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Investigating the kinetics of the enzymatic depolymerization of polygalacturonic acid in continuous UF-Membrane reactors

Gallifuoco, Alberto; Cantarella, Maria and Marucci, Mariagrazia LU (2007) In Biotechnology Progress 23(5). p.1111-1117
Abstract
A stirred tank membrane reactor is used to study the kinetics of polygalacturonic acid (PGA) enzymatic hydrolysis. The reactor operates in semicontinuous configuration: the native biopolymer is loaded at the initial time and the system is continuously fed with the buffer. The effect of retention time (from 10 1 to 142 min) and membrane molecular weight cutoff (from I to 30 kDa) on the rate of permeable oligomers production is investigated. Reaction products are clustered in two different classes, those sized below the membrane cutoff and those above. The reducing power measured in the permeate is used as an estimate of total product concentration. The characteristic breakdown times range from 40 to 100 min. The overall kinetics obeys a... (More)
A stirred tank membrane reactor is used to study the kinetics of polygalacturonic acid (PGA) enzymatic hydrolysis. The reactor operates in semicontinuous configuration: the native biopolymer is loaded at the initial time and the system is continuously fed with the buffer. The effect of retention time (from 10 1 to 142 min) and membrane molecular weight cutoff (from I to 30 kDa) on the rate of permeable oligomers production is investigated. Reaction products are clustered in two different classes, those sized below the membrane cutoff and those above. The reducing power measured in the permeate is used as an estimate of total product concentration. The characteristic breakdown times range from 40 to 100 min. The overall kinetics obeys a first-order law with a characteristic time estimated to 24 min. New mathematical data handling are developed and illustrated using the experimental data obtained. Finally, the body of the experimental results suggests useful indications (reactor productivity, breakdown induction period) for implementing the bioprocess at the industrial scale. (Less)
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author
publishing date
type
Contribution to journal
publication status
published
subject
in
Biotechnology Progress
volume
23
issue
5
pages
1111 - 1117
publisher
The American Chemical Society
external identifiers
  • wos:000250010000018
  • scopus:35348926825
ISSN
1520-6033
DOI
10.1021/bp070177y
language
English
LU publication?
no
id
36054928-0680-4841-a56a-f150dbe4113b (old id 3917196)
date added to LUP
2013-07-03 12:13:46
date last changed
2017-01-01 06:53:36
@article{36054928-0680-4841-a56a-f150dbe4113b,
  abstract     = {A stirred tank membrane reactor is used to study the kinetics of polygalacturonic acid (PGA) enzymatic hydrolysis. The reactor operates in semicontinuous configuration: the native biopolymer is loaded at the initial time and the system is continuously fed with the buffer. The effect of retention time (from 10 1 to 142 min) and membrane molecular weight cutoff (from I to 30 kDa) on the rate of permeable oligomers production is investigated. Reaction products are clustered in two different classes, those sized below the membrane cutoff and those above. The reducing power measured in the permeate is used as an estimate of total product concentration. The characteristic breakdown times range from 40 to 100 min. The overall kinetics obeys a first-order law with a characteristic time estimated to 24 min. New mathematical data handling are developed and illustrated using the experimental data obtained. Finally, the body of the experimental results suggests useful indications (reactor productivity, breakdown induction period) for implementing the bioprocess at the industrial scale.},
  author       = {Gallifuoco, Alberto and Cantarella, Maria and Marucci, Mariagrazia},
  issn         = {1520-6033},
  language     = {eng},
  number       = {5},
  pages        = {1111--1117},
  publisher    = {The American Chemical Society},
  series       = {Biotechnology Progress},
  title        = {Investigating the kinetics of the enzymatic depolymerization of polygalacturonic acid in continuous UF-Membrane reactors},
  url          = {http://dx.doi.org/10.1021/bp070177y},
  volume       = {23},
  year         = {2007},
}