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Influence of temperature on process efficiency and microbial community response during the biological removal of chlorophenols in a packed-bed bioreactor

Zilouei, Hamid LU ; Soares, Ana LU ; Murto, Marika LU ; Guieysse, Benoit LU and Mattiasson, Bo LU (2006) In Applied Microbiology and Biotechnology 72(3). p.591-599
Abstract
Two reactors, initially operated at 14 and 23 +/- 1 degrees C (RA and RB, respectively), were inoculated with a bacterial consortium enriched and acclimatized to the respective temperatures over 4 months. The biofilms, formed in the reactors, were studied using scanning electron microscopy, cultivation of the biofilm microflora, and physiological analysis of the isolates. Two bacteria able to mineralize chlorophenols under a large range of temperature (10-30 degrees C) were isolated from the biofilm communities of reactors RA and RB and characterized as Alcaligenaceae bacterium R14C4 and Cupriavidus basilensis R25C6, respectively. When temperature was decreased by 10 degrees C, the chlorophenols removal capacity was reduced from 51.6 to... (More)
Two reactors, initially operated at 14 and 23 +/- 1 degrees C (RA and RB, respectively), were inoculated with a bacterial consortium enriched and acclimatized to the respective temperatures over 4 months. The biofilms, formed in the reactors, were studied using scanning electron microscopy, cultivation of the biofilm microflora, and physiological analysis of the isolates. Two bacteria able to mineralize chlorophenols under a large range of temperature (10-30 degrees C) were isolated from the biofilm communities of reactors RA and RB and characterized as Alcaligenaceae bacterium R14C4 and Cupriavidus basilensis R25C6, respectively. When temperature was decreased by 10 degrees C, the chlorophenols removal capacity was reduced from 51.6 to 22.8 mg l(-1) h(-1) in bioreactor RA (from 14 to 4 degrees C) and from 59.3 to 34.7 mg l(-1) h(-1) in bioreactor RB (from 23 +/- 1 to 14 degrees C). Fluorescence in situ hybridization (FISH) of the biofilm communities showed that, in all temperatures tested, the beta-proteobacteria were the major bacterial community (35-47%) followed by the gamma-proteobacteria (12.0-6.5%). When the temperature was decreased by 10 degrees C, the proportions of gamma-proteobacteria and Pseudomonas species increased significantly in both microbial communities. (Less)
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Applied Microbiology and Biotechnology
volume
72
issue
3
pages
591 - 599
publisher
Springer
external identifiers
  • wos:000239956200024
  • scopus:33747664935
  • pmid:16402167
ISSN
1432-0614
DOI
10.1007/s00253-005-0296-z
language
English
LU publication?
yes
id
b1db89c0-4353-4912-8815-8c8a361621cf (old id 395338)
date added to LUP
2016-04-01 16:29:25
date last changed
2021-08-11 04:33:33
@article{b1db89c0-4353-4912-8815-8c8a361621cf,
  abstract     = {Two reactors, initially operated at 14 and 23 +/- 1 degrees C (RA and RB, respectively), were inoculated with a bacterial consortium enriched and acclimatized to the respective temperatures over 4 months. The biofilms, formed in the reactors, were studied using scanning electron microscopy, cultivation of the biofilm microflora, and physiological analysis of the isolates. Two bacteria able to mineralize chlorophenols under a large range of temperature (10-30 degrees C) were isolated from the biofilm communities of reactors RA and RB and characterized as Alcaligenaceae bacterium R14C4 and Cupriavidus basilensis R25C6, respectively. When temperature was decreased by 10 degrees C, the chlorophenols removal capacity was reduced from 51.6 to 22.8 mg l(-1) h(-1) in bioreactor RA (from 14 to 4 degrees C) and from 59.3 to 34.7 mg l(-1) h(-1) in bioreactor RB (from 23 +/- 1 to 14 degrees C). Fluorescence in situ hybridization (FISH) of the biofilm communities showed that, in all temperatures tested, the beta-proteobacteria were the major bacterial community (35-47%) followed by the gamma-proteobacteria (12.0-6.5%). When the temperature was decreased by 10 degrees C, the proportions of gamma-proteobacteria and Pseudomonas species increased significantly in both microbial communities.},
  author       = {Zilouei, Hamid and Soares, Ana and Murto, Marika and Guieysse, Benoit and Mattiasson, Bo},
  issn         = {1432-0614},
  language     = {eng},
  number       = {3},
  pages        = {591--599},
  publisher    = {Springer},
  series       = {Applied Microbiology and Biotechnology},
  title        = {Influence of temperature on process efficiency and microbial community response during the biological removal of chlorophenols in a packed-bed bioreactor},
  url          = {http://dx.doi.org/10.1007/s00253-005-0296-z},
  doi          = {10.1007/s00253-005-0296-z},
  volume       = {72},
  year         = {2006},
}