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Adsorption of cellulases on steam-pretreated willow

Galbe, Mats LU ; Eklund, R and Zacchi, Guido LU (1990) In Applied Biochemistry and Biotechnology 24-5. p.87-101
Abstract
The adsorption of cellulases on steam-pretreated willow has been measured for2, 4, and 8 wt% willow and with varying enzyme concentrations (2–100 wt% based on substrate). The enzyme concentration was measured as soluble protein, filter paper activity (FPA), CMC-ase activity, and activity toward willow. The adsorption data were modeled with a Langmuir isotherm. The maximum adsorption capability depends on the method for measurement of the enzyme concentration. The lowest value, 470 mg enzyme/g willow, was obtained for the soluble protein and the highest value, 650 mg/g, for the FPA. For technical applications, a single isotherm can be used for calculation of the adsorption capability of steam-treated willow.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Applied Biochemistry and Biotechnology
volume
24-5
pages
87 - 101
publisher
Humana Press
external identifiers
  • wos:A1990DE57300010
  • scopus:0025148108
ISSN
1559-0291
DOI
10.1007/BF02920236
language
English
LU publication?
yes
id
b82c2434-ea0d-4bd3-9ccf-e68aa4cc8b77 (old id 3911606)
date added to LUP
2013-06-28 10:49:49
date last changed
2017-01-01 04:28:26
@article{b82c2434-ea0d-4bd3-9ccf-e68aa4cc8b77,
  abstract     = {The adsorption of cellulases on steam-pretreated willow has been measured for2, 4, and 8 wt% willow and with varying enzyme concentrations (2–100 wt% based on substrate). The enzyme concentration was measured as soluble protein, filter paper activity (FPA), CMC-ase activity, and activity toward willow. The adsorption data were modeled with a Langmuir isotherm. The maximum adsorption capability depends on the method for measurement of the enzyme concentration. The lowest value, 470 mg enzyme/g willow, was obtained for the soluble protein and the highest value, 650 mg/g, for the FPA. For technical applications, a single isotherm can be used for calculation of the adsorption capability of steam-treated willow.},
  author       = {Galbe, Mats and Eklund, R and Zacchi, Guido},
  issn         = {1559-0291},
  language     = {eng},
  pages        = {87--101},
  publisher    = {Humana Press},
  series       = {Applied Biochemistry and Biotechnology},
  title        = {Adsorption of cellulases on steam-pretreated willow},
  url          = {http://dx.doi.org/10.1007/BF02920236},
  volume       = {24-5},
  year         = {1990},
}