon source. The willow, which is a fast-growing energy crop in Sweden, was impregnated with 1-4% SO2 and steam-pretreated for 5 min at 206 degrees C. The pretreated willow was washed and the wash water, which contains several soluble sugars from the hemicellulose, was supplemented with fibrous pretreated willow and used for enzyme production. In addition to sugars, the liquid contains... (More)
The production cost of cellulolytic enzymes is a major contributor to the high cost of ethanol production from lignocellulosics using enzymatic hydrolysis. The aim of the present study was to investigate the cellulolytic enzyme production of Trichoderma reesei Rut C 30, which is known as a good cellulase secreting micro-organism, using willow as the carbon source. The willow, which is a fast-growing energy crop in Sweden, was impregnated with 1-4% SO2 and steam-pretreated for 5 min at 206 degrees C. The pretreated willow was washed and the wash water, which contains several soluble sugars from the hemicellulose, was supplemented with fibrous pretreated willow and used for enzyme production. In addition to sugars, the liquid contains degradation products such as acetic acid, furfural, and 5-hydroxy-methylfurfural, which are inhibitory for microorganisms. The results showed that 50% of the cellulose can be replaced with sugars from the wash water. The highest enzyme activity, 1.79 FPU/mL and yield, 133 FPU/g carbohydrate, was obtained at pH 6.0 using 20 g/L carbon source concentration. At lower pHs, a total lack of growth and enzyme production was observed, which probably could be explained by furfural inhibition. (Less)
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author
organization
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Contribution to journal
publication status
published
subject
keywords
cellulase enzyme production, Trichoderma reesei Rut C 30, lignocellulosics, furfural inhibition
in
Applied Biochemistry and Biotechnology
volume
63-5
pages
351 - 362
publisher
Humana Press
external identifiers
  • wos:A1997XB48300032
  • scopus:0001408419
ISSN
1559-0291
DOI
10.1007/BF02920437
language
English
LU publication?
yes
id
7a5e44ee-fb37-4ea7-bb86-154985a9955e (old id 3911487)
date added to LUP
2013-06-28 14:00:13
date last changed
2017-01-01 04:34:42
@article{7a5e44ee-fb37-4ea7-bb86-154985a9955e,
  abstract     = {The production cost of cellulolytic enzymes is a major contributor to the high cost of ethanol production from lignocellulosics using enzymatic hydrolysis. The aim of the present study was to investigate the cellulolytic enzyme production of Trichoderma reesei Rut C 30, which is known as a good cellulase secreting micro-organism, using willow as the carbon source. The willow, which is a fast-growing energy crop in Sweden, was impregnated with 1-4% SO2 and steam-pretreated for 5 min at 206 degrees C. The pretreated willow was washed and the wash water, which contains several soluble sugars from the hemicellulose, was supplemented with fibrous pretreated willow and used for enzyme production. In addition to sugars, the liquid contains degradation products such as acetic acid, furfural, and 5-hydroxy-methylfurfural, which are inhibitory for microorganisms. The results showed that 50% of the cellulose can be replaced with sugars from the wash water. The highest enzyme activity, 1.79 FPU/mL and yield, 133 FPU/g carbohydrate, was obtained at pH 6.0 using 20 g/L carbon source concentration. At lower pHs, a total lack of growth and enzyme production was observed, which probably could be explained by furfural inhibition.},
  author       = {Szengyel, Z and Zacchi, Guido and Reczey, K},
  issn         = {1559-0291},
  keyword      = {cellulase enzyme production,Trichoderma reesei Rut C 30,lignocellulosics,furfural inhibition},
  language     = {eng},
  pages        = {351--362},
  publisher    = {Humana Press},
  series       = {Applied Biochemistry and Biotechnology},
  title        = {Cellulase production based on hemicellulose hydrolysate from steam-pretreated willow},
  url          = {http://dx.doi.org/10.1007/BF02920437},
  volume       = {63-5},
  year         = {1997},
}