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Antibodies of IgM subclass to phosphorylcholine and oxidized LDL are protective factors for atherosclerosis in patients with hypertension

Su, Jun ; Georgiades, Anastasia ; Wu, Ruihua ; Thulin, Thomas LU ; de Faire, Ulf and Frostegard, Johan (2006) In Atherosclerosis 188(1). p.160-166
Abstract
Objective: To determine the importance of antibodies against phosphorylcholine (PC) and oxidized low density lipoprotein (OxLDL) for development of atherosclerosis. Methods and results: Two hundred and twenty six individuals with established hypertension (diastolic pressure > 95 mmHg) were from European Lacidipine Study on Atherosclerosis. Antibodies of IgG and IgM subclass were tested by ELISA against PC (aPC), cupper-oxidized (ox)- or malondialdehyde (MDA)-modified LDL. High-sensitivity C-reactive protein was measured by nephelometry. As a surrogate measure of atherosclerosis, we used the mean of the maximum intima-media thicknesses (IMT) in the far walls of common carotids and bifurcations was determined by ultrasonography at the... (More)
Objective: To determine the importance of antibodies against phosphorylcholine (PC) and oxidized low density lipoprotein (OxLDL) for development of atherosclerosis. Methods and results: Two hundred and twenty six individuals with established hypertension (diastolic pressure > 95 mmHg) were from European Lacidipine Study on Atherosclerosis. Antibodies of IgG and IgM subclass were tested by ELISA against PC (aPC), cupper-oxidized (ox)- or malondialdehyde (MDA)-modified LDL. High-sensitivity C-reactive protein was measured by nephelometry. As a surrogate measure of atherosclerosis, we used the mean of the maximum intima-media thicknesses (IMT) in the far walls of common carotids and bifurcations was determined by ultrasonography at the time of enrolment, and 4 years following enrolment. aPC could be competed out by PC and OxLDL, while cardiolipin (CL) and beta 2-glycoprotein I (beta 2GPI) were less effective and phosphatidylserine (PS) not at all. Increases in IMT at follow-up were less common in subjects which at the time of enrolment had high IgM aPC (both 75th and 90th; odds ratios: 0.46; Cl: 0.25-0.85; 0.36; Cl: 0.15-0.87) and high IgM aOxLDL and aMDA-LDL (90th; odds ratios 0.27; p = 0.01; Cl: 0.11-0.69 and 0.27; p = 0.01; Cl: 0.11-0.69). CRP was unrelated to IMT-changes. The relationship between IgM aPC, aOxLDL and aMDA-LDL and changes in IMT was independent of age, treatment with atenolol or lacidipine, smoking and lipids. Women had higher levels of IgM antibodies tested (p < 0.05). Conclusions: High levels of IgM-antibodies against PC and OxLDL predict a favourable outcome in the development of carotid atherosclerosis in hypertensive subjects. Whether these antibodies could be used therapeutically deserves further study. (c) 2005 Elsevier Ireland Ltd. All rights reserved. (Less)
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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
oxidized, phosphorylcholine, antibodies, hypertension, atherosclerosis, low density lipoprotein
in
Atherosclerosis
volume
188
issue
1
pages
160 - 166
publisher
Elsevier
external identifiers
  • wos:000240178200021
  • scopus:33746773033
ISSN
1879-1484
DOI
10.1016/j.atherosclerosis.2005.10.017
language
English
LU publication?
yes
id
1f24f9b9-5d1d-40f9-9052-1f38401bc0ce (old id 394869)
date added to LUP
2016-04-01 11:40:51
date last changed
2021-10-06 03:47:13
@article{1f24f9b9-5d1d-40f9-9052-1f38401bc0ce,
  abstract     = {Objective: To determine the importance of antibodies against phosphorylcholine (PC) and oxidized low density lipoprotein (OxLDL) for development of atherosclerosis. Methods and results: Two hundred and twenty six individuals with established hypertension (diastolic pressure &gt; 95 mmHg) were from European Lacidipine Study on Atherosclerosis. Antibodies of IgG and IgM subclass were tested by ELISA against PC (aPC), cupper-oxidized (ox)- or malondialdehyde (MDA)-modified LDL. High-sensitivity C-reactive protein was measured by nephelometry. As a surrogate measure of atherosclerosis, we used the mean of the maximum intima-media thicknesses (IMT) in the far walls of common carotids and bifurcations was determined by ultrasonography at the time of enrolment, and 4 years following enrolment. aPC could be competed out by PC and OxLDL, while cardiolipin (CL) and beta 2-glycoprotein I (beta 2GPI) were less effective and phosphatidylserine (PS) not at all. Increases in IMT at follow-up were less common in subjects which at the time of enrolment had high IgM aPC (both 75th and 90th; odds ratios: 0.46; Cl: 0.25-0.85; 0.36; Cl: 0.15-0.87) and high IgM aOxLDL and aMDA-LDL (90th; odds ratios 0.27; p = 0.01; Cl: 0.11-0.69 and 0.27; p = 0.01; Cl: 0.11-0.69). CRP was unrelated to IMT-changes. The relationship between IgM aPC, aOxLDL and aMDA-LDL and changes in IMT was independent of age, treatment with atenolol or lacidipine, smoking and lipids. Women had higher levels of IgM antibodies tested (p &lt; 0.05). Conclusions: High levels of IgM-antibodies against PC and OxLDL predict a favourable outcome in the development of carotid atherosclerosis in hypertensive subjects. Whether these antibodies could be used therapeutically deserves further study. (c) 2005 Elsevier Ireland Ltd. All rights reserved.},
  author       = {Su, Jun and Georgiades, Anastasia and Wu, Ruihua and Thulin, Thomas and de Faire, Ulf and Frostegard, Johan},
  issn         = {1879-1484},
  language     = {eng},
  number       = {1},
  pages        = {160--166},
  publisher    = {Elsevier},
  series       = {Atherosclerosis},
  title        = {Antibodies of IgM subclass to phosphorylcholine and oxidized LDL are protective factors for atherosclerosis in patients with hypertension},
  url          = {http://dx.doi.org/10.1016/j.atherosclerosis.2005.10.017},
  doi          = {10.1016/j.atherosclerosis.2005.10.017},
  volume       = {188},
  year         = {2006},
}