Conformational change of giant DNA with added salt as revealed by single molecular observation
(2006) In Macromolecules 39(18). p.6200-6206- Abstract
- The structural change of individual giant double-stranded T4 DNA (165.6 kilobase pairs) molecules as a function of salt concentration was investigated by single molecular observation with fluorescence microscopy in a wide range of concentration of NaCl, 1 x 10(-6)-3 M. The measured long-axis length was transformed into a persistence length and subjected to a power-law analysis, which showed that the electrostatic contribution to the persistence length is inversely proportional to the square root of the salt concentration; i.e., it has a linear dependence on the screening length. This is interpreted as the behavior of a flexible chain with electrostatic excluded-volume interactions. Although double-stranded DNA is locally stiff, it displays... (More)
- The structural change of individual giant double-stranded T4 DNA (165.6 kilobase pairs) molecules as a function of salt concentration was investigated by single molecular observation with fluorescence microscopy in a wide range of concentration of NaCl, 1 x 10(-6)-3 M. The measured long-axis length was transformed into a persistence length and subjected to a power-law analysis, which showed that the electrostatic contribution to the persistence length is inversely proportional to the square root of the salt concentration; i.e., it has a linear dependence on the screening length. This is interpreted as the behavior of a flexible chain with electrostatic excluded-volume interactions. Although double-stranded DNA is locally stiff, it displays flexibility when the contour length is much longer than the persistence length, as is the case for T4 DNA. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/394997
- author
- Makita, Naoko ; Ullner, Magnus LU and Yoshikawa, Kenichi
- organization
- publishing date
- 2006
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Macromolecules
- volume
- 39
- issue
- 18
- pages
- 6200 - 6206
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- wos:000240069300037
- scopus:33748794230
- ISSN
- 0024-9297
- DOI
- 10.1021/ma060669b
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Theoretical Chemistry (S) (011001039)
- id
- 801f0a38-bc1c-4a12-98f6-c04c1f2ef9c5 (old id 394997)
- date added to LUP
- 2016-04-01 12:09:33
- date last changed
- 2023-01-03 04:39:56
@article{801f0a38-bc1c-4a12-98f6-c04c1f2ef9c5,
abstract = {{The structural change of individual giant double-stranded T4 DNA (165.6 kilobase pairs) molecules as a function of salt concentration was investigated by single molecular observation with fluorescence microscopy in a wide range of concentration of NaCl, 1 x 10(-6)-3 M. The measured long-axis length was transformed into a persistence length and subjected to a power-law analysis, which showed that the electrostatic contribution to the persistence length is inversely proportional to the square root of the salt concentration; i.e., it has a linear dependence on the screening length. This is interpreted as the behavior of a flexible chain with electrostatic excluded-volume interactions. Although double-stranded DNA is locally stiff, it displays flexibility when the contour length is much longer than the persistence length, as is the case for T4 DNA.}},
author = {{Makita, Naoko and Ullner, Magnus and Yoshikawa, Kenichi}},
issn = {{0024-9297}},
language = {{eng}},
number = {{18}},
pages = {{6200--6206}},
publisher = {{The American Chemical Society (ACS)}},
series = {{Macromolecules}},
title = {{Conformational change of giant DNA with added salt as revealed by single molecular observation}},
url = {{http://dx.doi.org/10.1021/ma060669b}},
doi = {{10.1021/ma060669b}},
volume = {{39}},
year = {{2006}},
}