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Identification of protein expression signatures associated with Helicobacter pylori infection and gastric adenocarcinoma using recombinant antibody microarrays

Ellmark, Peter LU ; Ingvarsson, Johan LU ; Carlsson, Anders LU ; Lundin, B. Samuel ; Wingren, Christer LU and Borrebaeck, Carl LU (2006) In Molecular & Cellular Proteomics 5(9). p.1638-1646
Abstract
Antibody microarray based technology is a powerful emerging tool in proteomics, target discovery, and differential analysis. Here, we report the first study where recombinant antibody fragments have been used to construct large scale antibody microarrays, composed of 127 different antibodies against mostly immunoregulatory antigens. The arrays were based on single framework recombinant antibody fragments ( SinFabs) designed for high on-chip stability and functionality and were used for the analysis of malignant and normal stomach tissue samples from Helicobacter pylori-positive and - negative patients. Our results demonstrate that distinct tumor- as well as infection-associated protein expression signatures could be identified from these... (More)
Antibody microarray based technology is a powerful emerging tool in proteomics, target discovery, and differential analysis. Here, we report the first study where recombinant antibody fragments have been used to construct large scale antibody microarrays, composed of 127 different antibodies against mostly immunoregulatory antigens. The arrays were based on single framework recombinant antibody fragments ( SinFabs) designed for high on-chip stability and functionality and were used for the analysis of malignant and normal stomach tissue samples from Helicobacter pylori-positive and - negative patients. Our results demonstrate that distinct tumor- as well as infection-associated protein expression signatures could be identified from these complex tissue proteomes, as well as biomarkers such as IL-9, IL-11, and MCP-4, previously not found in these diseases. In a longer perspective, this study may improve the understanding of H. pylori-induced stomach cancer and lead to development of improved diagnostics. (Less)
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author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Molecular & Cellular Proteomics
volume
5
issue
9
pages
1638 - 1646
publisher
American Society for Biochemistry and Molecular Biology
external identifiers
  • wos:000240551800008
  • pmid:16844680
  • scopus:33749243597
ISSN
1535-9484
DOI
10.1074/mcp.M600170-MCP200
language
English
LU publication?
yes
id
9535b78f-ec83-4f94-b4d6-d9d0a1311d93 (old id 392985)
date added to LUP
2016-04-01 12:08:42
date last changed
2021-09-22 05:09:13
@article{9535b78f-ec83-4f94-b4d6-d9d0a1311d93,
  abstract     = {Antibody microarray based technology is a powerful emerging tool in proteomics, target discovery, and differential analysis. Here, we report the first study where recombinant antibody fragments have been used to construct large scale antibody microarrays, composed of 127 different antibodies against mostly immunoregulatory antigens. The arrays were based on single framework recombinant antibody fragments ( SinFabs) designed for high on-chip stability and functionality and were used for the analysis of malignant and normal stomach tissue samples from Helicobacter pylori-positive and - negative patients. Our results demonstrate that distinct tumor- as well as infection-associated protein expression signatures could be identified from these complex tissue proteomes, as well as biomarkers such as IL-9, IL-11, and MCP-4, previously not found in these diseases. In a longer perspective, this study may improve the understanding of H. pylori-induced stomach cancer and lead to development of improved diagnostics.},
  author       = {Ellmark, Peter and Ingvarsson, Johan and Carlsson, Anders and Lundin, B. Samuel and Wingren, Christer and Borrebaeck, Carl},
  issn         = {1535-9484},
  language     = {eng},
  number       = {9},
  pages        = {1638--1646},
  publisher    = {American Society for Biochemistry and Molecular Biology},
  series       = {Molecular & Cellular Proteomics},
  title        = {Identification of protein expression signatures associated with Helicobacter pylori infection and gastric adenocarcinoma using recombinant antibody microarrays},
  url          = {http://dx.doi.org/10.1074/mcp.M600170-MCP200},
  doi          = {10.1074/mcp.M600170-MCP200},
  volume       = {5},
  year         = {2006},
}