Optimization of temperature and enzyme concentration in the enzymatic saccharification of steam-pretreated willow
(1990) In Enzyme and Microbial Technology 12(3). p.225-228- Abstract
- The effect of temperature and enzyme concentration on the enzymatic hydrolysis of steam-pretreated willow was investigated to obtain the optimal hydrolysis conditions. The willow was pretreated at 220°C for 10 min according to the results of earlier optimization studies of the steam-pretreatment conditions for high glucose yields. The concentrations of two commercial enzymes, Celluclast 2L and Novozyme 188, were varied in the range 0–20% (based on oven-dry substrate). A maximum in the conversion of cellulose to glucose was obtained using a mixture of 15% Celluclast and 3% Novozyme, corresponding to an activity of 11.4 FPU g−1 substrate. Higher enzyme concentrations did not increase the initial hydrolysis rate nor affect the final glucose... (More)
- The effect of temperature and enzyme concentration on the enzymatic hydrolysis of steam-pretreated willow was investigated to obtain the optimal hydrolysis conditions. The willow was pretreated at 220°C for 10 min according to the results of earlier optimization studies of the steam-pretreatment conditions for high glucose yields. The concentrations of two commercial enzymes, Celluclast 2L and Novozyme 188, were varied in the range 0–20% (based on oven-dry substrate). A maximum in the conversion of cellulose to glucose was obtained using a mixture of 15% Celluclast and 3% Novozyme, corresponding to an activity of 11.4 FPU g−1 substrate. Higher enzyme concentrations did not increase the initial hydrolysis rate nor affect the final glucose yield. The effects of temperature in the range 40°C to 60°C were investigated. The temperature affects both the initial hydrolysis rate and the final glucose yield. The highest glucose yield was obtained at 40°C. The yield decreased with increasing temperature, due to increased enzyme deactivation at higher temperatures, while the initial reaction rate increased with increasing temperatures. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3911615
- author
- Eklund, R ; Galbe, Mats LU and Zacchi, Guido LU
- organization
- publishing date
- 1990
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Enzyme and Microbial Technology
- volume
- 12
- issue
- 3
- pages
- 225 - 228
- publisher
- Elsevier
- external identifiers
-
- wos:A1990CN67700011
- scopus:0025400283
- ISSN
- 0141-0229
- DOI
- 10.1016/0141-0229(90)90043-P
- language
- English
- LU publication?
- yes
- id
- f06f314e-f144-4c7d-92c0-43ca4c548fe1 (old id 3911615)
- date added to LUP
- 2016-04-01 12:06:33
- date last changed
- 2023-11-11 13:11:51
@article{f06f314e-f144-4c7d-92c0-43ca4c548fe1,
abstract = {{The effect of temperature and enzyme concentration on the enzymatic hydrolysis of steam-pretreated willow was investigated to obtain the optimal hydrolysis conditions. The willow was pretreated at 220°C for 10 min according to the results of earlier optimization studies of the steam-pretreatment conditions for high glucose yields. The concentrations of two commercial enzymes, Celluclast 2L and Novozyme 188, were varied in the range 0–20% (based on oven-dry substrate). A maximum in the conversion of cellulose to glucose was obtained using a mixture of 15% Celluclast and 3% Novozyme, corresponding to an activity of 11.4 FPU g−1 substrate. Higher enzyme concentrations did not increase the initial hydrolysis rate nor affect the final glucose yield. The effects of temperature in the range 40°C to 60°C were investigated. The temperature affects both the initial hydrolysis rate and the final glucose yield. The highest glucose yield was obtained at 40°C. The yield decreased with increasing temperature, due to increased enzyme deactivation at higher temperatures, while the initial reaction rate increased with increasing temperatures.}},
author = {{Eklund, R and Galbe, Mats and Zacchi, Guido}},
issn = {{0141-0229}},
language = {{eng}},
number = {{3}},
pages = {{225--228}},
publisher = {{Elsevier}},
series = {{Enzyme and Microbial Technology}},
title = {{Optimization of temperature and enzyme concentration in the enzymatic saccharification of steam-pretreated willow}},
url = {{http://dx.doi.org/10.1016/0141-0229(90)90043-P}},
doi = {{10.1016/0141-0229(90)90043-P}},
volume = {{12}},
year = {{1990}},
}