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Determination of the pore-size distribution in gels

Andersson, M; Axelsson, Anders LU and Zacchi, Guido LU (1995) In Bioseparation 5(2). p.65-72
Abstract
A method for determination of the accessible volume fraction in gels as function of the molecular weight of the solutes is presented. The pore-size distribution is determined by measuring the rate of diffusion of a mixture of solutes into a gel using gel filtration for separation. The solutes, of various sizes, are detected by refractive index measurements. Two marker molecules (blue dextran and glucose) were used to determine the gel void and the amount of liquid adhering to the surface. The technique is simple and can easily be adapted to other systems of a porous nature (membranes, catalyst pellets etc.). The method is applied to an N-isopropylacrylamide gel. This gel is sensitive to temperature changes. A considerable increase in... (More)
A method for determination of the accessible volume fraction in gels as function of the molecular weight of the solutes is presented. The pore-size distribution is determined by measuring the rate of diffusion of a mixture of solutes into a gel using gel filtration for separation. The solutes, of various sizes, are detected by refractive index measurements. Two marker molecules (blue dextran and glucose) were used to determine the gel void and the amount of liquid adhering to the surface. The technique is simple and can easily be adapted to other systems of a porous nature (membranes, catalyst pellets etc.). The method is applied to an N-isopropylacrylamide gel. This gel is sensitive to temperature changes. A considerable increase in volume is obtained when the temperature is decreased. This makes it suitable for use as a separation agent in gel extraction. In order to assess the performance of this unit operation the pore size distribution for the N-isopropylacrylamide gel was determined at 10 degrees C, 20 degrees C and 30 degrees C, using mixtures of different dextrans as well as different polyethylene glycols. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
GEL FILTRATION, IN-DIFFUSION, N-ISOPROPYLACRYLAMIDE GEL, PORE-SIZE, DISTRIBUTION
in
Bioseparation
volume
5
issue
2
pages
65 - 72
publisher
Springer
external identifiers
  • wos:A1995QZ10500001
  • scopus:0029158820
ISSN
1573-8272
language
English
LU publication?
yes
id
6631db76-6a2b-4fe6-a6c6-1800ccd7e1cf (old id 3911542)
date added to LUP
2013-06-28 13:06:40
date last changed
2017-07-30 04:29:13
@article{6631db76-6a2b-4fe6-a6c6-1800ccd7e1cf,
  abstract     = {A method for determination of the accessible volume fraction in gels as function of the molecular weight of the solutes is presented. The pore-size distribution is determined by measuring the rate of diffusion of a mixture of solutes into a gel using gel filtration for separation. The solutes, of various sizes, are detected by refractive index measurements. Two marker molecules (blue dextran and glucose) were used to determine the gel void and the amount of liquid adhering to the surface. The technique is simple and can easily be adapted to other systems of a porous nature (membranes, catalyst pellets etc.). The method is applied to an N-isopropylacrylamide gel. This gel is sensitive to temperature changes. A considerable increase in volume is obtained when the temperature is decreased. This makes it suitable for use as a separation agent in gel extraction. In order to assess the performance of this unit operation the pore size distribution for the N-isopropylacrylamide gel was determined at 10 degrees C, 20 degrees C and 30 degrees C, using mixtures of different dextrans as well as different polyethylene glycols.},
  author       = {Andersson, M and Axelsson, Anders and Zacchi, Guido},
  issn         = {1573-8272},
  keyword      = {GEL FILTRATION,IN-DIFFUSION,N-ISOPROPYLACRYLAMIDE GEL,PORE-SIZE,DISTRIBUTION},
  language     = {eng},
  number       = {2},
  pages        = {65--72},
  publisher    = {Springer},
  series       = {Bioseparation},
  title        = {Determination of the pore-size distribution in gels},
  volume       = {5},
  year         = {1995},
}