Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Light-regulated release of liposomes from phospholipid membranes via photoresponsive polymer-DNA conjugates

Benkoski, Jason J. ; Jesorka, Aldo ; Edvardsson, Malin and Höök, Fredrik (2006) In Soft Matter 2(8). p.710-715
Abstract
A method for releasing tethered liposomes from a supported lipid bilayer in response to a light stimulus is described. The tethering is accomplished through the hybridization of end-functionalized DNA that resides on both the supported lipid bilayer and liposome surfaces. Normally consisting of cholesterol or lipid tails, the end group is replaced in this study by a photoresponsive polymer that partitions into lipid bilayers at physiological pH. When exposed to UV light, it undergoes excited state proton transfer with water. The ensuing increase in polarity increases the solubility of the polymer in the aqueous phase. Quartz crystal microbalance with dissipation monitoring (QCM-D) and fluorescence microscopy have been used to record both... (More)
A method for releasing tethered liposomes from a supported lipid bilayer in response to a light stimulus is described. The tethering is accomplished through the hybridization of end-functionalized DNA that resides on both the supported lipid bilayer and liposome surfaces. Normally consisting of cholesterol or lipid tails, the end group is replaced in this study by a photoresponsive polymer that partitions into lipid bilayers at physiological pH. When exposed to UV light, it undergoes excited state proton transfer with water. The ensuing increase in polarity increases the solubility of the polymer in the aqueous phase. Quartz crystal microbalance with dissipation monitoring (QCM-D) and fluorescence microscopy have been used to record both the construction of the vesicle assembly and the subsequent response to UV light. It is found that the critical flow rate for vesicle release is reduced when buffer flow is performed in conjunction with UV exposure. (Less)
Please use this url to cite or link to this publication:
author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Soft Matter
volume
2
issue
8
pages
710 - 715
publisher
Royal Society of Chemistry
external identifiers
  • wos:000239762500012
  • scopus:33746254934
ISSN
1744-6848
DOI
10.1039/b606123k
language
English
LU publication?
yes
id
2526c8aa-febf-47f9-815d-d6743962339d (old id 395428)
date added to LUP
2016-04-01 16:31:31
date last changed
2021-10-06 03:56:21
@article{2526c8aa-febf-47f9-815d-d6743962339d,
  abstract     = {A method for releasing tethered liposomes from a supported lipid bilayer in response to a light stimulus is described. The tethering is accomplished through the hybridization of end-functionalized DNA that resides on both the supported lipid bilayer and liposome surfaces. Normally consisting of cholesterol or lipid tails, the end group is replaced in this study by a photoresponsive polymer that partitions into lipid bilayers at physiological pH. When exposed to UV light, it undergoes excited state proton transfer with water. The ensuing increase in polarity increases the solubility of the polymer in the aqueous phase. Quartz crystal microbalance with dissipation monitoring (QCM-D) and fluorescence microscopy have been used to record both the construction of the vesicle assembly and the subsequent response to UV light. It is found that the critical flow rate for vesicle release is reduced when buffer flow is performed in conjunction with UV exposure.},
  author       = {Benkoski, Jason J. and Jesorka, Aldo and Edvardsson, Malin and Höök, Fredrik},
  issn         = {1744-6848},
  language     = {eng},
  number       = {8},
  pages        = {710--715},
  publisher    = {Royal Society of Chemistry},
  series       = {Soft Matter},
  title        = {Light-regulated release of liposomes from phospholipid membranes via photoresponsive polymer-DNA conjugates},
  url          = {http://dx.doi.org/10.1039/b606123k},
  doi          = {10.1039/b606123k},
  volume       = {2},
  year         = {2006},
}