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Mathematical modelling and parametric studies of affinity chromatography

Carlsson, F; Axelsson, Anders LU and Zacchi, Guido LU (1994) In Computers & Chemical Engineering 18(suppl. 1). p.657-661
Abstract
In this study a model based on mass transfer and sorption rate constants with physical significance is used for simulation of affinity chromatography, The simulation program has been used to perform a parametric analysis of the adsorption of lysozyme on Cibacron Blue-Sepharose CL-6B. The influence of process parameters as well as physical parameters on the chromatography process was investigated. The external and the internal mass transfer as well as the sorption rate were found to contribute to the control of the overall rate of the system. The most important process parameter was the radius of the beads. The optimum bead size was found to be around 50 mu m.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
AFFINITY CHROMATOGRAPHY, ION EXCHANGE-CHROMATOGRAPHY, SIMULATION, MODELING, LYSOZYME
in
Computers & Chemical Engineering
volume
18
issue
suppl. 1
pages
657 - 661
publisher
Elsevier
external identifiers
  • wos:A1994MM09800107
  • scopus:0028192850
ISSN
1873-4375
DOI
10.1016/0098-1354(94)80107-X
language
English
LU publication?
yes
id
ee759856-b35b-4fb0-9262-26ef45f4e569 (old id 3911567)
date added to LUP
2013-06-28 12:04:27
date last changed
2017-01-01 07:13:08
@article{ee759856-b35b-4fb0-9262-26ef45f4e569,
  abstract     = {In this study a model based on mass transfer and sorption rate constants with physical significance is used for simulation of affinity chromatography, The simulation program has been used to perform a parametric analysis of the adsorption of lysozyme on Cibacron Blue-Sepharose CL-6B. The influence of process parameters as well as physical parameters on the chromatography process was investigated. The external and the internal mass transfer as well as the sorption rate were found to contribute to the control of the overall rate of the system. The most important process parameter was the radius of the beads. The optimum bead size was found to be around 50 mu m.},
  author       = {Carlsson, F and Axelsson, Anders and Zacchi, Guido},
  issn         = {1873-4375},
  keyword      = {AFFINITY CHROMATOGRAPHY,ION EXCHANGE-CHROMATOGRAPHY,SIMULATION,MODELING,LYSOZYME},
  language     = {eng},
  number       = {suppl. 1},
  pages        = {657--661},
  publisher    = {Elsevier},
  series       = {Computers & Chemical Engineering},
  title        = {Mathematical modelling and parametric studies of affinity chromatography},
  url          = {http://dx.doi.org/10.1016/0098-1354(94)80107-X},
  volume       = {18},
  year         = {1994},
}