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Conformational change of giant DNA with added salt as revealed by single molecular observation

Makita, Naoko ; Ullner, Magnus LU and Yoshikawa, Kenichi (2006) In Macromolecules 39(18). p.6200-6206
Abstract
The structural change of individual giant double-stranded T4 DNA (165.6 kilobase pairs) molecules as a function of salt concentration was investigated by single molecular observation with fluorescence microscopy in a wide range of concentration of NaCl, 1 x 10(-6)-3 M. The measured long-axis length was transformed into a persistence length and subjected to a power-law analysis, which showed that the electrostatic contribution to the persistence length is inversely proportional to the square root of the salt concentration; i.e., it has a linear dependence on the screening length. This is interpreted as the behavior of a flexible chain with electrostatic excluded-volume interactions. Although double-stranded DNA is locally stiff, it displays... (More)
The structural change of individual giant double-stranded T4 DNA (165.6 kilobase pairs) molecules as a function of salt concentration was investigated by single molecular observation with fluorescence microscopy in a wide range of concentration of NaCl, 1 x 10(-6)-3 M. The measured long-axis length was transformed into a persistence length and subjected to a power-law analysis, which showed that the electrostatic contribution to the persistence length is inversely proportional to the square root of the salt concentration; i.e., it has a linear dependence on the screening length. This is interpreted as the behavior of a flexible chain with electrostatic excluded-volume interactions. Although double-stranded DNA is locally stiff, it displays flexibility when the contour length is much longer than the persistence length, as is the case for T4 DNA. (Less)
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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Macromolecules
volume
39
issue
18
pages
6200 - 6206
publisher
The American Chemical Society (ACS)
external identifiers
  • wos:000240069300037
  • scopus:33748794230
ISSN
0024-9297
DOI
10.1021/ma060669b
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Theoretical Chemistry (S) (011001039)
id
801f0a38-bc1c-4a12-98f6-c04c1f2ef9c5 (old id 394997)
date added to LUP
2016-04-01 12:09:33
date last changed
2020-01-12 09:19:32
@article{801f0a38-bc1c-4a12-98f6-c04c1f2ef9c5,
  abstract     = {The structural change of individual giant double-stranded T4 DNA (165.6 kilobase pairs) molecules as a function of salt concentration was investigated by single molecular observation with fluorescence microscopy in a wide range of concentration of NaCl, 1 x 10(-6)-3 M. The measured long-axis length was transformed into a persistence length and subjected to a power-law analysis, which showed that the electrostatic contribution to the persistence length is inversely proportional to the square root of the salt concentration; i.e., it has a linear dependence on the screening length. This is interpreted as the behavior of a flexible chain with electrostatic excluded-volume interactions. Although double-stranded DNA is locally stiff, it displays flexibility when the contour length is much longer than the persistence length, as is the case for T4 DNA.},
  author       = {Makita, Naoko and Ullner, Magnus and Yoshikawa, Kenichi},
  issn         = {0024-9297},
  language     = {eng},
  number       = {18},
  pages        = {6200--6206},
  publisher    = {The American Chemical Society (ACS)},
  series       = {Macromolecules},
  title        = {Conformational change of giant DNA with added salt as revealed by single molecular observation},
  url          = {http://dx.doi.org/10.1021/ma060669b},
  doi          = {10.1021/ma060669b},
  volume       = {39},
  year         = {2006},
}