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Measurement of diffusion coefficients in gels using holographic laser interferometry

Gustafsson, N O; Westrin, B; Axelsson, Anders LU and Zacchi, Guido LU (1993) In Biotechnology Progress 9(4). p.436-441
Abstract
The accuracy and precision of holographic interferometry as a method to measure diffusion coefficients in gels are investigated both experimentally and theoretically. The standard deviations in the experimentally determined diffusion coefficient for ethanol in 4% (w/v) agarose gel were 3.3% for diffusion into the gel and 6.1% for diffusion out of the gel. These are in good agreement with the standard deviations obtained using Monte Carlo simulations. Systematic errors derived from an assumption of constant diffusion coefficients were also investigated.
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biotechnology Progress
volume
9
issue
4
pages
436 - 441
publisher
The American Chemical Society
external identifiers
  • wos:A1993LV09200012
  • scopus:0027628303
ISSN
1520-6033
DOI
10.1021/bp00022a011
language
English
LU publication?
yes
id
af25f757-ab8e-4de6-be2b-3cdb61d5dd07 (old id 3911576)
date added to LUP
2013-06-28 12:10:43
date last changed
2017-08-27 05:10:44
@article{af25f757-ab8e-4de6-be2b-3cdb61d5dd07,
  abstract     = {The accuracy and precision of holographic interferometry as a method to measure diffusion coefficients in gels are investigated both experimentally and theoretically. The standard deviations in the experimentally determined diffusion coefficient for ethanol in 4% (w/v) agarose gel were 3.3% for diffusion into the gel and 6.1% for diffusion out of the gel. These are in good agreement with the standard deviations obtained using Monte Carlo simulations. Systematic errors derived from an assumption of constant diffusion coefficients were also investigated.},
  author       = {Gustafsson, N O and Westrin, B and Axelsson, Anders and Zacchi, Guido},
  issn         = {1520-6033},
  language     = {eng},
  number       = {4},
  pages        = {436--441},
  publisher    = {The American Chemical Society},
  series       = {Biotechnology Progress},
  title        = {Measurement of diffusion coefficients in gels using holographic laser interferometry},
  url          = {http://dx.doi.org/10.1021/bp00022a011},
  volume       = {9},
  year         = {1993},
}