Evaluation of polytyramine film and 6-mercaptohexanol self-assembled monolayers as the immobilization layers for a capacitive dna sensor chip : A comparison
(2021) In Sensors 21(23).- Abstract
The performance of a biosensor is associated with the properties of an immobilization layer on a sensor chip. In this study, gold sensor chips were modified with two different immobilization layers, polytyramine film and 6-mercaptohexanol self-assembled monolayer. The physical, electrochemical and analytical properties of polytyramine film and mercaptohexanol self-assembled monolayer modified gold sensor chips were studied and compared. The study was conducted using atomic force microscopy, cyclic voltammetry and a capacitive DNA-sensor system (CapSenze™ Biosystem). The results obtained by atomic force microscopy and cyclic voltammetry indicate that polytyramine film on the sensor chip surface possesses better insulating properties and... (More)
The performance of a biosensor is associated with the properties of an immobilization layer on a sensor chip. In this study, gold sensor chips were modified with two different immobilization layers, polytyramine film and 6-mercaptohexanol self-assembled monolayer. The physical, electrochemical and analytical properties of polytyramine film and mercaptohexanol self-assembled monolayer modified gold sensor chips were studied and compared. The study was conducted using atomic force microscopy, cyclic voltammetry and a capacitive DNA-sensor system (CapSenze™ Biosystem). The results obtained by atomic force microscopy and cyclic voltammetry indicate that polytyramine film on the sensor chip surface possesses better insulating properties and provides more spaces for the immobilization of the capture probe than a mercaptohexanol self-assembled monolayer. A capacitive DNA sensor hosting a polytyramine single-stranded DNA-modified sensor chip displayed higher sensitivity and larger signal amplitude than that of a mercaptohexanol singlestranded DNA-modified sensor chip. The linearity responses for polytyramine single-stranded DNAand mercaptohexanol single-stranded DNA-modified sensor chips were obtained at log concentration ranges, equivalent to 10−12 to 10−8 M and 10−10 to 10−8 M, with detection limits of 4.0 × 10−13 M and 7.0 × 10−11 M of target complementary single-stranded DNA, respectively. Mercaptohexanol single-stranded DNA-and polytyramine single-stranded DNA-modified sensor chips exhibited a notable selectivity at an elevated hybridization temperature of 50◦C, albeit the signal amplitudes due to the hybridization of the target complementary single-stranded DNA were reduced by almost 20% and less than 5%, respectively.
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- author
- Mahadhy, Ally ; Mattiasson, Bo LU ; Ståhlwernersson, Eva LU and Hedström, Martin LU
- organization
- publishing date
- 2021-12-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- 6-mercaptohexanol, Biosensor matrix, Electropolymerization, Polytyramine, Self-assembled monolayer, Sensor chip
- in
- Sensors
- volume
- 21
- issue
- 23
- article number
- 8149
- publisher
- MDPI AG
- external identifiers
-
- pmid:34884153
- scopus:85120634510
- ISSN
- 1424-8220
- DOI
- 10.3390/s21238149
- language
- English
- LU publication?
- yes
- additional info
- Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
- id
- 39335ca4-0fbe-4401-9c9a-9c7628baf5b5
- date added to LUP
- 2022-01-25 13:55:11
- date last changed
- 2024-06-16 00:23:13
@article{39335ca4-0fbe-4401-9c9a-9c7628baf5b5,
abstract = {{<p>The performance of a biosensor is associated with the properties of an immobilization layer on a sensor chip. In this study, gold sensor chips were modified with two different immobilization layers, polytyramine film and 6-mercaptohexanol self-assembled monolayer. The physical, electrochemical and analytical properties of polytyramine film and mercaptohexanol self-assembled monolayer modified gold sensor chips were studied and compared. The study was conducted using atomic force microscopy, cyclic voltammetry and a capacitive DNA-sensor system (CapSenze™ Biosystem). The results obtained by atomic force microscopy and cyclic voltammetry indicate that polytyramine film on the sensor chip surface possesses better insulating properties and provides more spaces for the immobilization of the capture probe than a mercaptohexanol self-assembled monolayer. A capacitive DNA sensor hosting a polytyramine single-stranded DNA-modified sensor chip displayed higher sensitivity and larger signal amplitude than that of a mercaptohexanol singlestranded DNA-modified sensor chip. The linearity responses for polytyramine single-stranded DNAand mercaptohexanol single-stranded DNA-modified sensor chips were obtained at log concentration ranges, equivalent to 10<sup>−12</sup> to 10<sup>−8</sup> M and 10<sup>−10</sup> to 10<sup>−8</sup> M, with detection limits of 4.0 × 10<sup>−13</sup> M and 7.0 × 10<sup>−11</sup> M of target complementary single-stranded DNA, respectively. Mercaptohexanol single-stranded DNA-and polytyramine single-stranded DNA-modified sensor chips exhibited a notable selectivity at an elevated hybridization temperature of 50<sup>◦</sup>C, albeit the signal amplitudes due to the hybridization of the target complementary single-stranded DNA were reduced by almost 20% and less than 5%, respectively.</p>}},
author = {{Mahadhy, Ally and Mattiasson, Bo and Ståhlwernersson, Eva and Hedström, Martin}},
issn = {{1424-8220}},
keywords = {{6-mercaptohexanol; Biosensor matrix; Electropolymerization; Polytyramine; Self-assembled monolayer; Sensor chip}},
language = {{eng}},
month = {{12}},
number = {{23}},
publisher = {{MDPI AG}},
series = {{Sensors}},
title = {{Evaluation of polytyramine film and 6-mercaptohexanol self-assembled monolayers as the immobilization layers for a capacitive dna sensor chip : A comparison}},
url = {{http://dx.doi.org/10.3390/s21238149}},
doi = {{10.3390/s21238149}},
volume = {{21}},
year = {{2021}},
}