Polygalacturonic acid/endo-polygalacturonase system: A kinetic study in batch reactors
(2004) In Biotechnology Progress 20(5). p.1430-1436- Abstract
- The enzymatic depolymerization of the pectic substance polygalacturonic acid (PGA) is studied in batch reactor. The number-average molecular weight of native substrate is estimated, using a simple and quick technique, to be approximately 11.1 kDa, the polymeric chains consisting on average of 63 galacturonic acid units. The effect of enzyme concentration was studied varying biocatalyst loading from 6 to 242 mg/L. The experiments were repeated at substrate concentrations ranging from 0.5 to 5 g/L. Data obtained at both short reaction time (20 min) and prolonged enzyme action (up to 350 min) are correlated using different kinetic equations, and the parameter values are discussed.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3917204
- author
- Gallifuoco, A ; Cantarella, M ; Viparelli, P and Marucci, Mariagrazia LU
- publishing date
- 2004
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Biotechnology Progress
- volume
- 20
- issue
- 5
- pages
- 1430 - 1436
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- wos:000224377700017
- scopus:5444225371
- pmid:15458327
- ISSN
- 1520-6033
- DOI
- 10.1021/bp049853b
- language
- English
- LU publication?
- no
- id
- c7bcc291-0018-4b3e-804b-8fb84b1c7d4b (old id 3917204)
- date added to LUP
- 2016-04-01 15:23:22
- date last changed
- 2022-03-14 18:00:38
@article{c7bcc291-0018-4b3e-804b-8fb84b1c7d4b, abstract = {{The enzymatic depolymerization of the pectic substance polygalacturonic acid (PGA) is studied in batch reactor. The number-average molecular weight of native substrate is estimated, using a simple and quick technique, to be approximately 11.1 kDa, the polymeric chains consisting on average of 63 galacturonic acid units. The effect of enzyme concentration was studied varying biocatalyst loading from 6 to 242 mg/L. The experiments were repeated at substrate concentrations ranging from 0.5 to 5 g/L. Data obtained at both short reaction time (20 min) and prolonged enzyme action (up to 350 min) are correlated using different kinetic equations, and the parameter values are discussed.}}, author = {{Gallifuoco, A and Cantarella, M and Viparelli, P and Marucci, Mariagrazia}}, issn = {{1520-6033}}, language = {{eng}}, number = {{5}}, pages = {{1430--1436}}, publisher = {{The American Chemical Society (ACS)}}, series = {{Biotechnology Progress}}, title = {{Polygalacturonic acid/endo-polygalacturonase system: A kinetic study in batch reactors}}, url = {{http://dx.doi.org/10.1021/bp049853b}}, doi = {{10.1021/bp049853b}}, volume = {{20}}, year = {{2004}}, }