Advanced

Use of dynamic step response for control of fed-batch conversion of lignocellulosic hydrolyzates to ethanol

Nilsson, Anneli LU ; Taherzadeh, MJ and Lidén, Gunnar LU (2001) In Journal of Biotechnology 89(1). p.41-53
Abstract
Optimization of fed-batch conversion of lignocellulosic hydrolyzates by the yeast Saccharomyces cerevisiae was studied. The feed rate was controlled using a step response strategy, in which the carbon dioxide evolution rate was used as input variable. The performance of the control strategy was examined using both an untreated and a detoxified dilute acid hydrolyzate, and the performance was compared to that obtained with a synthetic medium. In batch cultivation of the untreated hydrolyzate, only 23% of the hexose sugars were assimilated. However, by using the feed-back controlled fed-batch technique, it was possible to obtain complete conversion of the hexose sugars. Furthermore, the maximal specific ethanol productivity (q(t.max))... (More)
Optimization of fed-batch conversion of lignocellulosic hydrolyzates by the yeast Saccharomyces cerevisiae was studied. The feed rate was controlled using a step response strategy, in which the carbon dioxide evolution rate was used as input variable. The performance of the control strategy was examined using both an untreated and a detoxified dilute acid hydrolyzate, and the performance was compared to that obtained with a synthetic medium. In batch cultivation of the untreated hydrolyzate, only 23% of the hexose sugars were assimilated. However, by using the feed-back controlled fed-batch technique, it was possible to obtain complete conversion of the hexose sugars. Furthermore, the maximal specific ethanol productivity (q(t.max)) increased more than 10-fold, from 0.06 to 0.70 g g(-1) h(-1). In addition, the viability of the yeast cells decreased by more than 99% in batch cultivation, whereas a viability of more than 40% could be maintained during fed-batch cultivation. In contrast to untreated hydrolyzate, it was possible to convert the sugars in the detoxified hydrolyzate also in batch cultivation. However, a 50% higher specific ethanol productivity was obtained using fed-batch cultivation. During batch cultivation of both untreated and detoxified hydrolyzate a gradual decrease in specific ethanol productivity was observed. This decrease could largely be avoided in fed-batch cultivations. (C) 2001 Elsevier Science B.V. All rights reserved. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
ethanol, Saccharomyces cerevisiae, dilute acid hydrolyzate, inhibitors, control, fed-batch
in
Journal of Biotechnology
volume
89
issue
1
pages
41 - 53
publisher
Elsevier
external identifiers
  • wos:000170319800004
  • scopus:0035954535
ISSN
1873-4863
DOI
10.1016/S0168-1656(01)00283-8
language
English
LU publication?
yes
id
f38add50-2057-4fa8-9768-d5c008cddac2 (old id 3917759)
date added to LUP
2013-07-04 11:19:16
date last changed
2018-05-29 09:34:03
@article{f38add50-2057-4fa8-9768-d5c008cddac2,
  abstract     = {Optimization of fed-batch conversion of lignocellulosic hydrolyzates by the yeast Saccharomyces cerevisiae was studied. The feed rate was controlled using a step response strategy, in which the carbon dioxide evolution rate was used as input variable. The performance of the control strategy was examined using both an untreated and a detoxified dilute acid hydrolyzate, and the performance was compared to that obtained with a synthetic medium. In batch cultivation of the untreated hydrolyzate, only 23% of the hexose sugars were assimilated. However, by using the feed-back controlled fed-batch technique, it was possible to obtain complete conversion of the hexose sugars. Furthermore, the maximal specific ethanol productivity (q(t.max)) increased more than 10-fold, from 0.06 to 0.70 g g(-1) h(-1). In addition, the viability of the yeast cells decreased by more than 99% in batch cultivation, whereas a viability of more than 40% could be maintained during fed-batch cultivation. In contrast to untreated hydrolyzate, it was possible to convert the sugars in the detoxified hydrolyzate also in batch cultivation. However, a 50% higher specific ethanol productivity was obtained using fed-batch cultivation. During batch cultivation of both untreated and detoxified hydrolyzate a gradual decrease in specific ethanol productivity was observed. This decrease could largely be avoided in fed-batch cultivations. (C) 2001 Elsevier Science B.V. All rights reserved.},
  author       = {Nilsson, Anneli and Taherzadeh, MJ and Lidén, Gunnar},
  issn         = {1873-4863},
  keyword      = {ethanol,Saccharomyces cerevisiae,dilute acid hydrolyzate,inhibitors,control,fed-batch},
  language     = {eng},
  number       = {1},
  pages        = {41--53},
  publisher    = {Elsevier},
  series       = {Journal of Biotechnology},
  title        = {Use of dynamic step response for control of fed-batch conversion of lignocellulosic hydrolyzates to ethanol},
  url          = {http://dx.doi.org/10.1016/S0168-1656(01)00283-8},
  volume       = {89},
  year         = {2001},
}