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Direct measurement of intraparticle fluid velocity in superporous agarose beads

Larsson, Per-Olof LU ; Gustavsson, Per-Erik LU and Axelsson, Anders LU (1998) In Journal of Molecular Recognition 11(1-6). p.270-272
Abstract
Superporous agarose beads contain both normal diffusion pores and special, very wide superpores through which part of the chromatographic flow is transported, a situation that may greatly improve the chromatographic performance. For the first time such pore flow was measured directly by following the movement of microparticles (dyed yeast cells) through superporous beads packed in a chromatographic bed. The passage of the microparticles through the superpores and through the interstitial pores was recorded by a microscope/video camera, The video recordings were subsequently used to determine flow paths as well as the convective fluid velocities in both the superpores and the interstitial pores. The superpore fluid velocity was found to be... (More)
Superporous agarose beads contain both normal diffusion pores and special, very wide superpores through which part of the chromatographic flow is transported, a situation that may greatly improve the chromatographic performance. For the first time such pore flow was measured directly by following the movement of microparticles (dyed yeast cells) through superporous beads packed in a chromatographic bed. The passage of the microparticles through the superpores and through the interstitial pores was recorded by a microscope/video camera, The video recordings were subsequently used to determine flow paths as well as the convective fluid velocities in both the superpores and the interstitial pores. The superpore fluid velocity was found to be proportional to the ratio between the squares of the respective pore diameters, which is in agreement with the Kozeny-Carman equation, Values for two-dimensional and three-dimensional tortuosity of the flow paths were measured and calculated respectively. (C) 1998 John Wiley & Sons, Ltd. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
superporous agarose, superpore, pore flow, intraparticle convection
in
Journal of Molecular Recognition
volume
11
issue
1-6
pages
270 - 272
publisher
John Wiley & Sons
external identifiers
  • wos:000078789000061
  • scopus:0032457548
ISSN
1099-1352
DOI
10.1002/(SICI)1099-1352(199812)11:1/6<270::AID-JMR439>3.0.CO;2-I
language
English
LU publication?
yes
id
3907ec82-04fc-4a12-9e04-cb968bb1254b (old id 3917239)
date added to LUP
2013-07-03 12:41:59
date last changed
2017-01-01 04:44:21
@article{3907ec82-04fc-4a12-9e04-cb968bb1254b,
  abstract     = {Superporous agarose beads contain both normal diffusion pores and special, very wide superpores through which part of the chromatographic flow is transported, a situation that may greatly improve the chromatographic performance. For the first time such pore flow was measured directly by following the movement of microparticles (dyed yeast cells) through superporous beads packed in a chromatographic bed. The passage of the microparticles through the superpores and through the interstitial pores was recorded by a microscope/video camera, The video recordings were subsequently used to determine flow paths as well as the convective fluid velocities in both the superpores and the interstitial pores. The superpore fluid velocity was found to be proportional to the ratio between the squares of the respective pore diameters, which is in agreement with the Kozeny-Carman equation, Values for two-dimensional and three-dimensional tortuosity of the flow paths were measured and calculated respectively. (C) 1998 John Wiley &amp; Sons, Ltd.},
  author       = {Larsson, Per-Olof and Gustavsson, Per-Erik and Axelsson, Anders},
  issn         = {1099-1352},
  keyword      = {superporous agarose,superpore,pore flow,intraparticle convection},
  language     = {eng},
  number       = {1-6},
  pages        = {270--272},
  publisher    = {John Wiley & Sons},
  series       = {Journal of Molecular Recognition},
  title        = {Direct measurement of intraparticle fluid velocity in superporous agarose beads},
  url          = {http://dx.doi.org/10.1002/(SICI)1099-1352(199812)11:1/6<270::AID-JMR439>3.0.CO;2-I},
  volume       = {11},
  year         = {1998},
}