Use of dynamic step response for control of fed-batch conversion of lignocellulosic hydrolyzates to ethanol
(2001) In Journal of Biotechnology 89(1). p.41-53- Abstract
- Optimization of fed-batch conversion of lignocellulosic hydrolyzates by the yeast Saccharomyces cerevisiae was studied. The feed rate was controlled using a step response strategy, in which the carbon dioxide evolution rate was used as input variable. The performance of the control strategy was examined using both an untreated and a detoxified dilute acid hydrolyzate, and the performance was compared to that obtained with a synthetic medium. In batch cultivation of the untreated hydrolyzate, only 23% of the hexose sugars were assimilated. However, by using the feed-back controlled fed-batch technique, it was possible to obtain complete conversion of the hexose sugars. Furthermore, the maximal specific ethanol productivity (q(t.max))... (More)
- Optimization of fed-batch conversion of lignocellulosic hydrolyzates by the yeast Saccharomyces cerevisiae was studied. The feed rate was controlled using a step response strategy, in which the carbon dioxide evolution rate was used as input variable. The performance of the control strategy was examined using both an untreated and a detoxified dilute acid hydrolyzate, and the performance was compared to that obtained with a synthetic medium. In batch cultivation of the untreated hydrolyzate, only 23% of the hexose sugars were assimilated. However, by using the feed-back controlled fed-batch technique, it was possible to obtain complete conversion of the hexose sugars. Furthermore, the maximal specific ethanol productivity (q(t.max)) increased more than 10-fold, from 0.06 to 0.70 g g(-1) h(-1). In addition, the viability of the yeast cells decreased by more than 99% in batch cultivation, whereas a viability of more than 40% could be maintained during fed-batch cultivation. In contrast to untreated hydrolyzate, it was possible to convert the sugars in the detoxified hydrolyzate also in batch cultivation. However, a 50% higher specific ethanol productivity was obtained using fed-batch cultivation. During batch cultivation of both untreated and detoxified hydrolyzate a gradual decrease in specific ethanol productivity was observed. This decrease could largely be avoided in fed-batch cultivations. (C) 2001 Elsevier Science B.V. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3917759
- author
- Nilsson, Anneli LU ; Taherzadeh, MJ and Lidén, Gunnar LU
- organization
- publishing date
- 2001
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- ethanol, Saccharomyces cerevisiae, dilute acid hydrolyzate, inhibitors, control, fed-batch
- in
- Journal of Biotechnology
- volume
- 89
- issue
- 1
- pages
- 41 - 53
- publisher
- Elsevier
- external identifiers
-
- wos:000170319800004
- scopus:0035954535
- ISSN
- 1873-4863
- DOI
- 10.1016/S0168-1656(01)00283-8
- language
- English
- LU publication?
- yes
- id
- f38add50-2057-4fa8-9768-d5c008cddac2 (old id 3917759)
- date added to LUP
- 2016-04-01 11:36:17
- date last changed
- 2023-11-10 17:52:35
@article{f38add50-2057-4fa8-9768-d5c008cddac2,
abstract = {{Optimization of fed-batch conversion of lignocellulosic hydrolyzates by the yeast Saccharomyces cerevisiae was studied. The feed rate was controlled using a step response strategy, in which the carbon dioxide evolution rate was used as input variable. The performance of the control strategy was examined using both an untreated and a detoxified dilute acid hydrolyzate, and the performance was compared to that obtained with a synthetic medium. In batch cultivation of the untreated hydrolyzate, only 23% of the hexose sugars were assimilated. However, by using the feed-back controlled fed-batch technique, it was possible to obtain complete conversion of the hexose sugars. Furthermore, the maximal specific ethanol productivity (q(t.max)) increased more than 10-fold, from 0.06 to 0.70 g g(-1) h(-1). In addition, the viability of the yeast cells decreased by more than 99% in batch cultivation, whereas a viability of more than 40% could be maintained during fed-batch cultivation. In contrast to untreated hydrolyzate, it was possible to convert the sugars in the detoxified hydrolyzate also in batch cultivation. However, a 50% higher specific ethanol productivity was obtained using fed-batch cultivation. During batch cultivation of both untreated and detoxified hydrolyzate a gradual decrease in specific ethanol productivity was observed. This decrease could largely be avoided in fed-batch cultivations. (C) 2001 Elsevier Science B.V. All rights reserved.}},
author = {{Nilsson, Anneli and Taherzadeh, MJ and Lidén, Gunnar}},
issn = {{1873-4863}},
keywords = {{ethanol; Saccharomyces cerevisiae; dilute acid hydrolyzate; inhibitors; control; fed-batch}},
language = {{eng}},
number = {{1}},
pages = {{41--53}},
publisher = {{Elsevier}},
series = {{Journal of Biotechnology}},
title = {{Use of dynamic step response for control of fed-batch conversion of lignocellulosic hydrolyzates to ethanol}},
url = {{http://dx.doi.org/10.1016/S0168-1656(01)00283-8}},
doi = {{10.1016/S0168-1656(01)00283-8}},
volume = {{89}},
year = {{2001}},
}