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Polygalacturonic acid/endo-polygalacturonase system: A kinetic study in batch reactors

Gallifuoco, A; Cantarella, M; Viparelli, P and Marucci, Mariagrazia LU (2004) In Biotechnology Progress 20(5). p.1430-1436
Abstract
The enzymatic depolymerization of the pectic substance polygalacturonic acid (PGA) is studied in batch reactor. The number-average molecular weight of native substrate is estimated, using a simple and quick technique, to be approximately 11.1 kDa, the polymeric chains consisting on average of 63 galacturonic acid units. The effect of enzyme concentration was studied varying biocatalyst loading from 6 to 242 mg/L. The experiments were repeated at substrate concentrations ranging from 0.5 to 5 g/L. Data obtained at both short reaction time (20 min) and prolonged enzyme action (up to 350 min) are correlated using different kinetic equations, and the parameter values are discussed.
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author
publishing date
type
Contribution to journal
publication status
published
subject
in
Biotechnology Progress
volume
20
issue
5
pages
1430 - 1436
publisher
The American Chemical Society
external identifiers
  • wos:000224377700017
  • scopus:5444225371
ISSN
1520-6033
DOI
10.1021/bp049853b
language
English
LU publication?
no
id
c7bcc291-0018-4b3e-804b-8fb84b1c7d4b (old id 3917204)
date added to LUP
2013-07-03 12:18:29
date last changed
2017-01-01 06:38:44
@article{c7bcc291-0018-4b3e-804b-8fb84b1c7d4b,
  abstract     = {The enzymatic depolymerization of the pectic substance polygalacturonic acid (PGA) is studied in batch reactor. The number-average molecular weight of native substrate is estimated, using a simple and quick technique, to be approximately 11.1 kDa, the polymeric chains consisting on average of 63 galacturonic acid units. The effect of enzyme concentration was studied varying biocatalyst loading from 6 to 242 mg/L. The experiments were repeated at substrate concentrations ranging from 0.5 to 5 g/L. Data obtained at both short reaction time (20 min) and prolonged enzyme action (up to 350 min) are correlated using different kinetic equations, and the parameter values are discussed.},
  author       = {Gallifuoco, A and Cantarella, M and Viparelli, P and Marucci, Mariagrazia},
  issn         = {1520-6033},
  language     = {eng},
  number       = {5},
  pages        = {1430--1436},
  publisher    = {The American Chemical Society},
  series       = {Biotechnology Progress},
  title        = {Polygalacturonic acid/endo-polygalacturonase system: A kinetic study in batch reactors},
  url          = {http://dx.doi.org/10.1021/bp049853b},
  volume       = {20},
  year         = {2004},
}