Light-regulated release of liposomes from phospholipid membranes via photoresponsive polymer-DNA conjugates
(2006) In Soft Matter 2(8). p.710-715- Abstract
- A method for releasing tethered liposomes from a supported lipid bilayer in response to a light stimulus is described. The tethering is accomplished through the hybridization of end-functionalized DNA that resides on both the supported lipid bilayer and liposome surfaces. Normally consisting of cholesterol or lipid tails, the end group is replaced in this study by a photoresponsive polymer that partitions into lipid bilayers at physiological pH. When exposed to UV light, it undergoes excited state proton transfer with water. The ensuing increase in polarity increases the solubility of the polymer in the aqueous phase. Quartz crystal microbalance with dissipation monitoring (QCM-D) and fluorescence microscopy have been used to record both... (More)
- A method for releasing tethered liposomes from a supported lipid bilayer in response to a light stimulus is described. The tethering is accomplished through the hybridization of end-functionalized DNA that resides on both the supported lipid bilayer and liposome surfaces. Normally consisting of cholesterol or lipid tails, the end group is replaced in this study by a photoresponsive polymer that partitions into lipid bilayers at physiological pH. When exposed to UV light, it undergoes excited state proton transfer with water. The ensuing increase in polarity increases the solubility of the polymer in the aqueous phase. Quartz crystal microbalance with dissipation monitoring (QCM-D) and fluorescence microscopy have been used to record both the construction of the vesicle assembly and the subsequent response to UV light. It is found that the critical flow rate for vesicle release is reduced when buffer flow is performed in conjunction with UV exposure. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/395428
- author
- Benkoski, Jason J. ; Jesorka, Aldo ; Edvardsson, Malin and Höök, Fredrik LU
- organization
- publishing date
- 2006
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Soft Matter
- volume
- 2
- issue
- 8
- pages
- 710 - 715
- publisher
- Royal Society of Chemistry
- external identifiers
-
- wos:000239762500012
- scopus:33746254934
- ISSN
- 1744-6848
- DOI
- 10.1039/b606123k
- language
- English
- LU publication?
- yes
- id
- 2526c8aa-febf-47f9-815d-d6743962339d (old id 395428)
- date added to LUP
- 2016-04-01 16:31:31
- date last changed
- 2022-01-28 20:17:48
@article{2526c8aa-febf-47f9-815d-d6743962339d,
abstract = {{A method for releasing tethered liposomes from a supported lipid bilayer in response to a light stimulus is described. The tethering is accomplished through the hybridization of end-functionalized DNA that resides on both the supported lipid bilayer and liposome surfaces. Normally consisting of cholesterol or lipid tails, the end group is replaced in this study by a photoresponsive polymer that partitions into lipid bilayers at physiological pH. When exposed to UV light, it undergoes excited state proton transfer with water. The ensuing increase in polarity increases the solubility of the polymer in the aqueous phase. Quartz crystal microbalance with dissipation monitoring (QCM-D) and fluorescence microscopy have been used to record both the construction of the vesicle assembly and the subsequent response to UV light. It is found that the critical flow rate for vesicle release is reduced when buffer flow is performed in conjunction with UV exposure.}},
author = {{Benkoski, Jason J. and Jesorka, Aldo and Edvardsson, Malin and Höök, Fredrik}},
issn = {{1744-6848}},
language = {{eng}},
number = {{8}},
pages = {{710--715}},
publisher = {{Royal Society of Chemistry}},
series = {{Soft Matter}},
title = {{Light-regulated release of liposomes from phospholipid membranes via photoresponsive polymer-DNA conjugates}},
url = {{http://dx.doi.org/10.1039/b606123k}},
doi = {{10.1039/b606123k}},
volume = {{2}},
year = {{2006}},
}