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Optimization of temperature and enzyme concentration in the enzymatic saccharification of steam-pretreated willow

Eklund, R; Galbe, Mats LU and Zacchi, Guido LU (1990) In Enzyme and Microbial Technology 12(3). p.225-228
Abstract
The effect of temperature and enzyme concentration on the enzymatic hydrolysis of steam-pretreated willow was investigated to obtain the optimal hydrolysis conditions. The willow was pretreated at 220°C for 10 min according to the results of earlier optimization studies of the steam-pretreatment conditions for high glucose yields. The concentrations of two commercial enzymes, Celluclast 2L and Novozyme 188, were varied in the range 0–20% (based on oven-dry substrate). A maximum in the conversion of cellulose to glucose was obtained using a mixture of 15% Celluclast and 3% Novozyme, corresponding to an activity of 11.4 FPU g−1 substrate. Higher enzyme concentrations did not increase the initial hydrolysis rate nor affect the final glucose... (More)
The effect of temperature and enzyme concentration on the enzymatic hydrolysis of steam-pretreated willow was investigated to obtain the optimal hydrolysis conditions. The willow was pretreated at 220°C for 10 min according to the results of earlier optimization studies of the steam-pretreatment conditions for high glucose yields. The concentrations of two commercial enzymes, Celluclast 2L and Novozyme 188, were varied in the range 0–20% (based on oven-dry substrate). A maximum in the conversion of cellulose to glucose was obtained using a mixture of 15% Celluclast and 3% Novozyme, corresponding to an activity of 11.4 FPU g−1 substrate. Higher enzyme concentrations did not increase the initial hydrolysis rate nor affect the final glucose yield. The effects of temperature in the range 40°C to 60°C were investigated. The temperature affects both the initial hydrolysis rate and the final glucose yield. The highest glucose yield was obtained at 40°C. The yield decreased with increasing temperature, due to increased enzyme deactivation at higher temperatures, while the initial reaction rate increased with increasing temperatures. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Enzyme and Microbial Technology
volume
12
issue
3
pages
225 - 228
publisher
Elsevier
external identifiers
  • wos:A1990CN67700011
  • scopus:0025400283
ISSN
0141-0229
DOI
10.1016/0141-0229(90)90043-P
language
English
LU publication?
yes
id
f06f314e-f144-4c7d-92c0-43ca4c548fe1 (old id 3911615)
date added to LUP
2013-06-28 10:56:50
date last changed
2017-09-10 03:41:38
@article{f06f314e-f144-4c7d-92c0-43ca4c548fe1,
  abstract     = {The effect of temperature and enzyme concentration on the enzymatic hydrolysis of steam-pretreated willow was investigated to obtain the optimal hydrolysis conditions. The willow was pretreated at 220°C for 10 min according to the results of earlier optimization studies of the steam-pretreatment conditions for high glucose yields. The concentrations of two commercial enzymes, Celluclast 2L and Novozyme 188, were varied in the range 0–20% (based on oven-dry substrate). A maximum in the conversion of cellulose to glucose was obtained using a mixture of 15% Celluclast and 3% Novozyme, corresponding to an activity of 11.4 FPU g−1 substrate. Higher enzyme concentrations did not increase the initial hydrolysis rate nor affect the final glucose yield. The effects of temperature in the range 40°C to 60°C were investigated. The temperature affects both the initial hydrolysis rate and the final glucose yield. The highest glucose yield was obtained at 40°C. The yield decreased with increasing temperature, due to increased enzyme deactivation at higher temperatures, while the initial reaction rate increased with increasing temperatures.},
  author       = {Eklund, R and Galbe, Mats and Zacchi, Guido},
  issn         = {0141-0229},
  language     = {eng},
  number       = {3},
  pages        = {225--228},
  publisher    = {Elsevier},
  series       = {Enzyme and Microbial Technology},
  title        = {Optimization of temperature and enzyme concentration in the enzymatic saccharification of steam-pretreated willow},
  url          = {http://dx.doi.org/10.1016/0141-0229(90)90043-P},
  volume       = {12},
  year         = {1990},
}